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Carbon nano tube/nanogold composite membrane electrochemical immunosensor and application thereof

An immunosensor and carbon nanotube technology, applied in the field of electrochemical analysis, can solve the problems of complicated operation, narrow linear range, low sensitivity, etc., and achieve the effects of good selectivity, high accuracy and high sensitivity

Inactive Publication Date: 2015-04-08
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the commonly used detection methods for microcystins in water mainly include high-performance liquid chromatography and high-performance liquid chromatography-mass spectrometry, and a series of valuable results have been obtained, but the equipment is expensive and the operation is cumbersome. And the technical requirements for operators are high; the sensitivity of plant cell biological assay and protein phosphatase inhibition method is relatively low; the sensitivity of enzyme-linked immunosorbent assay is high, but its linear range is narrow

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  • Carbon nano tube/nanogold composite membrane electrochemical immunosensor and application thereof
  • Carbon nano tube/nanogold composite membrane electrochemical immunosensor and application thereof
  • Carbon nano tube/nanogold composite membrane electrochemical immunosensor and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 Preparation of carbon nanotube / gold nanocomposite film and its immunosensor

[0026] The first step: glassy carbon electrode (diameter 3 mm) with Al 2 o 3 Polish to a mirror surface, and then ultrasonically clean with acetone, nitric acid solution (1.0 mol / L), sodium hydroxide solution (1.0 mol / L) and double distilled water for 5 min. After the treated glassy carbon electrode was blown dry, 10 μL of carbon nanotube solution was drip-coated and dried under an infrared lamp. The prepared glassy carbon electrode / carbon nanotube was placed in a sulfuric acid solution containing 0.10 mmol / L chloroauric acid ( 0.50 mol / L), deposited nano-gold on the electrode surface by multi-potential step method (scanned for 15 s in the step potential range of 1.055 ~ –0.045 V) to prepare glassy carbon electrode / carbon nanotube / nano-gold. use;

[0027] Step 2: After the modified electrode was washed with double distilled water and dried, 10 μL of microcystin antibody was applie...

Embodiment 2

[0030] Example 2 Constructing an electrochemical immunosensor based on a carbon nanotube / gold nanocomposite film for the detection of microcystins in water

[0031] After the immunosensor was cleaned and dried, it was put into a certain concentration of microcystin solution and incubated at 37 oC for 60 min. After washing the electrode with PBS, 10 μL of horseradish peroxidase-labeled microcystin antibody was dropped on the surface of the electrode and incubated for 60 min. Then, the electrode was washed with water and placed in 5 mL of PBS containing 0.80 mmol / L hydrogen peroxide and 0.50 mmol / L hydroquinone, and quantitatively detected by differential pulse voltammetry with a scan range of 0.40 to –0.6 V. Using hydroquinone as the electron mediator, the detection of microcystin was realized through the response current generated by hydrogen peroxide catalyzed by horseradish peroxidase.

[0032] image 3 is the response current map of the electrochemical immunosensor based ...

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Abstract

The invention discloses a carbon nano tube / nanogold composite membrane electrochemical immunosensor and application thereof, belonging to the technical field of electrochemical analysis. The surface of a glass carbon electrode is modified by a carbon nano tube, and a multi-potential-step method is adopted for depositing nano gold on the surface of the carbon nano tube, and thus the carbon nano tube / nanogold composite membrane is obtained; under the adsorption effect between nanogold and microcystic toxin-(leucine-arginine) antibody, an antibody is fixed on the surface of an electrode, a non-specific adsorption site is closed by utilizing bovine serum albumin, and an electrochemical immunosensor used for detecting microcystic toxin is researched; an immunoassay analysis mode of a 'sandwich'-structure is constructed by utilizing specific recognition effect between the microcystic toxin and an antibody thereof; and horse radish peroxidase labelled antibody is taken as a detection beacon, and detection on microcystic toxin is realized by utilizing a differential pulse voltammetry method. The carbon nano tube / nanogold composite membrane electrochemical immunosensor has high detection sensitivity, good selectivity and high accuracy when being used for microcystic toxin detection, and a new method is provided for analysis and study of microcystic toxin in water body.

Description

technical field [0001] The present invention relates to a method for electrochemical immunoassay, to be precise, relates to a research on the use of carbon nanotube / nano-gold composite material to establish an electrochemical immunosensor, which is applied to the detection of microcystins in water bodies, and belongs to electrochemical analysis technology field. Background technique [0002] Microcystins are a class of highly toxic and acutely harmful cyclic heptapeptide hepatotoxins produced by freshwater blue-green algae, which have serious impacts on aquatic organisms, drinking water safety and human health. Among them, microcystin-(leucine-arginine) (MCLR) is one of the most common and acutely toxic microcystins. The content of microcystins in water bodies has been used by many countries and organizations as an important indicator to measure water quality standards. [0003] At present, the commonly used detection methods for microcystins in water mainly include high-p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/551G01N27/327
CPCG01N33/551G01N27/327
Inventor 张新爱鲁文杰耿萍申建忠蒋玉香
Owner JIANGSU UNIV
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