Habituation culture method of psychrophilic methanogens

A technology for the domestication and cultivation of methanogens, which is applied in the field of domestication and cultivation of psychrophilic methanogens, can solve the problems of incapability of large-scale production and high investment costs, and achieve the effects of improving stability, improving fermentation efficiency, and efficient metabolic activity.

Active Publication Date: 2015-04-08
GANSU DEFU BIOTECH
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  • Application Information

AI Technical Summary

Problems solved by technology

That is to say, it takes about 20 days for the psychrophilic methanogenic bacteria method disclosed in the report to enrich the generational flora, and the methane production is only 30%. For enterprises, the investment cost is relatively high and cannot be scaled up. chemical production

Method used

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Embodiment 1

[0029] A method for acclimating and cultivating psychrophilic methanogens, which controls temperature sawtooth rise and fall and feeds at a constant temperature, and overcomes the cold shock response of psychrophilic methanogens in temperature changes. The specific steps are:

[0030] 1. Prepare acclimatization medium, its raw material components and ratio are: MgCl 2 0.1~0.3g / L, NaCl 3.0~5.0g / L, NH 4 Cl 1.0~2.0g / L, KH 2 PO 4 4.0~5.0g / L,K 2 HPO 4 4.0~5.0g / L, Giemsa stock solution 4.5~6.0ml / L, cysteine ​​0.5~0.8g / L, yeast extract 5.0~8.0g / L, inorganic salt solution 25~28ml / L, vitamin solution 8~10ml / L, trace element solution 7~9ml / L, pH in the range of 6.8~7.2.

[0031] The component of described inorganic salt solution is: KH 2 PO 4 7.5~8.0g / L, (NH 4 ) 2 SO 4 7.5~8.0g / L,K 2 HPO 4 6.5~7.0g / L, NaCl 8.0~10.0g / L, MgSO 4 7.0~9.0g / L, CaCl 2 7.0~9.0g / L.

[0032] The vitamin liquid components are: glutamic acid 0.015g / L, ascorbic acid 0.02g / L, riboflavin 0.025g / L, citr...

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Abstract

The invention discloses a habituation culture method of psychrophilic methanogens. The habituation culture method comprises the following steps: 1) feeding a habituation culture medium into a culture device, introducing nitrogen to remove oxygen, sterilizing at high temperature, and adding 0.001g of sodium acetate, sodium formate, methanol, cellulose acetate, chitosan and prebiotics respectively, thereby obtaining a habituation culture device; 2) crushing sludge bacteria, adding activated starch, and putting the mixture to stand, thereby obtaining an activated starch-sludge bacterium; 3) inoculating the bacterium obtained in the step 2) to the habituation culture device of the step 1), and culturing for 4-5 days at 8 DEG C; 4) detecting, changing the temperature through a temperature control system when bubbles are generated, and implementing temperature-variable habituation culture; and 5) stopping temperature variation when the bubbles are not increased any more, and replenishing materials to generate bubbles continuously until the gas generation amount is completely stable after 12 days, thereby completing enrichment of psychrophilic methanogens. By adopting the habituation culture method of psychrophilic methanogens, the formation speed of bacterial colonies is remarkably increased, and the enrichment effect is improved.

Description

technical field [0001] The invention belongs to the technical field of microorganism culture, and in particular relates to a domestication culture method of psychrophilic methanogens. Background technique [0002] With the reduction of available natural resources and the aggravation of environmental pollution, biomass energy has become one of the most promising energy sources to solve the resource problems faced by human beings. Biogas fermentation uses forestry, agricultural waste and urban waste as raw materials to produce green and renewable resources, which is of great significance to the harmonious development of society and the environment. The biogas fermentation process is a complex biochemical process that is combined and alternated by a variety of microorganisms. The temperature, moisture, raw materials, pH and airtightness of the fermentation process are the necessary conditions to ensure the continuous progress of the biochemical process, and the temperature is t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36
CPCC12N1/36
Inventor 常贵卢新社
Owner GANSU DEFU BIOTECH
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