A method for purification and rejuvenation of edible fungus strains

Abstract

The invention relates to a method for the purification and rejuvenation of an edible fungus strain. According to the method, a first culture medium and a second culture medium are adopted, wherein the first culture medium is obtained by mixing 95 to 97 weight percent of sawdust and 3 to 5 weight percent of river silt and regulating the moisture content of the mixture with a 1 percent potassium permanganate aqueous solution, and the second culture medium is obtained by mixing 95 to 97 weight percent of sawdust and 3 to 5 weight percent of river silt and regulating the moisture content with a 0.5 percent topsin aqueous solution. The method comprises the following steps: pushing 80 grams of the first culture medium and 80 grams of the second culture medium into the centers of bottomless test tubes respectively, and plugging cotton plugs into the two ends of the bottomless test tubes for sterilization; selecting fungus blocks sized as green beans in an aseptic technique manner, inoculating the fungus blocks into the first culture medium, performing culturing for 4 to 6 days at 25 DEG C, selecting mycelium at the uninoculated end to be inoculated to one end of the second culture medium when the mycelium in the first culture medium reaches the other end, performing culturing for 4 to 6 days at 25 DEG C, selecting the mycelium at the uninoculated end to be inoculated to a PDA culture medium when the mycelium penetrates through the second culture medium, and performing culturing for 4 days at 25 DEG C to obtain sterile mother strain. The method is short in cycle and high in accuracy, the sources are simple, the virus, bacterium and fungus pollution of the strain can be rapidly removed, and meanwhile, rejuvenation and strain purification functions are realized.

Description

technical field [0001] The invention relates to the technical field of edible fungi, in particular to a method for purifying and rejuvenating edible fungus strains. technical background [0002] With the rapid development of industrial cultivation of edible fungi in recent years, the control of strain quality is becoming more and more important. In the continuous production process, the original mother species of edible fungi are prone to virus, bacteria, fungal contamination and species degradation. After the edible fungus strains are polluted or degraded, it will lead to a large reduction in output or even extinction in the later stage. The degradation of edible fungus strains is extremely difficult to judge from the senses. In many edible fungi factories, there have been incidents in which edible fungus strains have been polluted and degraded, leading to production reduction or even extinction. At present, for the pollution and degradation of edible fungus strains, the m...

AI Technical Summary

Problems solved by technology

In the continuous production process, the original mother species of edible fungi are prone to virus, bacteria, fungal contamination and species degradation. After the edible fungi are polluted or degraded, it will lead to a large reduction in output or even extinction in the later stage.

The degradation of edible fungus strains is extremely difficult to judge from the senses. In many edible fungi factories, there have been incidents in which edible fungus strains have been polluted and degraded, leading to production reduction or even extinction.

At present, for the pollution and degradation of edible fungus strains, the methods of tip detoxification and bridge-breaking detoxification are mostly used. In actual production, it is found that the ability of tip detoxification and bridge-breaking detoxification is limited, and the safety and reliability of the strains cannot be fully guaranteed. At the same time Tip detoxification and broken bridge detoxification can not play the role of rejuvenation of edible fungi