A kind of washing composite enzyme containing fungal α-amylase and preparation method thereof
An amylase and compound enzyme technology, applied in the field of washing compound enzymes, can solve the problems of single function, single type of washing enzyme, poor storage stability, etc.
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Embodiment 1
[0055] Embodiment 1 raw material preparation
[0056] 1. Preparation of fungal α-amylase:
[0057] The preparation method of the fungal alpha-amylase comprises the following steps:
[0058] (1) Activation of bacteria
[0059] Inoculate the well-preserved slant strain of Trichoderma reesei CCTCC NO:M 2013602 on the slant medium, culture at 30°C for 40 hours to activate the strain, and activate it 3 times;
[0060] (2) Expanded cultivation of liquid seeds
[0061] ①First-level seed culture: wash the spores of the slanted bacteria after activation in step (1) with sterile water, insert them into a 500-ml shaker flask, fill the liquid seed medium with 100 ml, and culture on a shaker at 30°C and 100rpm for 40 hours;
[0062] ②Cultivation of secondary seeds: Inoculate 10% of the primary seeds into 500 ml secondary seed shake flasks, and the cultivation conditions are the same as those of the primary seeds;
[0063] ③Third-level seed cultivation: insert the second-level seeds int...
Embodiment 2
[0081] A washing complex enzyme containing fungal α-amylase, prepared from the raw materials of the following weight components:
[0082] 50 parts of sawdust, 30 parts of fungal α-amylase, 25 parts of alkaline protease, 25 parts of alkaline lipase, 20 parts of plant extract, 6 parts of alkaline cellulase, 5 parts of nonionic surfactant, 4 parts of activator part, 2 parts of protective agent, 0.4 part of antioxidant;
[0083] The sawdust, fungal α-amylase, and plant extract are all prepared in Example 1;
[0084] The mass components of the nonionic surfactant are: 35 parts of alkyl polyglycosides (APG), 25 parts of alkyl glucamides (AGA), 10 parts of N-dodecyl ethylenediamine triacetate (ED3A) , isomerized alcohol ether carboxylate AEC-110710 parts, lauramide ether carboxylate (LAEC) 7 parts, Pingpingjia c-1251 parts, JFC 1 part;
[0085] The activator is formed by uniformly mixing inorganic salts with the following mass components: 20 parts of sodium sulfate, 12 parts of cal...
Embodiment 3
[0090] A washing complex enzyme containing fungal α-amylase, prepared from the raw materials of the following weight components:
[0091] 45 parts of sawdust, 25 parts of fungal alpha-amylase, 20 parts of alkaline protease, 20 parts of alkaline lipase, 15 parts of plant extract, 3 parts of alkaline cellulase, 2 parts of nonionic surfactant, 2 parts of activator 1 part, 1 part of protective agent, 0.3 part of antioxidant;
[0092] The sawdust, fungal α-amylase, and plant extract are all prepared in Example 1;
[0093] The mass components of the nonionic surfactant are: 30 parts of alkyl polyglycosides (APG), 20 parts of alkyl glucamides (AGA), 8 parts of N-dodecyl ethylenediamine triacetate (ED3A) , isomerized alcohol ether carboxylate AEC-11078 parts, lauramide ether carboxylate (LAEC) 5 parts;
[0094] The activator is formed by uniformly mixing inorganic salts with the following mass components: 15 parts of sodium sulfate, 10 parts of calcium chloride, 5 parts of zinc chlo...
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