A DNA fluorescence spectrum acquisition method based on quantitative analysis of characteristic molecular weight internal standard
A technology of molecular weight internal standard and fluorescence spectrum, which is applied in the field of DNA fluorescence spectrum acquisition based on the quantitative analysis of characteristic molecular weight internal standard, can solve the problems of waste of memory and acquisition time, data access, data processing and data analysis time extension, etc., to reduce Data processing capacity, save data collection time, and improve work efficiency
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[0043] The present invention is aimed at forensic DNA analysis instruments, and provides a DNA fluorescence spectrum collection method based on the quantitative analysis of the characteristic molecular weight internal standard in combination with the actual needs of the DNA detection business and the characteristics of the fluorescence spectrum signal. Save data collection time, and then save the memory occupied by the source data, reduce the amount of subsequent data processing, and ultimately improve the efficiency of DNA identification.
[0044] Typically, the signal acquired by the DNA analysis instrument adequately covers the required detection range. An original DNA fluorescence spectrum signal is divided into three sections, which include the first primer peak section, the subsequent useful DNA signal peak section, and the redundant data section beyond the detection range of the internal standard, such as figure 1 shown.
[0045] The DNA fluorescence spectrum acquisiti...
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