Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A DNA fluorescence spectrum acquisition method based on quantitative analysis of characteristic molecular weight internal standard

A technology of molecular weight internal standard and fluorescence spectrum, which is applied in the field of DNA fluorescence spectrum acquisition based on the quantitative analysis of characteristic molecular weight internal standard, can solve the problems of waste of memory and acquisition time, data access, data processing and data analysis time extension, etc., to reduce Data processing capacity, save data collection time, and improve work efficiency

Active Publication Date: 2017-01-18
THE FIRST RES INST OF MIN OF PUBLIC SECURITY +1
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the number of data acquisition frames of DNA analysis instruments is a constant, which stipulates a fixed number of data acquisition frames for the detection business. Although this can fully cover the required detection range, the useless data corresponding to the actual detection range is undoubtedly wasted. memory and acquisition time, and thus lead to an innocent increase in subsequent data access, data processing, and data analysis time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A DNA fluorescence spectrum acquisition method based on quantitative analysis of characteristic molecular weight internal standard
  • A DNA fluorescence spectrum acquisition method based on quantitative analysis of characteristic molecular weight internal standard
  • A DNA fluorescence spectrum acquisition method based on quantitative analysis of characteristic molecular weight internal standard

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0043] The present invention is aimed at forensic DNA analysis instruments, and provides a DNA fluorescence spectrum collection method based on the quantitative analysis of the characteristic molecular weight internal standard in combination with the actual needs of the DNA detection business and the characteristics of the fluorescence spectrum signal. Save data collection time, and then save the memory occupied by the source data, reduce the amount of subsequent data processing, and ultimately improve the efficiency of DNA identification.

[0044] Typically, the signal acquired by the DNA analysis instrument adequately covers the required detection range. An original DNA fluorescence spectrum signal is divided into three sections, which include the first primer peak section, the subsequent useful DNA signal peak section, and the redundant data section beyond the detection range of the internal standard, such as figure 1 shown.

[0045] The DNA fluorescence spectrum acquisiti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a DNA fluorescence spectrum collecting method based on characteristic molecular weight interior label quantitative analysis. The method is used for performing data acquisition on a detecting sample which simultaneously contains an interior label sample-adding marker DNA fragment and unknown DNA fragment length information, and also used for performing real-time processing on the acquired initial data. The DNA fluorescence spectrum collecting method based on characteristic molecular weight interior label quantitative analysis comprises the following steps: firstly, performing primer peak identification; then, performing spectral correction point by point on the acquired signal after the primer peak, and performing peak identification on a corresponding interior label channel signal to match with a characteristic molecular weight peak until the characteristic peak with the greatest molecular weight of the interior label is extracted; and finally, according to the peak position corresponding to the greatest molecular weight, determining an ending moment of the DNA fluorescence spectrum data acquisition. The method can be used for reducing the DNA fluorescence spectrum data acquisition time, saving the internal storage occupied by source data, reducing the subsequent processed data and finally improving the DNA identification working efficiency.

Description

technical field [0001] The invention relates to the technical field of DNA detection, in particular to a DNA fluorescence spectrum collection method based on the quantitative analysis of a characteristic molecular weight internal standard. Background technique [0002] With the rapid development of science and technology, the rapid rise of the era of big data poses many challenges to the manufacture of modern analytical instruments and instrument analysis methods. At present, the demand for DNA analysis business and the rapid growth of biological information data, how to improve the speed of data access, save the memory occupied by data, and improve the maintenance of the database are the keys to the development of DNA biological research. If the big data DNA biological information access and processing technology stagnates, it will seriously limit the utilization of data, thereby hindering the development of the entire DNA instrument analysis technology. [0003] Undoubted...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 贾二惠张涛李彬赵怡鹤
Owner THE FIRST RES INST OF MIN OF PUBLIC SECURITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products