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Horseradish-peroxidase-mediated free radical initiation system and method for preparing hydrogel

A technology of horseradish peroxidase and initiation system, applied in the polymer field, can solve problems such as unfavorable application, high biological toxicity of acetylacetone, no reports, etc., and achieve the effects of excellent mechanical properties and biocompatibility

Inactive Publication Date: 2015-03-25
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high biological toxicity of acetylacetone is not conducive to its application in biomaterials
At present, no acetylacetone-free horseradish peroxidase-mediated free radical polymerization initiation system has not been reported

Method used

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  • Horseradish-peroxidase-mediated free radical initiation system and method for preparing hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Preparation of precursor solution: Take 0.11-0.13g of N,N-dimethylacrylamide, 0.07-0.09g of cross-linking agent polyethylene glycol diacrylate (average molecular weight 250), and 1.6-1.7g of deionized water into the sample bottle, mix well with a vortex mixer.

[0025] 2) Preparation of hydrogel: 0.66 mg of N-hydroxysuccinimide, 100 μL of horseradish peroxidase concentrate, and 50 μL of aqueous hydrogen peroxide (N-hydroxysuccinimide in the reaction system) were sequentially added to the above precursor solution. The molar concentration ratio of amine and hydrogen peroxide is 4.35, the molar concentration ratio of hydrogen peroxide and horseradish peroxidase is 25, and the final molar concentration of hydrogen peroxide is 0.66mM), fast mixing is timed with a stopwatch simultaneously, airtight static Place to obtain a light brown-red transparent hydrogel, and the gelation time is 3min10s.

Embodiment 2

[0027] 1) Preparing the precursor solution: the steps are the same as in Example 1.

[0028] 2) Preparation of hydrogel: Add 0.61 mg of N-hydroxysuccinimide, 100 μL of horseradish peroxidase concentrate, and 50 μL of aqueous hydrogen peroxide (N-hydroxysuccinimide in the reaction system The molar concentration ratio of amine and hydrogen peroxide is 2, the molar concentration ratio of hydrogen peroxide and horseradish peroxidase is 25, and the final molar concentration of hydrogen peroxide is 1.32mM), mixes quickly, and airtightly stands still to obtain light brown Red transparent hydrogel, gelation time 2min20s.

Embodiment 3

[0030] 1) Preparing the precursor solution: the steps are the same as in Example 1.

[0031] 2) Preparation of hydrogel: Add 1.77 mg of N-hydroxysuccinimide, 100 μL of horseradish peroxidase concentrate, and 50 μL of aqueous hydrogen peroxide (N-hydroxysuccinimide in the reaction system The molar concentration ratio of amine and hydrogen peroxide is 5.84, and the molar concentration ratio of hydrogen peroxide and horseradish peroxidase is 25, and the final molar concentration of hydrogen peroxide is 1.32mM), mix quickly, airtight and stand still to obtain light brown Red transparent hydrogel, gelation time 1min40s.

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Abstract

The invention relates to a horseradish-peroxidase-mediated free radical initiation system and a method for preparing a hydrogel. The initiation system is composed of N-hydroxysuccinimide, horseradish peroxidase and hydrogen peroxide. By employing N-hydroxysuccinimide as an enzyme substrate, acetylacetone with relatively large biological toxicity is avoided. The preparation method is capable of initiating a monomer within 50 s-5 min at room temperature for preparing the hydrogel by adjusting the concentration ratio of the three-composition initiation system, is applicable to prepare a high-strength nanometer composite hydrogel, has the advantages of being friendly to environment and rapid and controllable in gel forming, and has obvious application prospect on the fields of medicine controlled release, enzyme immobilization, tissue engineering and the like.

Description

technical field [0001] The invention relates to the technical field of macromolecules, in particular to a horseradish peroxidase-mediated free radical initiation system and a method for preparing hydrogel using the system. Background technique [0002] Free radical polymerization is often used in the production of high molecular polymers and the manufacture of fine chemicals, and is an important technology for industrial production of high molecular products. The first step in a free radical reaction is free radical initiation. The traditional free radical initiation methods mainly include: thermal initiation; redox initiation; ultraviolet light initiation; high-energy radiation (such as laser, α-ray, β-ray, γ-ray, X-ray irradiation, etc.) initiation and so on. These polymerization initiation methods either require the use of special equipment and consume a large amount of energy, or require the use of some toxic initiators, which are harmful to organisms and may affect the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02
Inventor 苏腾王启刚
Owner TONGJI UNIV
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