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Biochar immobilized enzyme for converting waste kitchen oil into biodiesel and preparation method thereof

A technology of biodiesel and kitchen waste oil, which is applied in the direction of fixing on or in the inorganic carrier, fixing on/in the organic carrier, etc., can solve rare problems, and achieve simple and operable application process Strong, low-cost application effect

Inactive Publication Date: 2015-03-25
NINGBO URBAN ENVIRONMENT OBSERVATION & RES STATION NUEORS CHINESE ACADEMY OF SCI +1
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  • Abstract
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Problems solved by technology

At present, there are not many studies on the use of biochar carriers for immobilized lipase, especially in the conversion of waste oil from urban kitchens to biodiesel.

Method used

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Examples

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Embodiment Construction

[0012] The implementation of the present invention will be described in detail below in conjunction with specific examples, so as to fully understand and implement the process of how to apply technical means to solve technical problems and achieve technical effects in the present invention.

[0013] Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the reagents and products used are also commercially available. The sources, trade names and, where necessary, listing of constituents of reagents used are indicated the first time they appear.

[0014] Embodiments of the invention are:

[0015] A preparation method of chitosan-modified biochar-immobilized lipase, the specific operation steps are as follows: 1) Soak biomass such as peanut shells in water, remove dust and impurities, dry them in an oven at 80°C, and pulverize them. 100-mesh sieve, put into a crucible with a cover and transfer to a ...

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Abstract

The invention provides a method for preparing a chitosan modified biochar-based immobilized enzyme which can be used for converting high acid-value waste kitchen oil into biodiesel. Peanut shells and other biomasses are subjected to high-temperature pyrolysis to form biochar which is of a porous structure under the oxygen-limited condition, the pore structure of the biochar is further modified by adopting chitosan with high biocompatibility, chitosan macromolecules are mutually cross-linked to generate a matrix material which is of a three-dimensional meshed structure, immobilization of lipase molecules on a matrix carrier is promoted, and the catalytic activity and stability of the immobilized enzyme are improved. The catalytic activity of the immobilized lipase prepared by the method is fully improved, and the prepared immobilized enzyme can be used for a catalytic system for converting the high acid-value waste kitchen oil into biodiesel.

Description

technical field [0001] The invention relates to the technical field of enzyme immobilization, in particular to a preparation method of chitosan-modified biochar-immobilized lipase which can be used for converting kitchen waste oil into biodiesel. Background technique [0002] With the continuous advancement of my country's urbanization process, the pollution load of urban kitchen waste is increasing day by day, among which waste oils and fats pose a serious threat to urban environmental sanitation and food safety due to environmental exposure and improper disposal. At present, the treatment methods of food waste mainly include direct crushing as feed or direct extraction of waste oil for reuse, incineration as domestic waste, landfill, aerobic composting, anaerobic digestion, etc. Among them, anaerobic digestion technology has received widespread attention because it can not only reduce and harmless food waste, but also realize high-value utilization. Through the anaerobic ...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10
Inventor 胡朝华万顺刚张又弛罗文邃
Owner NINGBO URBAN ENVIRONMENT OBSERVATION & RES STATION NUEORS CHINESE ACADEMY OF SCI
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