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Separation of arbuscular mycorrhizal fungi as well as preparation and application of arbuscular mycorrhizal fungi fungicide

A technology of arbuscular mycorrhizal fungi and bacterial agents, which is applied in the field of isolation of arbuscular mycorrhizal fungi, and can solve problems such as production methods that have not been commercialized

Active Publication Date: 2015-03-25
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pot culture method and spray culture method are the main methods for commercial production. The in vitro dual culture method of AMF is an ideal method to obtain pure AMF and study the genetic, physiological and biochemical characteristics of AMF, but it has not yet been obtained. commercial production method

Method used

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  • Separation of arbuscular mycorrhizal fungi as well as preparation and application of arbuscular mycorrhizal fungi fungicide
  • Separation of arbuscular mycorrhizal fungi as well as preparation and application of arbuscular mycorrhizal fungi fungicide
  • Separation of arbuscular mycorrhizal fungi as well as preparation and application of arbuscular mycorrhizal fungi fungicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Isolation and identification of arbuscular mycorrhizal fungus R. irregularis CD-1

[0050] The inventors of the present invention, in view of the above-mentioned prior art, collected soil samples of corn fields from the experimental field of Shandong Agricultural University in Tai'an City, Shandong Province, and used wet sieve pouring method after drying in the shade (Guo Xiuzhen, Bi Guochang. Forest mycorrhiza and application technology. Beijing: China Forestry Publishing House, 1989.216-220) The spores of arbuscular mycorrhizal fungi were isolated from the soil samples, and then the spores were selected with a dissecting needle under a dissecting microscope. Combining traditional morphology and PCR-based modern technology to classify and identify AMF spores. The spores were classified according to their morphological characteristics under a stereo microscope, and spores with the characteristics of arbuscular mycorrhizal fungi were selected.

[0051] Extract gen...

Embodiment 2

[0053] Example 2: Dual cultivation of arbuscular mycorrhizal fungi and Ri T-DNA carrot roots and preparation of AMF inoculum

[0054] The present invention uses the isolated spores of Rhizophagus irregularis CD-1 after surface disinfection and Agrobacterium rhizogenes to induce the cells to integrate Ri T-DNA carrot roots on the M medium ( G.,and JAFORTIN.Early events of vesiculararbuscular mycorrhiza formation on Ri T-DNA transformed roots.New Phytologist,1988,108:211218; St-Arnaud M, Hamel C, Vimard B, Caron M, Fortin JA. Enhanced hyphal growth and spore production of the arbuscular mycorrhizal fungus Glomus intraradices in an in vitro system in the absence of host roots. Mycol Res, 1996, 100: 328332; Bi Yinli, Li Xiaolin, Wang Honggang. VA mycorrhizal fungus transfers Ri T-DNA carrot root organs Infection[J].Journal of Plant Nutrition and Fertilizer,1999,5(1):76-80; Bi Yinli, Wang Honggang, Li Xiaolin. The dual culture method of arbuscular mycorrhiza and the establishment of m...

Embodiment 3

[0060] Example 3: Effects of Arbuscular Mycorrhizal Fungi on the Growth and Development of Five Tomatoes

[0061] In order to determine what effect the arbuscular mycorrhizal fungus Rhizophagus irregularis CD-1 has on the growth and development of tomatoes, we used five different tomato varieties as materials and inoculated Rhizophagus irregularis CD-1 through a pot experiment to study the symbiosis between different tomato varieties and AMF Efficiency, and the growth-promoting effect of AMF on five tomato varieties.

[0062] (1) Preparation of seedling substrate

[0063] The seedling substrate is a mixture of vermiculite, peat and perlite in a volume ratio of 3:4:4. The seedling substrate is sterilized at 121°C for 30 minutes, and when it is naturally cooled to room temperature, it is placed in a bowl with a volume of 600 cm3. Four places were inoculated with AMF inocula containing 900 spores, and then filled the bowl with the seedling substrate; at the same time, the seedling subs...

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Abstract

The invention discloses separation of arbuscular mycorrhizal fungi as well as preparation and application of arbuscular mycorrhizal fungi fungicide, and belongs to the technical field of biology. The arbuscular mycorrhizal fungi is arbuscular mycorrhizal fungi rhizophagus irregularis which is called arbuscular mycorrhizal fungi rhizophagus irregularis CD-1 and is separated from soils; a specific sequence of verifying ribosome 28S rDNA is shown in SEQ ID NO.1; by utilizing the fungicide prepared by the fungi, the height of strains, the number of petioles, the overground fresh weight, the overground dry weight, the root fresh weight and the root dry weight of tomatoes can be increased, and the mycorrhizal infection rate delta gt can be obviously prompted; 80 percent of corns are high in selfing line strains and heavy in overground fresh weight and overground dry weight; the resistance to banded sclerotial blight by the corns is improved, and the arbuscular mycorrhizal fungi has an extremely-high genetic use value.

Description

Technical field [0001] The present invention relates to the separation of arbuscular mycorrhizal fungi, preparation of inoculants and their applications, belonging to the field of biotechnology, and provides an arbuscular mycorrhizal fungus Rhizophagus irregularis CD- 1). The preparation method of the bacterial agent and its application on corn and tomato. Background technique [0002] Arbuscular Mycorrhiza Fungi (AMF) is a beneficial soil fungus belonging to the Gtomeromycota phylum. It forms a mutually beneficial symbiont Arbuscular Mycorrhiza (AMF) with about 80% of the roots of land plants. The symbiosis and interaction between plants and AMF is mainly manifested in: AMF obtains carbohydrates from plant roots to maintain its own growth and complete life history, but at the same time it can promote plants to absorb water and mineral nutrients, and can enhance plants to biological and non-biological Stress tolerance, which in turn promotes the growth and development of host pl...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N65/04A01P21/00A01G7/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 丁新华储昭辉张霞
Owner SHANDONG AGRICULTURAL UNIVERSITY
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