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Nanofiber foam-based protein adsorptive separation device and preparation method thereof

A nanofiber, adsorption and separation technology, applied in separation methods, peptide preparation methods, solid adsorbent liquid separation, etc., can solve the problems of small specific surface area of ​​adsorption matrix materials, single type of protein, and low protein dissolution rate, etc., to achieve The structure of the device can be designed, the broad application prospects, and the effect of high protein dissolution rate

Active Publication Date: 2015-03-25
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned patents all have shortcomings such as small specific surface area, low porosity, large mass transfer resistance, and single protein type of the adsorption matrix material, resulting in low adsorption capacity of the material, slow adsorption rate, low protein dissolution rate, and the drive required for separation. High energy consumption, low production efficiency and high production cost
Domestic CN203048837U discloses and reports a kind of batch protein separation and purification device, and this device assembles multiple groups of processing containers, chromatographic columns, and solution collectors after separation side by side, and relies on gravity drive and siphon drive to realize protein separation and integration operations, although The driving energy consumption is reduced, but only relying on gravity and siphon drive makes the production efficiency of the device low, and the device structure is complicated and the preparation cost is high

Method used

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  • Nanofiber foam-based protein adsorptive separation device and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Such as figure 1 As shown, it is a nanofiber foam-based protein adsorption and separation device, and the nanofiber foam-based protein adsorption and separation device includes a perforated protective cover 1, and the perforated protective cover 1 is provided with a nanofiber foam-based protein adsorption material 4. The foam-based protein adsorption material 4 is provided with a liquid-collecting capillary network 2, and the liquid-collecting capillary network 2 is connected to the main infusion pipe 3, and the main infusion pipe 3 is connected to the liquid collection tank 6 through the pump 5.

[0034] The preparation method of the above-mentioned nanofiber foam-based protein adsorption and separation device, the specific steps are:

[0035] The first step: directly embed the liquid collection capillary network 2 in the nanofiber foam during the preparation of the nanofiber foam material; the liquid collection capillary network 2 includes the main pipe and the branch...

Embodiment 2

[0044] Such as figure 1 As shown, it is a nanofiber foam-based protein adsorption and separation device, and the nanofiber foam-based protein adsorption and separation device includes a perforated protective cover 1, and the perforated protective cover 1 is provided with a nanofiber foam-based protein adsorption material 4. The foam-based protein adsorption material 4 is provided with a liquid-collecting capillary network 2, and the liquid-collecting capillary network 2 is connected to the main infusion pipe 3, and the main infusion pipe 3 is connected to the liquid collection tank 6 through the pump 5.

[0045] The preparation method of the above-mentioned nanofiber foam-based protein adsorption and separation device, the specific steps are:

[0046] The first step: directly embed the liquid collection capillary network 2 in the nanofiber foam during the preparation of the nanofiber foam material; the liquid collection capillary network 2 includes the main pipe and the branch...

Embodiment 3

[0055] Such as figure 1 As shown, it is a nanofiber foam-based protein adsorption and separation device, and the nanofiber foam-based protein adsorption and separation device includes a perforated protective cover 1, and the perforated protective cover 1 is provided with a nanofiber foam-based protein adsorption material 4. The foam-based protein adsorption material 4 is provided with a liquid-collecting capillary network 2, and the liquid-collecting capillary network 2 is connected to the main infusion pipe 3, and the main infusion pipe 3 is connected to the liquid collection tank 6 through the pump 5.

[0056] The preparation method of the above-mentioned nanofiber foam-based protein adsorption and separation device, the specific steps are:

[0057] The first step: directly embed the liquid collection capillary network 2 in the nanofiber foam during the preparation of the nanofiber foam material; the liquid collection capillary network 2 includes the main pipe and the branch...

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Abstract

The invention provides a nanofiber foam-based protein adsorptive separation device and a preparation method thereof. The nanofiber foam-based protein adsorptive separation device is characterized by comprising an open pore protection cover, wherein the open pore protection cover is internally provided with a nanofiber foam-based protein adsorption material; a liquid collection capillary network is arranged in the nanofiber foam-based protein adsorption material; the liquid collection capillary network is connected with a main infusion tube; and the main infusion tube is connected with a liquid collection tank through a pump. The nanofiber foam-based protein adsorptive separation device has the advantages of simple preparation process, low production cost, convenience in operation and strong expandability; and the protein adsorption material has the advantages of high mass transfer rate, high adsorption rate, high adsorption capacity, high protein dissolution rate and good pressure resistant property and the proteins in the solution can be intensively adsorbed onto the material through a direct extraction manner to realize the batch efficient continuous separation and adsorption of the protein-containing solution; therefore, the nanofiber foam-based protein adsorptive separation device has wide application prospect in the fields of biological pharmacy and the like.

Description

technical field [0001] The invention belongs to the technical field of protein adsorption and separation, and in particular relates to a nanofiber foam-based protein adsorption and separation device capable of realizing rapid adsorption and separation of proteins and a preparation method thereof. Background technique [0002] Efficient separation and purification of proteins is one of the key technologies in modern drug analysis, analytical chemistry and life science research. The existing protein separation and purification methods mainly include: dialysis, ultrafiltration, density gradient centrifugation, and gel filtration based on protein molecular size differences; isoelectric point precipitation, salt dissolution and salting out methods based on protein solubility differences , low temperature organic solvent precipitation method; electrophoresis method based on the difference in the charge of the protein, ion exchange chromatography separation method; affinity chromat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/08B01D15/20B01J20/28C07K1/22
Inventor 丁彬傅秋霞葛建龙斯阳俞建勇
Owner DONGHUA UNIV
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