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Up-conversion luminescence-based chip detection system and reagent for gynecological tumor markers CA125, CA153, SCCA, HE4

A technology for CA125 and gynecological tumors, applied in the field of medical products, can solve the problems of lengthy and time-consuming conventional procedures, high cost, and not easy to carry

Inactive Publication Date: 2015-03-18
上海凯璟生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Typical immunoassay methods include enzyme-linked immunosorbent assay (elisa) and western blot (western blot). The routine procedures of immunoassay are tedious, time-consuming, difficult to carry and costly

Method used

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  • Up-conversion luminescence-based chip detection system and reagent for gynecological tumor markers CA125, CA153, SCCA, HE4
  • Up-conversion luminescence-based chip detection system and reagent for gynecological tumor markers CA125, CA153, SCCA, HE4
  • Up-conversion luminescence-based chip detection system and reagent for gynecological tumor markers CA125, CA153, SCCA, HE4

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Experimental program
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Effect test

Embodiment example 1

[0040] Implementation Case 1: Reaction Cup Detection System

[0041] 1, such as figure 1 Add a total of 100 microliters of UCP particle-labeled CA125, CA153, SCCA, and HE4 protein solutions (both at a concentration of 1 mg / ml) into the tube, and cover with Figure 5 The lid with a round hole in the middle is placed in a freeze dryer and freeze-dried at -50°C.

[0042] 2, such as Image 6 There are 12-20 spots of protein immobilized on black polystyrene, added in such as Image 6 Inside the concave hole, 0.1-0.5 μl per point. Point spacing 0.5-1mm.

[0043] 3. Coat the protein on the polystyrene sheet, and coat 4 kinds of protein mouse anti-human CA125, CA153, SCCA, HE4, so that the 4 kinds of protein are on a straight line parallel to the bottom edge of the cuvette, above the detection protein, Coated with rabbit anti-goat IgG (1mg / ml) as a quality control point. fixed in as Figure 4 On the handle rack. put in as figure 1 put into a constant temperature shaking shake...

Embodiment example 2

[0046] Implementation Case 2: Detection of Immunochromatographic Test Strips

[0047] 1. Coating: Dilute rabbit anti-goat IgG to 1mg / ml, as C-line coating solution, mix mouse anti-human CA125, CA153, SCCA, HE4 four monoclonal antibodies (both concentrations are 1mg / ml), as T Wire. The T-line coating solution and the C-line coating solution are coated on the nitrocellulose membrane by a film spraying machine, and dried to obtain a monoclonal antibody-coated test strip.

[0048] 2. Use UCP to mark the four antibodies CA125, CA153, SCCA, and HE4 for use.

[0049] 3. Preparation of the conjugation pad: soak the above-mentioned four kinds of antibodies labeled with UCP on the glass fiber pad, and bake in an oven at 37° C. for 2 hours. Cut into strips after drying.

[0050] 4. Assemble the test strip: attach the nitrocellulose membrane, absorbent paper, binding pad, and sample pad to the bottom plate.

[0051] 5. Add the sample to the sample pad. After 5-15 minutes, insert the t...

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Abstract

The invention relates to an up-conversion luminescence-based detection system for four gynecological tumor markers (CA125, CA153, SCCA, HE4), and the detection is performed by using a reaction cup detection system. The reaction cup detection system comprises a carrier plate, a reaction detection cup, and a fluorescence detector. The reaction detection cup is composed of a reaction detection cup and a test tube cover. A sample is dropwise added into the reaction detection cup for detection; after reaction for 5-15 min, the carrier plate is taken out and inserted into a slot of the fluorescence detector for value reading. The invention is characterized in that: the detection of four gynecological tumor markers CA125, CA153, SCCA, HE4 is carried out simultaneously by combining with an up-conversion luminescence detection method. The background value is small, and the measurement is rapid, accurate, and simple in operation. Data are transmitted to a doctor through wifi so as to obtain the detection results rapidly, and thus the purpose of rapid diagnosis is realized.

Description

technical field [0001] The invention relates to an immunological detection system and reagents for detecting four markers of gynecological tumors, CA125, CA153, SCCA and HE4. It is a device for detecting four markers of gynecological tumors by an up-conversion luminescence method, and belongs to the field of medical products. Background technique [0002] Breast cancer, ovarian cancer, endometrial cancer, cervical squamous cell carcinoma, cervical cancer and other gynecological tumor diseases are the main obstacles affecting the health of women. Cervical cancer is the most common tumor of the female reproductive tract. In recent years, the incidence rate has increased and the onset has become younger. Among gynecological cancers worldwide, cervical cancer is the main cause of death, with an incidence rate of 493,243 cases per year. The mortality rate is 273,505 cases / year, and the global mortality rate is 55%. Gynecological tumors have attracted widespread attention from al...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/533G01N33/574
CPCG01N33/57484G01N33/57411G01N33/57415G01N33/57442G01N33/57449G01N33/582
Inventor 罗朝领王微微茅柳娟肖建国王连会王亮马丽胡丽莉罗智允罗朝举
Owner 上海凯璟生物科技有限公司
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