Test paper for identifying and detecting virulent strain and low virulent strain of hog cholera virus

Abstract

The invention relates to livestock epidemic disease infection and immune identification and detection instruments, and in particular relates to a piece of test paper for identifying and detecting a virulent strain and a low virulent strain of hog cholera viruses. The test paper consists of a support plate, a sample pad, a gold mark pad, a detection membrane and a water absorbing pad, wherein a virulent infection detection line T1 '|', a low virulent infection detection line T2 '|' and a quality control line C '|' are arranged on the detection membrane. When the test paper is used, three red strips '|||' mean virulent virus infection of hog cholera viruses, two red strips '||' mean vaccine immunity of hog cholera viruses, and one red strip '|' means hog cholera viruse negative. The test paper is high in specificity, high in sensitivity, wide in reaction spectrum and simple, convenient and rapid to operate, can be used for detecting conventional low virulent vaccine strains and multiple epidemic virulent strains, can be widely used for identifying and detecting hog cholera virus infection and immune, and can be easily popularized and applied in production practice.

Description

technical field [0001] The invention relates to a detection device for identification and immunity of livestock epidemic diseases, in particular to a test paper for identification and detection of strong and weak swine fever virus. Background technique [0002] Classical swine fever (CSF) is a highly contagious disease characterized by high fever, hemorrhage and high mortality caused by classical swine fever virus (CSFV), the World Organization for Animal Health (OIE) It is included in the OIE Epidemic Disease List as an animal infectious disease that must be declared, and it is listed as a first-class animal epidemic disease in my country. my country has used hog cholera lapinized vaccine (HCLV) vaccine to prevent and control the large-scale epidemic of CSF. Sexual infection, so-called "atypical swine fever", "mild swine fever" and "venomous sow syndrome" have appeared, which are still one of the main infectious diseases that endanger the pig industry. CSFV belongs to the F...

AI Technical Summary

Problems solved by technology

[0003] At present, the prevention and control of swine fever in my country still adopts a strategy based on vaccine immunization. HCLV vaccine is a world-recognized excellent vaccine developed in the 1950s in my country. It has good safety, good immunogenicity, and stable genetic characteristics. It can be used simultaneously Inducing humoral immunity and cellular immunity can produce immune protection against different gene subgroups (1.1-3.4 subgroups). However, due to the lack of serological markers and supporting differential diagnosis methods, its large-scale application in my country makes it difficult to pass antibody detection. Difficult to distinguish between immune attenuated virus and wild virus infection, which is not conducive to the purification of CSF

With the completion of the complete gene sequences of CSFV wild strains and vaccine strains from different sources and regions and the development of molecular biology detection technology, scholars at home and abroad have conducted a lot of research on the identification and detection of CSFV. Strain characteristic sites, using 5'-UTR, NS5B or 3'-UTR conserved sequences as detection targets, respectively designed strong and weak strain-specific primers or probes, and established a method for distinguishing CSFV wild virus and rabbit attenuated vaccine Multiplex or nested RT-PCR, dual or composite fluorescent quantitative RT-PCR and other molecular identification detection methods have high sensitivity and specificity, but these detection methods need to rely on PCR machines or fluorescent quantitative PCR machines, and there are operating problems. The shortcomings of cumbersome, time-consuming, and high testing costs limit its application in epidemiological investigations and veterinary clinics

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