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SNP marker associated with pig growth rate, and applications thereof

A technology of rapid growth and rapid growth, applied in the field of genetic breeding of pig molecular markers, can solve the problems of inaccurate and convenient detection methods, low genotype effects, etc., and achieve the effects of shortening the generation interval, speeding up the breeding process, and rapid detection methods

Active Publication Date: 2015-03-11
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, the polymorphic sites of the pig LXRα gene that have been reported so far either have low genotype effects, or the detection methods are not accurate and convenient enough

Method used

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  • SNP marker associated with pig growth rate, and applications thereof
  • SNP marker associated with pig growth rate, and applications thereof
  • SNP marker associated with pig growth rate, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening of SNP sites of pig LXRα gene

[0026] Select the Chinese local breed "Tibetan pig" (collected from the teaching practice ranch of Tibet Agriculture and Animal Husbandry College),

[0027] Ear tissue samples of “Southern Diannan Small-eared Pig” (collected from Diannan Small-eared Pig Resource Protection Farm, Xishuangbanna Prefecture, Yunnan Province) and the introduced breed “Dark Yorkshire Pig” (collected from Anhui Kexin Pig Breeding Co., Ltd.) were tested with phenol / chloroform extraction of genomic DNA from tissues.

[0028] Download the porcine LXRα gene sequence (GenBank accession number: NC_010444.3) from NCBI, and use the software Primer Premier 5.0 to design the following primers according to the DNA sequence of the LXRα gene downloaded from NCBI:

[0029] Forward primer F: 5'-ATCTCTTCCTTGTCTTTACCC-3'

[0030] Reverse primer R: 5'-CAATCCCTTTGTGATCTCAG-3'

[0031] Use the above primers to perform PCR amplification on the genomic DNA of T...

Embodiment 2

[0034] Example 2 Establishment of PCR-RFLP Detection Method for Exon5-A201C Site of Porcine LXRα Gene

[0035] In order to quickly and conveniently detect the polymorphism at Exon5-A201C of the porcine LXRα gene, the mutation at this site was found to be recognized by Taq I endonuclease (TCGA) through sequence restriction endonuclease analysis. For the position of the Exon5-A201C mutation site, according to the LXRα gene sequence (GenBank accession number: NC_010444.3), use the software Primer Premier 5.0 to design new amplification primers. The primer sequence is:

[0036] Forward primer F: 5'-AAGAAACTGAAGCGGCAAGAG-3'

[0037] Reverse primer R: 5'-ATCGCAGAGGTCTTTAGGAGG-3'

[0038] Use this pair of primers to amplify the genomic DNA of Tibetan pigs, Diannan small-eared pigs, and Yorkshire pigs by PCR. The PCR reaction system is 25 μL, including 2.5 μL of 10×PCR reaction buffer, 2.0 μL of 10 mmol / L dNTP mix, and 5 pmol / L 1 μL of each primer, 0.5 μL of Taq DNA polymerase (5U / μ...

Embodiment 3

[0040] Example 3 Polymorphism Detection of SNP Markers Related to Pig Growth Velocity in Different Pig Groups

[0041] Collect Tibetan pigs (Bujiangda County, Nyingchi, Tibet), Diannan small-eared pigs (Xishuangbanna, Yunnan), big Pulian black pigs (Shandong Jiaxiang), large Yorkshire pigs (Anhui Kexin Pig Breeding Co., Ltd.), Landrace pigs (Beijing Zhongshun Jingsheng) Breeding Co., Ltd.) and Duroc pig (Anhui Kexin Pig Breeding Co., Ltd.) ear tissue samples, and the pig individual genomic DNA samples were extracted by the phenol / chloroform method.

[0042] Using the PCR-RFLP technique established in Example 2, the PCR-Taq I-RFLP genotype of the LXRα gene Exon5-A201C of individual Tibetan pigs was determined, and the detection results are shown in Table 1.

[0043] Table 1 Polymorphism distribution of porcine LXRα gene Exon5-A201C site

[0044]

[0045] Tibetan pigs, Diannan small-eared pigs, and big Pu-faced pigs all belong to Chinese local pig breeds with slow growth rat...

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Abstract

The present invention provides a SNP marker associated with pig growth rate, and application thereof, wherein the SNP marker is located on the 201 bp position of the pig LXR[alpha] gene exon 5, and the growth rate of the pig with the base A at the position is significantly higher than the growth rate of the pig with the base C at the position. According to the present invention, the polymerase chain reaction (PCR) and the sequencing technology are adopted to screen the SNP marker associated with pig growth rate, the restriction fragment length polymorphism (RFLP) method is adopted to detect the genotype of the pig gene to be detected, and the breeding of the predominant variety of the pig with the rapid growth is performed according to the genotype so as to accelerate the pig breeding process.

Description

technical field [0001] The invention belongs to the field of genetic breeding of pig molecular markers, and in particular relates to a SNP marker related to pig growth speed and application thereof. Background technique [0002] Pig farming is the main body of my country's animal husbandry production, and its output value has always been the first in the total output value of animal husbandry for a long time. With the improvement of people's living standards, the demand for the quantity and quality of meat products continues to increase. How to breed pigs with fast growth and good meat flavor is a hot spot in current pig breeding work. [0003] my country is rich in pig breed resources, which provide good materials for pig breeding, and also provide good materials for the study of genes and molecular markers of important economic traits. Tibetan pig is a unique plateau pig breed in my country. It has a small body, thin skin, tender and delicious meat, rich flavor, delicate ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张浩张博徐艾诗商鹏王志秀
Owner CHINA AGRI UNIV
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