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Bacillus subtilis and application thereof in plant disease control

A technology for preventing and controlling Bacillus subtilis and plant diseases, applied in the field of microbial screening, can solve problems such as unstable disease prevention effect of Bacillus subtilis, and achieve strong bacteriostatic effect, obvious antagonistic effect and broad prospects.

Active Publication Date: 2015-03-11
山东蔚蓝绿源生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are abundant microbial species in the growth environment of plants, and there is great potential to develop beneficial bacillus, but the disease prevention effect of biocontrol bacillus is sometimes unstable, and the disease prevention effect of bacillus screened in different regions varies greatly, so the development of more Many beneficial bacillus are one of the key points in the development of biocontrol agents

Method used

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  • Bacillus subtilis and application thereof in plant disease control
  • Bacillus subtilis and application thereof in plant disease control
  • Bacillus subtilis and application thereof in plant disease control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 strain screening and isolation

[0015] 1. Preparation of soil sample dilution:

[0016] Retrieve the soil from the vegetable greenhouse in Yangcun, Beicheng, Haiyang, weigh 0.5g soil sample and dissolve it in 4.5ml sterile water to make a 1:10 soil solution, then draw 0.5ml soil solution from it and place it in 4.5ml sterile water to prepare Make a 1:100 soil solution, and by analogy with this method, prepare a 1:10 6 -10 7 soil dilution solution.

[0017] 2. Strain isolation and purification:

[0018] Take 0.1ml of 3-4 dilutions of appropriate concentration and evenly spread them on the nutrient agar medium; take them out after culturing at 37°C for 2 days, pick a single colony and continue to isolate and purify until the colonies grown on each plate have the same shape and color, That is, a single strain.

[0019] Using the above method, the applicant screened out a total of 12 strains of bacteria, which were respectively named as FX1, FX2, FX3, ... FX...

Embodiment 2

[0022] Example 2 Verification experiment on control effect of cucumber powdery mildew

[0023] 1. Preparation of bacterial solution

[0024] The four screened strains FX1, FX4, FX6 and FX10 were respectively inoculated in nutrient broth medium, cultured at 30°C for 2-3 days, and the viable bacteria content was 10 8 -10 9 cfu / ml bacterial solution.

[0025] 2. Experimental process

[0026] 1) Experimental group: four strains of FX1, FX4, FX6, and FX10 were used to spray cucumber leaves with the same amount; three treatment groups were set up for each strain, and each treatment group consisted of randomly selected two ridge cucumbers, a total of 100 plants, each Set guard lines between processing groups.

[0027] In the mid-growth period of cucumbers, the above-mentioned bacterial solution was diluted 500 times with water, and then evenly sprayed on the cucumber leaves of each treatment group; the interval between each spraying was 7 days, and it was used three times in a ro...

Embodiment 3

[0037] Example 3 Identification of FX1 strains

[0038] 1) Physiological and biochemical characteristics of the FX1 strain:

[0039] The colony is flat, the surface is rough and opaque, stained white, the spore size is 0.6-0.9×0.9-1.4μm, oval or columnar, mesozoic or near mesozoic; Gram reaction is positive, catalase reaction is positive, V.P reaction is positive, Positive for hydrolysis of starch, positive for liquefaction of gelatin, positive for decomposition of casein, growth temperature range 25-40°C, pH range 5-8.

[0040] 2) Molecular biology identification of FX1 strain:

[0041] The FX1 strains screened above were identified using molecular biology methods, their 16s rDNA sequences were measured, and blast comparisons were performed in the GenBank nucleic acid database. Combining the biological characteristics of the FX1 strain and the 16srDNA comparison results, the applicant confirmed that the FX1 strain was Bacillus subtilis, and named it Bacillus subtilis FX1. ...

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Abstract

The invention provides a bacillus subtilis and an application thereof in plant disease control, relates to the technical field of microorganism screening, and specifically provides a bacillus subtilis FX1 screened from the soil of a vegetable greenhouse, wherein the preservation number of the bacillus subtilis FX1 is CCTCC NO.M2014563. The strain has very strong antibacterial effect and can be widely applied to the plant disease control. The cucumber powdery mildew control efficiency of the strain is up to 95.5%, the strawberry powdery mildew control efficiency of the strain is up to 94.8%, and the grape brown spot control efficiency is up to 83.2%. Besides controlling the plant powdery mildew and brown spot, the bacillus subtilis FX1 further has remarkable antagonism to plant yellow rot and fruit rot caused by fusarium and rhizoctonia solani, the control efficiency is up to more than 80%, and the application prospect is wide.

Description

technical field [0001] The invention relates to the technical field of microbial screening, in particular to a bacillus subtilis and its application in plant disease control. Background technique [0002] With the continuous increase of plant species and the continuous diversification of plant cultivation methods, plant diseases are becoming more and more serious. However, traditional chemical control methods have many disadvantages, such as: the extensive use of chemical pesticides has caused serious environmental pollution, pesticide residues in agricultural products It is also becoming more and more serious, and the rate of bacterial resistance to antibiotics is getting faster and faster, which has a series of harmful effects on people's lives. In today's low-carbon economy, environmental protection and conservation-oriented society, seeking new technologies that can protect human life and increase production is very suitable for the development of today's society. [00...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
CPCA01N63/00C12N1/205C12R2001/125
Inventor 苑伟伟王鹏唐波朱彩彩甄丽刘鲁民
Owner 山东蔚蓝绿源生物科技有限公司
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