Compound bacterium preparation and application thereof

A compound bacteria and preparation technology, applied in the direction of application, bacteria, microorganisms, etc., can solve the problems of no anti-prawn virus disease strains, unstable effect, and few bacillus strains, so as to enhance the non-specific immune function of shrimp and reduce virus Infection, effect of promoting digestion

Active Publication Date: 2015-06-24
湖北宜水生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few Bacillus strains used in the production of prawn farming at present, and there are deficiencies such as mediocre biological properties, low number of viable bacteria, and unstable effects, especially the application of anti-prawn virus disease strains.

Method used

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  • Compound bacterium preparation and application thereof
  • Compound bacterium preparation and application thereof
  • Compound bacterium preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Screening of Bacillus licheniformis

[0022] The Bacillus lincheniformis LV0005 strain of the present invention is selected from the digestive tract of Litopenaeus vannamei. The collected healthy prawn samples were transported back to the laboratory in inflatable bags filled with seawater. Wipe the body surface of live shrimp with 75% alcohol on the aseptic operation table for disinfection, dissect the live shrimp by aseptic operation, and take out the intestinal tissue of the prawn. After washing the removed intestinal tissue with sterile PBS (pH=7.0) buffer solution for 3-4 times, grind it evenly with a sterile grinding rod. Afterwards, it was appropriately diluted with sterile PBS buffer, and 0.1 ml was taken and spread on the 2216E solid medium, and placed upside down in a constant temperature incubator at 28°C for 16h-20h. Observe the colony shape of the bacteria on the 2216E plate, number the bacteria with the same colony shape, and pick a single colo...

Embodiment 2

[0029] Example 2: Screening of Bacillus pumilus

[0030] The Bacillus pumilus LV012 strain of the present invention is screened from the digestive tract of Litopenaeus vannamei. The collected healthy prawn samples were transported back to the laboratory in inflatable bags filled with seawater. Wipe the body surface of live shrimp with 75% alcohol on the aseptic operation table for disinfection, dissect the live shrimp by aseptic operation, and take out the intestinal tissue of the prawn. After washing the removed intestinal tissue with sterile PBS (pH=7.0) buffer solution for 3-4 times, grind it evenly with a sterile grinding rod. Afterwards, it was appropriately diluted with sterile PBS buffer, and 0.1 ml was taken and spread on the 2216E solid medium, and placed upside down in a constant temperature incubator at 28°C for 16h-20h. Observe the colony shape of the bacteria on the 2216E plate, number the bacteria with the same colony shape, and pick a single colony of the domi...

Embodiment 3

[0039] The screening of embodiment 3 subtilis

[0040] The Bacillus subtilis LV023 strain of the present invention is selected from the digestive tract of Litopenaeus vannamei. The collected healthy prawn samples were transported back to the laboratory in inflatable bags filled with seawater. Wipe the body surface of live shrimp with 75% alcohol on the aseptic operation table for disinfection, dissect the live shrimp by aseptic operation, and take out the intestinal tissue of the prawn. After washing the removed intestinal tissue with sterile PBS (pH=7.0) buffer solution for 3-4 times, grind it evenly with a sterile grinding rod. Afterwards, it was appropriately diluted with sterile PBS buffer, and 0.1 ml was taken and spread on the 2216E solid medium, and placed upside down in a constant temperature incubator at 28°C for 16h-20h. Observe the colony shape of the bacteria on the 2216E plate, number the bacteria with the same colony shape, and pick a single colony of the domin...

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Abstract

The invention aims to provide a compound bacterium preparation and an application thereof. The compound bacterium preparation is a compound strain preparation which is derived from bacillus licheniformis, bacillus pumilus and bacillus subtilis in digestive tracts of prawns, wherein the bacterial strains are combined for use so that the immune level of cells and body fluid of the prawns can be enhanced; in particular, the anti-virus infection capability of the prawns can be improved. The compound bacterium preparation comprises bacillus licheniformis LV005 strains, bacillus pumilus LV012 strains and bacillus subtilis LV023 strains. The strains of the compound bacterium preparation are screened from the digestive tracts of the prawns, so that the compound bacterium preparation has the effects of improving composition and number of dominant bacterial communities of the digestive tracts of the prawns, forming and retaining good microecological balance of the digestive tracts, resisting adhesion and proliferation of viruses in the digestive tracts by competition for nutrients, competition for space or secretion of bacteriocin, improving non-specific immunity functions of the prawns by improving the activity of relevant immunoenzymes such as lysozyme and catalase of the prawns, and reducing the probability of virus infection.

Description

technical field [0001] The invention belongs to the technical field of screening and preparation of probiotics, and in particular relates to a compound bacteria preparation and its application. Background technique [0002] Human beings are faced with three major problems of population, resources and environment, and the development and utilization of the ocean has become the national strategy of coastal countries. With the sharp decline of marine fishing resources, mariculture has become the main measure generally adopted by the international community to meet the increasing protein demand of human beings. The development of sustainable aquaculture has become a topic of common concern in the world, and aquaculture products have been widely recognized and affirmed by the international community as a source of high-quality protein. As a major producer of aquatic products in the world, China's total aquaculture production accounts for more than 70% of the world's total output...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/18A23K1/16A23K1/175C12R1/10C12R1/08C12R1/125
CPCC12N1/20
Inventor 宋晓玲黄倢杨冰许华李玉宏
Owner 湖北宜水生物科技有限公司
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