Compound bacterium preparation and application thereof
A compound bacteria and preparation technology, applied in the direction of application, bacteria, microorganisms, etc., can solve the problems of no anti-prawn virus disease strains, unstable effect, and few bacillus strains, so as to enhance the non-specific immune function of shrimp and reduce virus Infection, effect of promoting digestion
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Embodiment 1
[0021] Example 1: Screening of Bacillus licheniformis
[0022] The Bacillus lincheniformis LV0005 strain of the present invention is selected from the digestive tract of Litopenaeus vannamei. The collected healthy prawn samples were transported back to the laboratory in inflatable bags filled with seawater. Wipe the body surface of live shrimp with 75% alcohol on the aseptic operation table for disinfection, dissect the live shrimp by aseptic operation, and take out the intestinal tissue of the prawn. After washing the removed intestinal tissue with sterile PBS (pH=7.0) buffer solution for 3-4 times, grind it evenly with a sterile grinding rod. Afterwards, it was appropriately diluted with sterile PBS buffer, and 0.1 ml was taken and spread on the 2216E solid medium, and placed upside down in a constant temperature incubator at 28°C for 16h-20h. Observe the colony shape of the bacteria on the 2216E plate, number the bacteria with the same colony shape, and pick a single colo...
Embodiment 2
[0029] Example 2: Screening of Bacillus pumilus
[0030] The Bacillus pumilus LV012 strain of the present invention is screened from the digestive tract of Litopenaeus vannamei. The collected healthy prawn samples were transported back to the laboratory in inflatable bags filled with seawater. Wipe the body surface of live shrimp with 75% alcohol on the aseptic operation table for disinfection, dissect the live shrimp by aseptic operation, and take out the intestinal tissue of the prawn. After washing the removed intestinal tissue with sterile PBS (pH=7.0) buffer solution for 3-4 times, grind it evenly with a sterile grinding rod. Afterwards, it was appropriately diluted with sterile PBS buffer, and 0.1 ml was taken and spread on the 2216E solid medium, and placed upside down in a constant temperature incubator at 28°C for 16h-20h. Observe the colony shape of the bacteria on the 2216E plate, number the bacteria with the same colony shape, and pick a single colony of the domi...
Embodiment 3
[0039] The screening of embodiment 3 subtilis
[0040] The Bacillus subtilis LV023 strain of the present invention is selected from the digestive tract of Litopenaeus vannamei. The collected healthy prawn samples were transported back to the laboratory in inflatable bags filled with seawater. Wipe the body surface of live shrimp with 75% alcohol on the aseptic operation table for disinfection, dissect the live shrimp by aseptic operation, and take out the intestinal tissue of the prawn. After washing the removed intestinal tissue with sterile PBS (pH=7.0) buffer solution for 3-4 times, grind it evenly with a sterile grinding rod. Afterwards, it was appropriately diluted with sterile PBS buffer, and 0.1 ml was taken and spread on the 2216E solid medium, and placed upside down in a constant temperature incubator at 28°C for 16h-20h. Observe the colony shape of the bacteria on the 2216E plate, number the bacteria with the same colony shape, and pick a single colony of the domin...
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