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Vaccine composition, and preparation method and application thereof

A vaccine composition and a technology for respiratory syndrome, applied in the field of veterinary biological products, can solve the problems of increased stress response of pigs, increased risk of infection, failure of immunization of pigs, etc., so as to reduce the cost of epidemic prevention and the effect of immunity. Excellent, high potency effect

Inactive Publication Date: 2015-01-21
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are PCV2 inactivated vaccines, PPV inactivated vaccines, PRRSV inactivated and live vaccines on the market, but there is no PCV2-PRRSV-PPV triple vaccine” product production and sales
This preventive method requires divided immunization, and at least two injections are required to prevent multisystem syndrome in weaned piglets and reproductive disorders in sows. The cost is high and the operation procedures are cumbersome, which increases the risk of infection and causes stress in the pig herd. The reaction is increased, and the use of different vaccines will also cause immune interference and lead to immune failure in some pig herds.

Method used

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  • Vaccine composition, and preparation method and application thereof
  • Vaccine composition, and preparation method and application thereof
  • Vaccine composition, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1, Porcine Circovirus, Porcine Japanese Encephalitis Virus and Porcine Parvovirus Triple Inactivated Vaccine Composition, Preparation and Inspection of Porcine Japanese Encephalitis Virus and Porcine Parvovirus Dual Vaccine

[0034] 1. The source of the virus strain

[0035] The PCV2 virus described in the triple vaccine of the present invention is the PCV2SH strain, which has been preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee. The preservation date is March 4, 2008, and the preservation number is CGMCC No2389.

[0036] The PRRSV strain described in the triple seedling of the present invention is the NVDC-JXA1 strain, which has been preserved in the General Microorganism Center of the China General Microorganism Culture Collection Management Committee. The preservation date: March 9, 2007, and the preservation number is CGMCC No1964.

[0037] The PPV strain described in the triple vaccine of the...

Embodiment 2

[0085] Embodiment 2, this study is to evaluate the immune efficacy of different antigen content PCV2-PRRSV-PPV combination vaccines and each virus single vaccine

[0086] 1. Test material

[0087] According to the method in embodiment 1, prepare PCV2-PRRSV-PPV triple vaccine (PCV2 antigen content is 10 6.0 TCID 50 / ml, PRRSV antigen content is 10 7.0 TCID 50 / ml, PPV antigen content is 10 6.0 TCID 50 / ml), recorded as vaccine L; PCV2-PRRSV-PPV triple vaccine (PCV2 antigen content is 10 6.5 TCID 50 / ml, PRRSV antigen content is 10 8.0 TCID 50 / ml, PPV antigen content is 10 7.0 TCID 50 / ml), recorded as vaccine M; PCV2-PRRSV-PPV triple vaccine (PCV2 antigen content is 10 7.0 TCID 50 / ml, PRRSV antigen content is 10 9.0 TCID 50 / ml, PPV antigen content is 10 8.0 TCID 50 / ml), recorded as vaccine H; low-dose PCV2-PRRSV-PPV triple vaccine (PCV2 antigen content is 5×10 5.5 TCID 50 / ml, PRRSV antigen content is 10 8.0 TCID 50 / ml, PPV antigen content is 10 7.0 TC...

Embodiment 3

[0115] Example 3, this study is to reproduce the sow reproductive disorder model under laboratory conditions

[0116] 1 Animal experiment design

[0117]Select 28 gilts and divide them into 7 groups with 4 gilts in each group. All sows were inoculated with 1ml each of PCV SH strain strong virus strain, PRRSV NVDC-JXA1 strong virus strain strain and PPV HN-2011 strong virus strain by intramuscular injection according to the experimental scheme described in Table 13, Observe the physiological state of the sows until farrowing. After delivery, observe whether there are symptoms of reproductive disorders such as miscarriage, stillbirth, mummified fetus, or fetal reabsorption. The presence or absence of PCV, PRRSV and PPV viruses in the internal organs of stillborn fetuses was detected; blood was collected from surviving fetuses to separate serum, and the presence of PCV, PRRSV and PPV antibodies was determined.

[0118] Table 13 Experimental scheme for replication of sow reprod...

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Abstract

The invention provides a vaccine composition comprising an immune amount of a porcine circovirus type 2 antigen, an immune amount of porcine reproductive and respiratory syndrome virus antigen, a porcine parvovirus antigen and a carrier acceptable in veterinary medicine. Antigens in the vaccine composition have no mutual interference on each other, even show some immune synergism; the composition causes small stress after immunization, is more safe, has immune effect better than that of a single vaccine, avoids the stress response caused by multiple inoculation, simplifies the complex immune procedure, and reduces the cost of epidemic prevention.

Description

technical field [0001] The invention relates to a triple vaccine and belongs to the field of veterinary biological products. Background technique [0002] Porcine circovirus disease or porcine circovirus-associated disease is an immunosuppressive disease mainly caused by porcine circovirus type 2 (Porcine circovirus2, PCV2), manifested as post-weaning piglet multisystem failure syndrome, porcine dermatitis nephropathy Syndrome, porcine respiratory disease syndrome, reproductive failure, piglet myocarditis, hyperplastic and necrotizing pneumonia and central nervous system diseases, etc. Porcine circovirus is a new pathogen that seriously harms the pig industry in recent years. It is the smallest DNA virus discovered so far. It has strong resistance to the external environment. Once the pigs are infected with porcine circovirus It is very difficult to eradicate. [0003] Porcine reproductive and respiratory syndrome, commonly known as porcine blue-ear disease, was first repo...

Claims

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Application Information

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IPC IPC(8): A61K39/23A61K39/12A61P31/20A61P31/14
Inventor 张许科孙进忠田克恭
Owner PU LIKE BIO ENG
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