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Preparation and application of auto-platelet-rich plasma thixotropic separating gel

A technology for platelet-rich plasma and gel separation, applied in the fields of polymer chemistry and biomedicine, can solve the problems of cumbersome process, affecting the effective acquisition of growth factors, platelet activation, etc., and achieve improved operation efficiency, excellent thixotropy and biocompatibility Sex, the effect of effective separation

Active Publication Date: 2015-01-07
广东龙帆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is to prepare PRP by centrifugation in an open system and transfer it through multiple containers. The process is cumbersome and easily contaminated by the outside world; the concentrations and activities of platelets and growth factors in different centrifugation times, centrifugal force and centrifugation time are also different; Moreover, the manual suction method is easy to activate platelets, which affects the effective acquisition of growth factors.
Therefore, traditional PRP preparation methods cannot ensure the safe and effective extraction of PRP, thus affecting the development and application of autologous PRP in the field of biomedicine.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Put 100g propyl acrylate, 2.0g hydroxyethyl acrylate, 6.0g nano-silicon dioxide and 1.0g silanization active agent into a 250ml four-necked bottle, install a thermometer, a mechanical stirrer, a distillation head and a condensation collection device, and import High-purity nitrogen, turn on the condensate, and start to slowly heat to 60~80°C. The reaction was stirred for 1 hour under nitrogen protection. Then add 0.2g of photoinitiator Irgacure 2595, use ultraviolet light (365nm, 30mWcm -2 ) for 3 minutes to react to prepare a thixotropic composite polymer-based glue. Then blend with 2.0g dioctyl phthalate and 0.6g span80, vacuum defoam, fill in the bottom of the vacuum centrifuge tube, and irradiate with cobalt-60 gamma rays (30kGy) to prepare a thixotropic composite polymer gel. glue.

[0024] The apparent viscosity of the gel is 1.885×10 5 mPa. s (tested by rotational viscometer, test temperature 25°C, speed 0.3rpm), thixotropic index 3.61, specific gravity 1.13...

Embodiment 2

[0026] Put 100g propyl acrylate, 4.0g hydroxyethyl acrylate, 4.0g nano-silicon dioxide and 0.6g silanization active agent into a 250ml four-necked bottle, install a thermometer, a mechanical stirrer, a distillation head and a condensation collection device, and import High-purity nitrogen, turn on the condensate, and start to slowly heat to 70~90°C. The reaction was stirred for 1.5 hours under nitrogen protection. Then add 0.3g of photoinitiator Irgacure 2595, and use ultraviolet light (365nm, 30mWcm -2 ) for 5 minutes to react to prepare a thixotropic composite polymer-based glue. It was then blended with 3.5 g dioctyl phthalate and 0.8 g span80. Fill in the bottom of the vacuum centrifuge tube, defoam in vacuum, and irradiate with cobalt-60 gamma rays (20kGy) to prepare a thixotropic composite polymer gel.

[0027] The apparent viscosity of the gel is 1.045×10 5 mPa. s (tested by rotational viscometer, temperature 25°C, speed 0.3rpm), thixotropic index 3.45, specific gr...

Embodiment 3

[0029] Put 100g propyl acrylate, 3.0g hydroxyethyl acrylate, 20g isoamyl acrylate, 7.5g nano silicon dioxide and 1.1g silanization active agent into a 250ml four-necked bottle, install a thermometer, a mechanical stirrer, a distillation head And the condensate collection device, introduce high-purity nitrogen, turn on the condensate, and start to slowly heat to 80~90°C. The reaction was stirred for 2 hours under nitrogen protection. Then add 0.3g of photoinitiator Irgacure 2595, and use ultraviolet light (365nm, 30mWcm -2 ) for 5 minutes to react to prepare a thixotropic composite polymer-based glue. It was then blended with 5.0 g dioctyl phthalate and 0.6 g span80. The thixotropic composite polymer gel was prepared by vacuum defoaming and cobalt-60γ-ray irradiation (25kGy).

[0030] The apparent viscosity of the obtained gel was 1.132×10 5mPa. s (temperature 25°C, speed 0.3rpm), thixotropic index 3.70, specific gravity 1.10. Take about 2 g of it and fill it in the botto...

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Abstract

The invention relates to preparation and application of auto-platelet-rich plasma thixotropic separating gel, belonging to the fields of high polymer chemistry and biomedicine. The preparation method comprises the following steps: by using acrylate monomer, nano silicon dioxide and silanization activator as raw materials, stirring and heating to react at 60-140 DEG C for 1-4 hours in a nitrogen protective atmosphere; and adding a photoinitiator, irradiating with ultraviolet light to obtain a thixotropic composite high-polymer-base adhesive, blending the thixotropic composite high-polymer-base adhesive with dioctyl phthalate and Span 80, carrying out vacuum degassing and irradiating with cobalt-60 gamma rays. The thixotropic separating gel has excellent thixotropy, biocompatibility and stability, and is suitable for safely, simply and completely extracting auto-platelet-rich plasma and other bioactive substances in biomedicine.

Description

technical field [0001] The invention relates to the preparation and application of autologous platelet-rich plasma thixotropic separation gel, belonging to the fields of polymer chemistry and biomedicine. Background technique [0002] Autologous platelet-rich plasma (Platelet-rich plasma, PRP) is the platelet concentrate produced by the separation of autologous blood. Autologous PRP is derived from the autologous body and has good safety performance. A large number of clinical studies have reported that PRP contains a large number of various platelet-derived cell growth factors, which can promote the repair of various tissues, and can promote the repair of bone and soft tissue. The promotion of bone defect repair by PRP may be mainly based on the following two mechanisms: one is that PRP is activated into platelet-rich gel (Platelet-rich gel, PRG) under the action of an activator, which contains a large amount of fibrinogen, which can seal the wound and promote tissue re...

Claims

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Application Information

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IPC IPC(8): C08J3/28C08J3/075C08L33/08C08F220/18C08F220/28C08F2/48C08K3/36
Inventor 吴建国杨小钢邓兆群谭秋萍
Owner 广东龙帆生物科技有限公司
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