A DNA vaccine containing Newcastle disease ag@sio 2 Preparation method of nanoparticles

A DNA vaccine and nanoparticle technology, applied in the field of preparation of Newcastle disease nanoparticle DNA vaccine, can solve the problems of high price and complicated preparation method, and achieve the effects of good stability, broad application prospects and strong thermal stability.

Active Publication Date: 2016-09-21
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] DNA vaccine vectors are divided into two categories: viral and non-viral vectors. Viral vector-mediated gene transfection efficiency is high, but it has potential immunogenicity and carcinogenicity, and the preparation method is complicated and expensive.

Method used

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  • A DNA vaccine containing Newcastle disease ag@sio <sub>2</sub> Preparation method of nanoparticles
  • A DNA vaccine containing Newcastle disease ag@sio <sub>2</sub> Preparation method of nanoparticles
  • A DNA vaccine containing Newcastle disease ag@sio <sub>2</sub> Preparation method of nanoparticles

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Experimental program
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specific Embodiment approach 1

[0022] Specific implementation mode 1: Ag@SiO, a DNA vaccine containing Newcastle disease in this implementation mode 2 The preparation method of nanoparticle, it is prepared according to the following steps:

[0023] 1. Ag@SiO2 2 Amination of core-shell nanoparticles: take 1.0-2.0 mg of Ag@SiO 2 Put the core-shell nanoparticles in a centrifuge tube, add 7mL of absolute ethanol, then add 20μL of 3-aminopropyltriethoxysilane and shake at room temperature for 3~5h; then centrifuge at 10000r / min~12000r / min 5min, suspend with 5mL of absolute ethanol, centrifuge again, collect the precipitate; repeat the centrifugation and suspension operation three times, collect the precipitate, dry the precipitate at room temperature, suspend with PBS buffer, ultrasonically disperse, and prepare a concentration of 1.5mg / mL of Ag@SiO 2 Core-shell nanoparticle suspension;

[0024] 2. Mix Ag@SiO with a mass ratio of 15:1 2 The core-shell nanoparticle suspension is mixed with plasmid DNA, and ...

specific Embodiment approach 2

[0025] Embodiment 2: This embodiment is different from Embodiment 1 in that: the pH of the PBS buffer solution in Step 1 is 7.4. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0026] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the plasmid in step 2 is pVAX I-optiF. Others are the same as in the first or second embodiment.

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Abstract

A Newcastle Disease DNA Vaccine Ag@SiO 2 A method for the preparation of nanoparticles, which involves the use of Ag@SiO 2 The preparation method of the Newcastle disease nanoparticle DNA vaccine as a carrier. Method: 1. Ag@SiO 2 Amination of core-shell nanoparticles, followed by washing with absolute ethanol, weighing after drying, and preparing a nanoparticle suspension with a concentration of 1.5 mg / mL; 2. Amination of Ag@SiO 2 The core-shell nanoparticles were combined with the plasmid (pVAX Ⅰ ‑optiF) at a mass ratio of 15:1, and left at room temperature for 0.5 h; 3. After vacuum freeze-drying, Ag@SiO 2 Newcastle disease nanoparticles as carrier. The present invention uses Ag@SiO 2 The particle size of the Newcastle disease nanoparticle used as the carrier is easy to control, and the particle size distribution is uniform; it has good histocompatibility and bioadhesion, can resist the digestion of various enzymes in the body, has low drug side effects, and has a long sustained release time. High utilization and easy scale production.

Description

technical field [0001] The present invention relates to Ag@SiO 2 The preparation method of the Newcastle disease nanoparticle DNA vaccine as a carrier. Background technique [0002] Newcastle disease (New Castle-disease, ND) is an acute and highly contagious poultry infectious disease caused by New Castle-disease virus (NDV), which belongs to the category A disease stipulated by OIE. Affected chickens showed symptoms such as dyspnea, diarrhea, nervous dysfunction, mucosal and serosal hemorrhage. The prevalence of the disease in different degrees in our country has seriously perplexed the development of the chicken industry and caused huge economic losses. Chickens are the most susceptible to Newcastle disease virus, especially chicks. At present, the basic measure to control ND is vaccination. Commonly used Newcastle disease vaccines are attenuated live vaccines and inactivated vaccines, but there are still some limitations in the practical application of the two. ND ina...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K39/17A61K47/04A61P31/14
Inventor 赵凯王晓华
Owner HEILONGJIANG UNIV
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