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SiRNA for targeted inhibition of Mus81 gene expression, siRNA plasmid, interfering lentivirus and construction method and application thereof

A technology of gene expression and lentivirus, which is applied in the field of genetic engineering, can solve the problems of no targeted inhibition application, achieve comprehensive therapeutic effects, inhibit growth and proliferation, and improve chemosensitivity

Inactive Publication Date: 2014-11-26
吴帆
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The realization of the RNAi gene silencing function depends on the selection of siRNA sequences and the construction of high-efficiency expression vectors. At present, there are no siRNAs, siRNA plasmids, interfering lentiviruses, and their use in the preparation of drugs for the treatment of liver cancer in the prior art. application

Method used

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  • SiRNA for targeted inhibition of Mus81 gene expression, siRNA plasmid, interfering lentivirus and construction method and application thereof
  • SiRNA for targeted inhibition of Mus81 gene expression, siRNA plasmid, interfering lentivirus and construction method and application thereof
  • SiRNA for targeted inhibition of Mus81 gene expression, siRNA plasmid, interfering lentivirus and construction method and application thereof

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Embodiment Construction

[0036] Figure 1 to Figure 13 Shown are examples of siRNA, siRNA plasmid, interfering lentivirus and its construction method and application for targeting and inhibiting Mus81 gene expression of the present invention. By designing the siRNA sequence of the human Mus81 gene and constructing the corresponding siRNA plasmid and interfering lentivirus, The method of lentivirus-mediated siRNA is used to specifically inhibit the expression of Mus81 gene in liver cancer cells, so as to realize the effects of inhibiting the growth and proliferation of liver cancer cells, promoting their apoptosis and improving their chemosensitivity.

[0037] 1. Design the siRNA sequence targeting to inhibit the expression of Mus81 gene

[0038] Firstly, the sequence of the human Mus81 gene (BC009999) was obtained from Genebank, and then three siRNA sequences targeting the Mus81 gene were designed using the design software Genechem of Shanghai Jikai Gene Chemical Technology Co., Ltd., and the siRNA se...

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Abstract

The invention discloses siRNA for targeted inhibition of Mus81 gene expression, siRNA plasmid, interfering lentivirus and a construction method and application thereof; a siRNA sequence of human Mus81 gene is designed, the corresponding siRNA plasmid and interfering lentivirus are constructed, lentivirus mediation siRNA method is used for specific inhibition of Mus81 gene expression in hepatoma carcinoma cells so as to realize the effects of inhibition of hepatoma carcinoma cell growth proliferation, promotion of hepatoma carcinoma cell apoptosis and improvement of hepatoma carcinoma cell chemosensitivity, and other various aspects, the Mus81 gene which is a very effective drug target for treatment of liver cancer is provided, and the siRNA has great significance to preparation of Mus81 gene-targeted treatment drugs of the liver cancer.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to siRNA, siRNA plasmid, interfering lentivirus and its construction method for targeting and inhibiting the expression of Mus81 gene, and its application in the preparation of drugs for treating liver cancer. Background technique [0002] The Mus81 gene belongs to the XPF endonuclease superfamily. It was first identified in yeast by Interthal H et al. in 2000. Because the yeast knocked out of this gene is more sensitive to ethanesulfonate and ultraviolet rays, Interthal H et al. for "Ethane Sulfonate and UV Sensitive to Independent Sequence No. 81" ( M MS and U V s sensitive isolate number 81 , Mus81). Chen XB et al subsequently identified the human Mus81 gene in 2001, which is located on the long arm of human chromosome 11 (11q13.1), contains 16 exons, and the encoded Mus81 protein has two DNA molecules Combined helix-hairpin-helix structure with a typical XPF-lik...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/867C12N15/66A61K48/00A61P35/00A61P1/16
Inventor 吴帆刘建伟戴丽冰
Owner 吴帆
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