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Gradual cooling auxiliary enzymatic method for glycerolysis preparation of lard diglyceride

A technology of gradual cooling and diglyceride, which is applied in the field of gradual cooling to assist the preparation of diglyceride, which can solve the problems of difficult absorption, high heat, and high melting point, and achieve the effect of easy separation

Inactive Publication Date: 2014-11-19
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] my country is rich in lard resources, but its high melting point makes it difficult to digest and absorb in the human body. At the same time, the main fatty acids composed are long-chain fatty acids, which provide high calories, so its processing and utilization degree is low

Method used

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  • Gradual cooling auxiliary enzymatic method for glycerolysis preparation of lard diglyceride

Examples

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Effect test

Embodiment 1

[0017] Mix lard and glycerin with a molar ratio of 1:1 in a round bottom flask, dehydrate for 30min under the conditions of vacuum degree -50.0kpa and heating temperature of 82°C, transfer the dehydrated mixture to the Erlenmeyer flask, add Lipozyme RM IM with 10% oil weight was reacted at a magnetic stirring speed of 400r / min. The initial reaction temperature was 65°C. After 2 hours of reaction, the temperature was adjusted to 55°C, reacted for 2 hours, and then adjusted to 40°C for 6 hours. After the reaction, the lipase was removed by filtration to obtain a glycerol hydrolyzate of lard. The content of DAG in the product was analyzed by high performance liquid chromatography to be 41.81%, and the content of 1,3-DAG was 26.52%.

Embodiment 2

[0019] Mix lard and glycerin with a molar ratio of 1:1 in a round bottom flask, dehydrate for 30 minutes under the conditions of vacuum degree -50kpa and heating temperature of 82°C, transfer the dehydrated mixture to a conical flask, add oil 14% Lipozyme RM IM was reacted at a magnetic stirring speed of 400r / min. The initial reaction temperature was 65°C. After 2 hours of reaction, the temperature was adjusted to 55°C, reacted for 2 hours, and then adjusted to 40°C for 6 hours. After the reaction, the lipase was removed by filtration to obtain a glycerol hydrolyzate of lard. The content of DAG in the product was analyzed by high-performance liquid chromatography to be 54.04%, and the content of 1,3-DAG was 39.68%.

Embodiment 3

[0021] Mix lard and glycerin with a molar ratio of 2:1 in a round bottom flask, dehydrate for 30 minutes under the conditions of vacuum degree -50kpa and heating temperature of 82°C, transfer the dehydrated mixture to a conical flask, add oil 14% Lipozyme RM IM was reacted at a magnetic stirring speed of 400r / min. The initial reaction temperature was 65°C. After 2 hours of reaction, the temperature was adjusted to 55°C, reacted for 2 hours, and then adjusted to 40°C for 6 hours. After the reaction, the lipase was removed by filtration to obtain a glycerol hydrolyzate of lard. The content of DAG in the product was analyzed by high-performance liquid chromatography to be 51.27%, and the content of 1,3-DAG was 38.28%.

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Abstract

A gradual cooling auxiliary enzymatic method for glycerolysis preparation of lard diglyceride belongs to the field of deep processing of lard. The method comprises the steps as follows: 1, removing water from raw materials of lard and glycerin in a molar ratio of 1-3:1 under vacuum and heating conditions; 2, adding a catalyst 1,3 specific lipase LipozymeRM IM in the weight 6-16% of the lard, and gradually cooling with magnetic stirring, reacting at 60-70 DEG C for 2-4 h, reacting at 50-60 DEG C for 2-4 h, reacting at 35-50 DEG C for 4-20 h; and 3, after the reaction, taking out the sample, and filtering to remove the lipase, so as to obtain the lard diglyceride. The invention uses lard as the raw material and employs the gradual cooling auxiliary enzymatic method for glycerolysis preparation of lard diglyceride, so as to make full utilization of the cheap lard resource; at the same time, the invention has the advantages of no organic solvent residual and easy separation of product and enzyme, and lays foundation for constructing processing technology of healthful diacylglycerol oil.

Description

technical field [0001] The invention belongs to the field of deep processing of lard, and relates to a process for assisting the preparation of diglyceride by gradually lowering the temperature while glycerolizing lard with lipase in a solvent-free system. Background technique [0002] Diacylglycerol (DAG) is the esterification product of two hydroxyl groups of glycerol and fatty acids, and exists in natural animal and vegetable oils as trace components. There are two isomers of 1,3-DAG and 1,2 (or 2,3)-DAG in diglyceride, and it mainly exists in the form of 1,3-DAG in most natural oils and artificial diglycerides. Diglyceride-rich oils and triglyceride (TAG) oils have similar taste, odor, color, cooking characteristics and have certain stability to heat and oxygen. But the metabolic mechanism of the two in the body is different. There are two long-chain fatty acids in the molecular structure of diglyceride, which is highly lipophilic. At the same time, it also contains a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
Inventor 孔保华刁小琴赵欣欣陈倩
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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