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Cordyceps sinensis mycelium fermentation production method

A technology for fermentation of Cordyceps sinensis fungus and mycelium, which is applied in the fields of botanical equipment and methods, horticulture, and application, can solve the problems of different screening cycles, different formulas and cultivation conditions, and achieves a solution to the decline of seed performance and good screening effect. Effect

Active Publication Date: 2014-11-19
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2. The screening cycle is different
2. Different formulations and culture conditions

Method used

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  • Cordyceps sinensis mycelium fermentation production method
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  • Cordyceps sinensis mycelium fermentation production method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Cordyceps sinensis mycelium liquid state fermentation production

[0035] Materials and instructions used: The newly isolated strain of Cordyceps sinensis (mother species) was identified as Cordyceps sinensis by molecular biology, and the number was DC-0; the 10th generation strain (number DC-10) was obtained by subculture of the strain based on the mother species. , the 15th generation strain (coded as DC-15), the 20th generation strain (numbered as DC-20), the 30th generation strain (numbered as DC-30);

[0036] Silkworm chrysalis or tussah silkworm chrysalis: commercially available fresh live silkworm chrysalis or tussah silkworm chrysalis for the preparation of interstitial fluid.

[0037] Preparation of Cordyceps fungus screening medium: solid substrate (10-40 parts of rice, 10-30 parts of corn flour, 15-35 parts of wheat, 10-30 parts of sorghum, 5-15 parts of oat flour, 5-15 parts of bran) With nutrient solution (tissue fluid of tussah silkworm chry...

Embodiment 2

[0060] Example 2: Parallel verification test of bacterial strain comparison before screening and after screening

[0061] The first set of parallel experiments:

[0062] Adopt the DC-0 bacterial classification identical with embodiment 1; Bacterial strain DC-15, DC-20, DC-30 in table 1 are screened respectively, screening method is as follows:

[0063] Wherein, the preparation of Cordyceps sinensis screening medium: solid substrate (10 parts of rice, 30 parts of corn flour, 15 parts of wheat, 30 parts of sorghum, 15 parts of oat flour, 5 parts of bran) and nutrient solution (50 g / L of tussah silkworm chrysalis tissue fluid) , glucose 10g / L, sucrose 10g / L, peptone 20g / L, yeast extract 5g / L, compound amino acid powder 5g / L, potassium dihydrogen phosphate 0.1g / L, dipotassium hydrogen phosphate 0.1g / L, magnesium sulfate 0.1 g / L, vitamin B 1 5mg / L, vitamin B 2 5mg / L, vitamin B 6 10mg / L) according to the ratio of 1:1.4 preparation.

[0064] Other operating methods are the sa...

Embodiment 3

[0077] Example 3 Application of screening method in strain subculture

[0078] Prepare the special medium for liquid fermentation of Cordyceps sinensis: 20 grams of glucose, 30 grams of silkworm chrysalis powder, 30 grams of corn, 50 grams of pure milk, 2 grams of glutamic acid, 2 grams of glycine, 15 grams of peptone, 10 grams of fish meal, 15 grams of yeast powder, Vitamin B1 20mg, Vitamin B2 20mg, Dipotassium Hydrogen Phosphate 0.4g, Potassium Dihydrogen Phosphate 0.4g, Magnesium Sulfate 0.2g, Tenebrio Molitor Oil 3g.

[0079] Put the silkworm chrysalis powder and corn into the cooking pot, add water and cook twice, 20 minutes each time, filter the gauze and take the supernatant into the container, then add other ingredients, heat to dissolve, and add water to 1000ml.

[0080] Using the DC-0 parent species in Example 1, the initial pH is 5.5-6.5, the inoculum size is 10%, and the Cordyceps sinensis strain is inoculated in the medium, and the liquid volume of the shake flask...

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Abstract

The invention provides a cordyceps sinensis mycelium fermentation production method. The method includes the steps of preparation of a special culture medium for cordyceps sinensis mycelium liquid state fermentation, optimization of a mycelium provenance, fermentation of a strain in the culture medium and the like, wherein optimization of the mycelium provenance includes the procedures that cordyceps sinensis strain mycelia obtained after more than ten times of artificial passage are inoculated to the artificial solid culture medium, superior fruit bodies are selected, and a preferred China hirsutella rhossiliensis strain is separated from the fruit bodies. The cordyceps sinensis mycelium fermentation production method has the advantages that through strain selection, the cordyceps sinensis strain with strain character advantages is obtained, the screening effect is good, the problem of strain character recession can be effectively solved, and meanwhile the method also can serve as a China hirsutella rhossiliensis strain passage preservation method.

Description

technical field [0001] The invention relates to a strain cultivation method of Cordyceps sinensis, in particular to a fermentation production method of Cordyceps sinensis mycelium, and the invention belongs to the field of medicine and health food. Background technique [0002] Cordyceps sinensis (Ophiocordyceps sinensis (Berk.) G.H.Sung et al.) belongs to Ascomycota, Hypocreales, Ophiocordycipitaceae, and Ophiocordyceps in taxonomy. Cordyceps sinensis is a rare and valuable Chinese medicinal material that is a special product in my country. It is a complex of insects and fungi formed by parasitizing Cordyceps sinensis in the larvae of insects of the genus Bata. The history of the use of Cordyceps sinensis was first recorded in "Compendium of Materia Medica Supplements". It is said that it has "the effects of nourishing the lungs and kidneys, stopping bleeding and resolving phlegm". Chronic bronchitis, hyperlipidemia and sexual dysfunction and other diseases have significan...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 贺宗毅陈仕江贺元川张德利鲁增辉李卿
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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