Method for simultaneous determination of contents of 6 kinds of sedative-hypnotic drugs in serum by UHPLC-CAD technology

Abstract

The invention relates to a method for simultaneous determination of the contents of 6 kinds of sedative-hypnotic drugs in serum by a UHPLC-CAD combined technology, wherein the 6 kinds of sedative-hypnotic drugs are diazepam, lorazepam, alprazolam, estazolam, clonazepam and zolpidem tartrate. The method is characterized by including the following steps: 1) reference substance preparation, 2) internal standard solution preparation, 3) sample solution preparation, 4) mobile phase solution preparation, 5) chromatographic condition setting, 6) electrospray detector condition optimization, 7) sample determination, 8) gradient-concentration reference substance solution preparation, 9) standard curve preparation, and 10) data calculation and analysis. The method is advanced, has good clinical application prospects and effects, can rationally use drugs for clinic, increases drug curative effects, lowers toxic and side effects and drug costs, and plays a positive role.

Description

Technical field [0001] The invention relates to a detection method that belongs to the field of medicines, in particular to a detection method for simultaneously determining six sedative and hypnotic drugs in serum by adopting a high-performance liquid phase-electrospray detection combined technology. Background technique [0002] In modern social life, due to fierce competition in work and tight life rhythm, various external stimulus factors affect people’s emotions, causing insomnia and tension, and then developing into various psychological and physical problems such as anxiety and depression, which have caused huge consequences for family and society. pressure. A foreign survey showed that 50%-60% of adults suffer from sleep disorders of varying degrees, and the incidence of insomnia in my country is as high as 40%. As the first-generation sedative and hypnotics, barbiturates have been basically replaced by other related drugs in recent years due to their adverse reactions s...

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Problems solved by technology

According to the literature search, there are currently no literature reports or patent publications on the method for measuring the content of six sedative-hypnotic drugs (including the second-generation and third-generation drugs) in serum by UHPLC-CAD method, and the application of blood drug monitoring in psychiatric patients.

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