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Bacillus thuringiensis for preventing and controlling bradysia odoriphaga, and application of bacillus thuringiensis

The technology of Bacillus aureus and oyster mosquito is applied in the field of biological control, which can solve the problems of excessive pesticide residues in leek, difficult prevention and control, and environmental pollution, and achieve the effects of eliminating poisonous leek incidents, ensuring food safety, and having a simple preparation method.

Active Publication Date: 2014-10-15
WUHAN KERNEL BIO-TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the shortcomings of physical control methods such as cumbersome operation and great influence on the environment, chemical methods such as pesticide root irrigation are mainly used for control in production. Excessive or even illegal use of prohibited high-toxic pesticides for prevention and control has resulted in excessive pesticide residues, polluted the environment, endangered human health, and killed beneficial birds. The vicious cycle of birds and pests becoming rampant again cannot achieve effective control of pest populations. At the same time, it causes leeks to exceed the standard of pesticide residues, threatening the health of consumers
In recent years, poisonous leek incidents have occurred frequently, such as: "Xianghe poisonous leek incident in Beijing" in April 2004, "Qingdao poisonous leek incident" in April 2010, and "poisonous leek incident" in Nanyang, Henan Province in March 2011. Continuous exposure of poisonous leeks The incident made consumers "disgusted" and seriously affected the economic interests of vegetable farmers and the food safety of consumers

Method used

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  • Bacillus thuringiensis for preventing and controlling bradysia odoriphaga, and application of bacillus thuringiensis
  • Bacillus thuringiensis for preventing and controlling bradysia odoriphaga, and application of bacillus thuringiensis
  • Bacillus thuringiensis for preventing and controlling bradysia odoriphaga, and application of bacillus thuringiensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: the preparation of JQ23 bacterial strain microbial insecticide

[0046] Proceed as follows:

[0047] (1) Strain activation: the preserved Bacillus thuringiensis (Bacillus thuringiensis) strain JQ23 ((had been preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on June 24, 2014, preservation address: Beichen, Chaoyang District, Beijing Institute of Microbiology, Chinese Academy of Sciences, No. 1 Yard, West Road No. 3, the preservation number is CGMCC No.9375)) in LB plate medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 10g , agar powder 15g, water 1L, pH7.2) activated at 29°C for 13h, picked a single colony in LB slant medium ((its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 10 g, 15 g of agar powder, 1 L of water, pH7.2) at 30 ° C for 3 days to obtain activated strains.

[0048] (2) Preparation of seed liquid: use a st...

Embodiment 2

[0051] Embodiment 2: Indoor bioassay test of JQ23 bacterial strain of the present invention to prevent and treat tardy-eyed fungus larvae

[0052] (1) Experimental design:

[0053] The JQ23 bacterium liquid that embodiment 1 prepares is diluted with water respectively to 5 * 10 8 , 1×10 8 , 1×10 7 , 1×10 6 , 1×10 5 cfu / ml five concentration gradients.

[0054] Control: sterile water

[0055] (2) Test method:

[0056] (1) Pour sterilized 1.5% water agar into a disposable plastic petri dish with a thickness of 3mm, and spread two layers of filter paper;

[0057] (2) Cut leeks into leek segments with a size of 10 mm, soak them in the JQ23 bacterial solution prepared in Example (1) for 20 minutes, take out the leek segments soaked in the JQ23 bacterial solution and put them in a plate.

[0058] (3) Pick the same size, healthy and lively larvae of the tardigrade larvae and inoculate them on the leek segment containing bacteria in step (2), and use sterile water as a control...

Embodiment 3

[0063] Embodiment 3, field comparative test of Bacillus thuringiensis JQ23 of the present invention to prevent and control tardy-eyed fungus

[0064] (1) Test drug

[0065] (1), process I: the JQ23 bacterium liquid that embodiment 1 prepares, concentration is 1 * 10 7 cfu / ml.

[0066] (2), treatment II: chlorpyrifos emulsifiable concentrate (480g / L) (Qingdao Jinzheng Pesticide Co., Ltd.), diluted 1000 times with water.

[0067] (3), treatment III: clear water (blank control)

[0068] (2) Test time and place: In April 2014, it was carried out in the greenhouse of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.

[0069] (3) Test method: Divide the leek field planted in the greenhouse into 20m 2 / plot, each treatment was repeated 3 times, and placed in random groups. In the early stage of leek maggot occurrence, according to each treatment in (1), the root irrigation method is used for processing, and the water consumption of each plot...

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Abstract

The invention belongs to the field of biological prevention and control, and discloses a bacillus thuringiensis strain JQ23 for preventing and controlling bradysia odoriphaga. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC No. 9375 on June 24, 2014. The invention further discloses a microbial insecticide containing JQ23, and a preparation method of the microbial insecticide. The bacillus thuringiensis JQ23 has very high toxicity to a bradysia odoriphaga larva, and provides an effective method for prevention and control of the bradysia odoriphaga; secondly, the bacillus thuringiensis belongs to a natural product, is nontoxic to people and livestock, and is pollution-free to an environment; and in addition, the microbial insecticide is simple in preparation method and convenient to use.

Description

technical field [0001] The invention belongs to the field of biological control, and in particular relates to a bacillus thuringiensis used for preventing and treating tardyophthora tardigrades and the application of the bacillus thuringiensis in the prevention and control of vegetable pests. Background technique [0002] Bradysia odoriphaga, also known as chive maggots and yellow-footed mushroom mosquitoes, belongs to the order Diptera and belongs to the family Oculiidae. It mainly harms leeks, green onions, onions, shallots, garlic and other Liliaceae vegetables, and occasionally lettuce, Green cabbage, celery, etc. are one of the main pests of onion and garlic vegetables, and they are distributed all over the country. The larvae of the tardy-eyed fungus live on the soil surface, and they gather in the underground bulbs and tender stems of the chives to eat and eat. The newly hatched larvae first damage the base of the leek leaf sheath and the upper end of the bulb; in sp...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P7/04C12R1/07
Inventor 杜立新宋健曹伟平冯书亮王金耀
Owner WUHAN KERNEL BIO-TECH CO LTD
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