Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for directly measuring content of cordyceps sinensis polysaccharide peptide in cordyceps sinensis product

A technology of Cordyceps sinensis polysaccharide and Cordyceps sinensis, which is applied in the field of food inspection and analysis, and achieves the effect of simple and convenient pretreatment and easy popularization

Inactive Publication Date: 2014-10-08
黄宏南
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no record of a rapid, accurate, and easy-to-popular detection method for the Cordyceps sinensis polysaccharide peptide in Cordyceps sinensis products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for directly measuring content of cordyceps sinensis polysaccharide peptide in cordyceps sinensis product
  • Method for directly measuring content of cordyceps sinensis polysaccharide peptide in cordyceps sinensis product
  • Method for directly measuring content of cordyceps sinensis polysaccharide peptide in cordyceps sinensis product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method provided by the invention is used to measure the polysaccharide peptide of Cordyceps sinensis in Qinghai Jiadui Cordyceps sinensis pure powder sample.

[0027] 1. The instruments and medicines used in the determination include: Waterse2695 high performance liquid chromatography system, Waterse2996 diode array detector, Empower chromatography workstation and data processing system; Cordyceps polysaccharide peptide standard (>98%, National Juncao Engineering Technology Research center);

[0028] 2. The determination steps are as follows:

[0029] (1) Sample processing

[0030] Weigh 0.25g of pure powder sample of Qinghai Jiadui Cordyceps sinensis, add 10ml of water and extract in 60°C water bath for 30min, after cooling, add water to 50ml to make it an aqueous solution of Cordyceps sinensis product with a concentration of 5.00mg / ml, filter This solution, take 2ml of filtrate, add 6ml of absolute ethanol and then centrifuge 15min by centrifuge, the rotating sp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for simply, quickly, directly and accurately measuring the connect of cordyceps sinensis polysaccharide peptide in a cordyceps sinensis product. The method comprises the following steps: performing simple pretreatment on a sample, and qualitatively and quantitatively analyzing through a high performance liquid chromatography photo-diode array (HPLC-PDA) detector. By utilizing the advantages of accurate quantification of high performance liquid chromatography, accurate spectral analysis qualification of the PDA detector and the like, the method can be used for simply, quickly, accurately and directly measuring the connect of cordyceps sinensis polysaccharide peptide in the ordyceps sinensis product, and a simple, reliable and easily-popularized method is supplied to authentication analysis, quality evaluation and quality control of health efficacy factors in the cordyceps sinensis product.

Description

technical field [0001] The invention belongs to the field of food inspection and analysis, and specifically relates to a method for accurately and rapidly measuring the content of cordyceps sinensis polysaccharide peptides in cordyceps sinensis products by using the HPLC-PDA method. Background technique [0002] Cordyceps sinensis (scientific name: Ophiocordyceps sinensis), also known as Chinese caterpillar fungus, is a traditional precious Chinese medicinal material in Chinese history. It is mainly produced in the alpine regions and snow-capped grasslands of Qinghai, Tibet, Sichuan, Yunnan, Gansu and Guizhou provinces and autonomous regions in mainland China. Modern medicine has proved that Cordyceps sinensis has the effects of calming down, relieving cough and reducing phlegm, inhibiting the growth of tumor cells, increasing the body's unique immunity to fitness and disease prevention, and the polysaccharide peptide of Cordyceps sinensis is one of the main functional ingred...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02
Inventor 黄宏南吴志仁王娟娟
Owner 黄宏南
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com