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SNPs primers for detecting type 2 diabetes risk and application thereof

A type 2 diabetes and risk technology, applied in the field of SNPs primers, can solve the problem of low risk accuracy and achieve the effect of increasing body movement, high efficiency, and improving accuracy

Inactive Publication Date: 2014-10-08
NANJING MEDICAL UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since type 2 diabetes is a chronic metabolic disorder associated with the dysfunction of multiple proteins, the accuracy of detecting the risk of type 2 diabetes with a single SNP is low

Method used

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  • SNPs primers for detecting type 2 diabetes risk and application thereof
  • SNPs primers for detecting type 2 diabetes risk and application thereof
  • SNPs primers for detecting type 2 diabetes risk and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Sample collection and arrangement:

[0042] The inventor collected blood samples from 222 patients with type 2 diabetes and 140 normal controls from Ningbo Lihuili Hospital between May 2011 and April 2012 for research.

[0043] DNA was extracted using whole blood genomic DNA extraction kit (BioTeke, China), and the specific steps were as follows:

[0044] 1) Add 900 μL red blood cell lysate to a 1.5 mL microcentrifuge tube.

[0045] 2) Mix the anticoagulated blood thoroughly, and add 300 μL to the erythrocyte lysate in step 1.

[0046] 3) Let stand at room temperature for 10 minutes.

[0047] 4) Centrifuge at 12000 rpm for 20 seconds. Carefully remove the red supernatant and vortex for 15 seconds.

[0048] 5) Add 300 μL of nuclear lysate, and flick the centrifuge tube several times quickly and vigorously.

[0049] 6) Add 30 μg / mL RNase A, mix thoroughly, incubate at 37°C for 15 minutes, and then let stand to room temperature.

[0050] 7) Add 100 μL of protein prec...

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Abstract

The invention discloses SNPs primers for detecting type 2 diabetes risk and application thereof. The SNPs primers comprise three kinds of SNPs primers, and the three kinds of SNPs primers comprise CDK5 regulatory subunit associated protein 1-like SNP rs7756992, voltage-gated potassium channel KQT-like subfamily member 1SNPrs2237892, insulin-like growth factor 2mRNA binding protein SNPrs4402960. By using the primer combination disclosed by the invention to detect whether three kinds of single nucleotide polymorphism exist in DNA, high-risk crowds can be determined, so that the crowds are assisted to adjust life habit and the risk of type 2 diabetes is reduced, and also regular monitoring is performed, so that the symptoms of type 2 diabetes can be discovered early and treatment is facilitated.

Description

technical field [0001] The invention belongs to the field of biology, and relates to a SNPs primer for detecting the risk of type two diabetes and its application. Background technique [0002] Type 2 diabetes is the most common type of diabetes, and its main symptoms are hyperglycemia, varying degrees of insulin resistance, and impaired insulin secretion. Type 2 diabetes can bring various inconveniences to patients, such as thirst, weakness, and vision loss. Excessive blood sugar concentration in the blood will cause damage to blood vessels and organs, cause various complications, and cause great pain to patients. There are environmental factors, lifestyle factors, age factors, etc. in its pathogenesis, and genes are also one of the important reasons. [0003] Diabetes is currently diagnosed based on blood sugar levels. But by the time the blood sugar level rises abnormally to the diagnostic standard, it is often too late. As a chronic metabolic disorder, type 2 diabete...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 刘宁生
Owner NANJING MEDICAL UNIV
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