Human tissue kallikrein activity detection kit and its application
A kallikrein and activity detection technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of unfavorable TK activity detection and complex protein components, and achieve the effects of high sensitivity, good repeatability and strong specificity
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[0041] In order to better explain the present invention, the main content of the present invention is further clarified below in conjunction with specific examples, but the content of the present invention is not limited to the following examples.
[0042] Preparation of Human Tissue Kallikrein Activity Detection Kit
[0043] 1. Reagents
[0044] 1) Coating buffer (pH=9.6 / 0.05 carbonate buffer):
[0045]
[0046] 2) PBST diluted washing solution (pH=7.4 / 0.15M)
[0047]
[0048] 3) BAEE working fluid
[0049]Dilute 1.7191 g of BAEE powder to 100 ml with Tris-HCl buffer solution at pH=8.0, which is 0.05 MBAEE.
[0050] 4) Other reagents
[0051] Dilute hydrochloric acid is made from concentrated hydrochloric acid (concentration about 37%) and water in a volume ratio of 1:2, and its mass fraction is 13.73%.
[0052] The equivalent concentration of sodium hydroxide solution is 3.5N, the molar concentration of ferric chloride solution is 0.11M and the molar concentration...
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