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A kind of preparation method of peptide chitosan/hydroxyapatite/pearl powder scaffold

A technology of hydroxyapatite and chitosan, which is applied in the field of medical materials, can solve the problems of low osteogenesis efficiency and achieve good osteoconductivity

Inactive Publication Date: 2016-08-17
TSINGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chitosan has osteoconductivity, it only acts as a physical scaffold and has low osteogenesis efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0021] The preparation method of the polypeptide chitosan / hydroxyapatite / pearl powder support that the present invention proposes comprises the following steps:

[0022] (1) Chitosan is dissolved in the acetic acid aqueous solution that volume concentration is 1%, and the mass volume ratio concentration of chitosan and acetic acid aqueous solution is 1~2mg / mL, obtains the acetic acid solution of chitosan;

[0023] (2) Dissolve 2-iminosulfane hydrochloride in the chitosan acetic acid solution obtained in the above step (1), and form a mixed solution after completely dissolving, adjust the pH value of the mixed solution to 4~6 with NaOH solution, and continuously Stir for 24 to 30 hours;

[0024] (3) Place the mixed solution obtained in step (2) in a dialysis bag with a molecular weight cut-off of 8,000 to 14,000, perform dialysis for 4 to 6 days, and record the product in the dialysis bag as the dialysis product;

[0025] (4) pre-freezing the dialysis product obtained in step ...

Embodiment 1

[0036] (1) Chitosan (can be purchased from reagent company) is dissolved in the acetic acid aqueous solution of 1% by volume concentration, and the mass volume ratio concentration of chitosan and acetic acid aqueous solution is 2mg / mL, obtains the acetic acid solution of chitosan ;

[0037] (2) 2-iminosulfane hydrochloride (can be purchased from the reagent company) is dissolved in the chitosan acetic acid solution that above-mentioned step (1) obtains, becomes mixed solution after dissolving completely, regulates the mixed solution with NaOH solution pH value is 6, continuous stirring for 240 hours;

[0038] (3) Place the mixed solution obtained in step (2) in a dialysis bag with a molecular weight cut-off of 8000 to 14000, perform dialysis for 5 days, and record the product in the dialysis bag as the dialysis product;

[0039] (4) The dialysis product obtained in step (3) was pre-frozen at -20°C for 24 hours, and freeze-dried in a vacuum freeze dryer for 72 hours to obtain ...

Embodiment 2

[0049] (1) Chitosan is dissolved in the acetic acid aqueous solution that volume concentration is 1%, and the mass volume ratio concentration of chitosan and acetic acid aqueous solution is 1mg / mL, obtains the acetic acid solution of chitosan;

[0050] (2) 2-iminosulfane hydrochloride is dissolved in the chitosan acetic acid solution that above-mentioned step (1) obtains, becomes mixed solution after dissolving completely, adjusts the pH value of mixed solution with NaOH solution to be 4, continuously stirs 30 Hour;

[0051] (3) Place the mixed solution obtained in step (2) in a dialysis bag with a molecular weight cut-off of 8000 to 14000, perform dialysis for 4 days, and record the product in the dialysis bag as the dialysis product;

[0052] (4) The dialysis product obtained in step (3) was pre-frozen at -20° C. for 30 hours, and freeze-dried in a vacuum freeze dryer for 80 hours to obtain thiolated chitosan;

[0053] (5) the thiolated chitosan that step (4) obtains is dis...

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Abstract

The invention relates to a preparation method of a scaffold connected with polypeptide chitosan / hydroxyapatite / pearl powder, which belongs to the technical field of medical materials. First, chitosan was dissolved in acetic acid aqueous solution, and then 2-iminosulfane hydrochloride was dissolved in it, the pH value was adjusted, dialyzed, and freeze-dried to obtain thiolated chitosan; bone morphogenetic protein-2 polypeptide Dissolved in dimethyl sulfoxide, added to the aqueous solution of thiolated chitosan to obtain the peptide chitosan; finally dissolved the peptide chitosan in hydrochloric acid, added pearl powder and hydroxyapatite powder, filled Put it in a cylindrical mold and freeze-dry to obtain a polypeptide-chitosan / hydroxyapatite / pearl powder scaffold. The advantage of the method of the present invention is that the BMP-2 polypeptide is grafted onto the chitosan through a chemical method. In addition to good osteoconductivity, degradability, and cell adhesion, the scaffold material also has osteogenic induction due to the presence of polypeptide and pearl powder organic matrix, and can be used for bone defect repair.

Description

technical field [0001] The invention relates to a preparation method of chitosan / hydroxyapatite / pearl powder scaffold grafted with polypeptide, in particular to a preparation method of chitosan / hydroxyapatite / pearl powder scaffold grafted with BMP-2 polypeptide, It belongs to the technical field of medical materials. Background technique [0002] The repair of large-scale bone defects is a difficult problem in clinical treatment. Tissue engineering can repair bone defects through scaffold materials combined with cells and growth factors, which has become a new way of bone repair. How to construct a bone tissue that can support cell growth, control cell behavior, and Inducible properties of scaffold materials are a key issue in bone tissue engineering. In the field of bone defect repair, there are many ways to construct scaffold materials, of which there are two main ones: one is chemical modification, which uses covalent bonding to graft proteins or active peptides onto sca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/28C08L5/08C08K3/26C08K3/32C08B37/08A61L27/46A61L27/56
Inventor 冯庆玲刘旭杰于博陈彦刘睿
Owner TSINGHUA UNIV
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