Preparation method for calibration matter for calibrating apolipoprotein A1 and apolipoprotein B

Abstract

The invention discloses a preparation method for a calibration matter for calibrating apolipoprotein A1 and apolipoprotein B. The essentials of the technical scheme are as follows: the preparation method comprises the following steps: (1) adding a multi-anionic compound and divalent metal ions into blood serum to carry out pre-treatment; (2) extracting low density lipoprotein (LDL); (3) extracting high density lipoprotein (HDL); (4) mixing protein and base blood serum to prepare the calibration matter; (5) defining the value of the calibration matter. The preparation method has the advantages that the extraction of LDL and HDL components in the blood serum by a multi-anionic compound and divalent metal ion sub-step precipitation method is simplified; the content of the ApoB and the content of the ApoA1 are detected; the calibration matter can be mixed into the blood serum of healthy people according to the needed amount so that the ApoA1 and the ApoB reach the predicated content; the method is simple; a stabilizer and a turbidity-preventing agent are added into the mixed blood serum so that the prepared freeze-dried calibration matter is clarified after being re-dissolved; the stability is good and a base material effect does not exist; the calibration matter is diluted into five different concentrations and can be used for non-linear calibration of determining the ApoA1 and the ApoB on an automatic analyzer.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for preparing apolipoprotein A1 and apolipoprotein B calibrator. Background technique [0002] Clinically, the determination of serum apolipoprotein A1 (ApoA1) and apolipoprotein B (ApoB) is often used as the risk estimation of cardiovascular and cerebrovascular diseases, the monitoring of the efficacy of drug treatment, and the diagnosis of certain abnormal lipoproteinemia. The commonly used serum ApoA1 and ApoB assay kits in China all adopt Immunoturbidimetric assay (ITA), because it is fast, accurate, precise and suitable for various types of automatic biochemical analyzers, especially suitable for clinical laboratories. Determination of large batches of specimens. However, multiple points (generally 5 different concentrations) are required for nonlinear calibration during the determination, so a calibrator with a high-end concentration of the ApoA1 and ApoB measurement ra...

AI Technical Summary

Problems solved by technology

[0004] When preparing Apoa1 and Apob calibrants by traditional methods, human serum is directly subjected to ultracentrifugation, which not only requires many times of centrifugation and takes a long time, but also cannot separate other proteins in the serum, resulting in a waste of resources. After dissolution, the solution is easily turbid, the stability is average, and there will be a certain matrix effect