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Uricase multivesicular liposome and preparation method thereof

A multivesicular liposome and uricase technology, which can be used in liposome delivery, pharmaceutical formulations, peptide/protein components, etc., can solve the problem of low uricase activity, easy induction of immune responses, and immunogenicity and antigenic reactions And other issues

Active Publication Date: 2014-09-03
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Uricase multivesicular liposomes can overcome the disadvantages of low activity of uricase in vivo, short circulation half-life, and easy induction of immune responses in the body, resulting in immunogenicity and antigenic reactions.

Method used

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  • Uricase multivesicular liposome and preparation method thereof
  • Uricase multivesicular liposome and preparation method thereof
  • Uricase multivesicular liposome and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The composition ratio by weight of each component contained in the formula is: 1 part of lecithin, 1 part of cholesterol, 1 part of triolein, 2 parts of glucose (I), 3.4 parts of poloxamer F-68, poloxamer F-12715 parts, borate buffer pH8 (I) 80 parts, L-lysine 1 part, glucose (II) 10 parts, borate buffer pH8 (II) 200 parts.

[0018] The preparation method comprises the following steps: (1) preparation of colostrum: get uricase, poloxamer F-127, poloxamer F-68, glucose (I) of prescription quantity and be dissolved in the borate buffer solution of prescription quantity In (I), the phospholipids, cholesterol, and triolein of the prescribed amount are dissolved in ether as the oil phase, and the borate buffer (I) is mixed with the oil phase, and W / O is prepared under vortex conditions. Type colostrum; (2) double milk preparation: get the borate buffer solution (II) that the L-lysine of prescription quantity and glucose are dissolved in prescription quantity, add colostrum t...

Embodiment 2

[0020] The composition ratio by weight of each component contained in the formula is: 1.3 parts of lecithin, 1.4 parts of cholesterol, 1.3 parts of triolein, 4 parts of glucose (I), 4.8 parts of poloxamer F-68, poloxamer F-12721 parts, borate buffer pH8.2 (I) 95 parts, L-lysine 2.2 parts, glucose (II) 12 parts, Bicine buffer pH8.7 (II) 360 parts.

[0021] The preparation method is basically the same as in Example 1.

Embodiment 3

[0023] The composition ratio by weight of each component contained in the formula is: 1.6 parts of lecithin, 1.6 parts of cholesterol, 1.5 parts of triolein, 5.5 parts of glucose (I), 6.5 parts of poloxamer F-68, poloxamer F-12728 parts, borate buffer pH8.9 (I) 115 parts, L-lysine 1.2 parts, glucose (II) 10.5 parts, Tris buffer pH8.3 (II) 230 parts.

[0024] The preparation method is basically the same as in Example 1.

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Abstract

The invention belongs to the field of pharmaceutical preparations, and relates to a formula and a preparation method of uricase multivesicular liposome. The prepared uricase multivesicular liposome can improve the activity of uricase and reduce the immunogenicity thereof; moreover, the uricase can be slowly released to prolong the action time and efficacy time of a drug.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and relates to a uricase multivesicular liposome and a preparation method thereof. Background technique [0002] Uric acid is the end product of the catabolism of purine bases in the human body and is excreted by the kidneys. When the production of uric acid in the body exceeds the renal excretion, the serum uric acid will increase significantly, resulting in hyperuricemia. Hyperuricemia is closely related to the initiation or exacerbation of gout, cardiovascular disease, tumor lysis syndrome and kidney disease. Therefore, there is a need to develop safe and effective drugs to treat hyperuricemia. The excellent water solubility of allantoin and the efficient excretion ability of the kidney make uricase an ideal drug for the treatment of hyperuricemia. Clinical studies have shown that uricase can quickly and efficiently reduce blood uric acid with high specificity. In the treatment of...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K38/44A61P19/06
Inventor 张景勍熊华蓉周岐新周云莉何丹胡雪原
Owner CHONGQING MEDICAL UNIVERSITY
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