Porcine circovirus type 2 (PCV2) IgM antibody colloidal gold immunochromatographic assay test paper, and preparation method and application thereof
An immunochromatographic detection, porcine circovirus technology, applied in biological testing, measuring devices, analytical materials, etc., can solve the problems of inability to distinguish specific PCV2IgM antibodies, requiring specialized instruments and personnel, and rarely detecting IgM antibodies, etc. Achieve intuitive and easy-to-recognize results, easy operation, and fast detection.
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Embodiment 1
[0041] The schematic diagram of the structure of the porcine circovirus type 2 IgM antibody colloidal gold immunochromatographic detection test paper card is as follows figure 1 As shown, the horizontal surface of the test strip in the test paper card is sequentially divided into water absorption area 1, solidified antibody area 2, gold standard probe area 3, and sample absorption area 4 from left to right. After the test strip is loaded into the card case 6, it is the porcine circovirus type 2 IgM antibody colloidal gold immunochromatography test card; water absorption area 1, solidified antibody area 2, gold standard probe area 3, sample absorption area 4, bottom plate The materials of 5 are water-absorbing filter paper, nitrocellulose membrane, glass fiber membrane, polyester fiber membrane and polyethylene plate, and the cartridge 6 is a plastic cartridge with a viewing window and a sample hole. The detection line T 7 of the solidified antibody area 2 is coated with mouse ...
Embodiment 2
[0059] Except detection line T 7 coated with mouse anti-pig IgM monoclonal antibody amount of 3 μg / cm; control line C 8 coated with goat anti-mouse IgG amount of 7 μg / cm; gold standard probe region 3 coated with colloidal gold The labeling amount of the labeled anti-porcine circovirus type 2 Cap protein monoclonal antibody is 12 μg / mL, and the coating volume is 30 μL / cm; the method for colloidal gold-labeled anti-porcine circovirus type 2 Cap protein monoclonal antibody: take the radius 20mL of 40nm colloidal gold with a pH value of 9.2 and a concentration of 0.01%, added 240 μg of monoclonal antibody against porcine circovirus type 2 Cap protein, combined under stirring conditions, added BSA to a final concentration of 7%, centrifuged to remove untreated Combined anti-porcine circovirus type 2 Cap protein monoclonal antibody and unstabilized colloidal gold particles and agglutinates, and the deep red precipitate at the bottom of the centrifuge tube was dissolved with 2mL of 0....
Embodiment 3
[0061] The method of using the above-mentioned porcine circovirus type 2 IgM antibody colloidal gold immunochromatography test paper card:
[0062] Dilute 3-4 drops of the serum sample to be tested with 1mL 0.01M PBS with a pH value of 7.4, then drop 4-5 drops into the sample hole, and judge the result within 10-15 minutes. The red color of the control line C indicates that the test card is valid. Both the test line T and the test line T appear red, indicating that the sample IgM antibody is positive, and only the control line C appears red, indicating that the sample IgM antibody is negative.
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