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Imnetic algae strain Chlorella sorokiniana HN01 and application thereof

A CCTCCM2013608, microalgae technology, applied in the direction of single-cell algae, microorganisms, biochemical equipment and methods, etc., can solve problems such as difficult large-scale high-density cultivation, high energy consumption costs in the treatment process, water source pollution, etc., to ensure high density High-quality fermentation and the effect of enhancing international competitiveness

Inactive Publication Date: 2014-07-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, the production process of domestic chlorella is relatively open, and it is difficult to ensure the consistency of batches and the pollution of foreign organisms and water sources. In addition, it is difficult to achieve large-scale high-density cultivation of traditional chlorella cultivation as autotrophic cultivation. High energy consumption and high cost

Method used

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  • Imnetic algae strain Chlorella sorokiniana HN01 and application thereof
  • Imnetic algae strain Chlorella sorokiniana HN01 and application thereof
  • Imnetic algae strain Chlorella sorokiniana HN01 and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0060] Prepare 500mL of BG-11 medium, and insert it into the microalgae seed liquid that has been expanded and cultivated according to the inoculation amount of 15v / v%. The initial pH is 4.0, the temperature is 28°C, the light-dark ratio is 12:12 (day: night), the light intensity is 15000lux, the amount of glucose added is 2g / L, and the sterile air of 0.2vvm is passed through, and cultivated for 14 days.

[0061] The algal cells were collected by centrifugation, dried in a vacuum freeze dryer, and the algal powder was weighed and the dry weight was calculated. The biomass concentration of algae powder is 700mg / L, the biomass yield is 50mg / L / d, and the oil content determined by the chloroform-methanol method is 22%. The protein content is more than 40%, and the linoleic acid and linolenic acid account for more than 40% of the total fatty acid composition. The HPLC spectrum of the fatty acid methyl ester composition is as follows Figure 4 shown.

[0062] Example 2

Embodiment 2

[0064] Prepare 500mL of BG-11 medium, and insert it into the expanded cell culture medium according to the inoculation amount of 5v / v%. The initial pH is 8.0, the temperature is 28°C, the light-dark ratio is 12:12 (day: night), the light intensity is 7500lux, the amount of glucose added is 15g / L, and the sterile air of 0.33vvm (CO2 content is 0.03% (v / v) ), cultivated for 9 days.

[0065] The algal cells were collected by centrifugation, dried in a vacuum freeze dryer, and the algal powder was weighed and the dry weight was calculated. The biomass concentration of algal powder was 6.2g / L, the biomass yield was 0.67g / L / d, and the oil content was 43% as determined by the chloroform-methanol method. The protein content is more than 38%, and linoleic acid and linolenic acid account for more than 20% of the total fatty acid composition. The scanning electron microscope photos, optical microscope photos, and fat droplet staining photos of microalgae cells are shown in Figure 1-3 ...

Embodiment 3

[0068] Prepare 500mL of BG-11 medium, and insert the expanded microalgae cells. The initial pH is 11.0, the temperature is 45°C, the light-to-dark ratio is 12:12 (day:night), the light intensity is 2500lux, the amount of glucose added is 15g / L, and the sterile air of 0.33vvm (CO2 content is 0.03% (v / v) ), cultured for 5 days.

[0069] The algal cells were collected by centrifugation, dried in a vacuum freeze dryer, and the algal powder was weighed and the dry weight was calculated. The biomass concentration of algae powder was 4.4g / L, the biomass yield was 0.49g / L / d, and the oil content was 31% as determined by the chloroform-methanol method. The protein content is more than 40%, and the linoleic acid and linolenic acid account for more than 20% of the total fatty acid composition.

[0070] Example 4

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Abstract

The invention relates to the technical field of biomass energy, and particularly relates to an imnetic chlorella strain Chlorella sorokiniana HN01 and application thereof to high density fermentation for production of chlorella nutritional health products. The imnetic chlorella strain Chlorella sorokiniana HN01 provided by the invention has a preservation number of CCTCC M2013608, can realize high-density high-quality fermentation in a photoheterotrophy-chemoheterotrophy light switching cultivation mode. Chlorella fermented algae powder has biomass of 6. 2g / L, protein content of 39%, and content of essential fatty acid including linoleic acid and linolenic accounting for more than 20% of total fatty acid content. The invention provides a good algae strain and key fermentation technology for wide application of chlorella in the industries of food, nutritional health products, cosmetics medicine and chemical engineering, and can ensure high-density high-quality fermentation of chlorella and enhance the international competitiveness of China's chlorella industry.

Description

technical field [0001] The invention relates to the technical field of biomass energy, in particular to a freshwater algal strain Chlorella sorokiniana HN01 and its application in the production of chlorella nutrition and health products by a high-density fermentation method. Background technique [0002] Algae are the oldest of all plants. According to the color of the pigment, it can be divided into three categories: green algae, brown algae and red algae; according to different ecological habits, algae can be divided into planktonic algae and benthic algae; algae can be divided into freshwater algae, seaweed, and salt water according to the growth area. Algae, flowing algae, aerial algae, soil algae, hot spring algae, ice and snow algae, and epiphytic algae; Chinese scholars generally divide algae into 11 categories: cyanobacteria, red algae, cryptophytes, dinoflagellates, golden algae, and yellow algae , diatoms, brown algae, euglena, green algae, charophytes. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/62C12P7/64C12R1/89
Inventor 缪晓玲巩三强
Owner SHANGHAI JIAO TONG UNIV
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