Forest frog oil polypeptide microencapsulation and preparation method thereof

A wood frog oil and microcapsule technology, which is applied in the field of microcapsule technology and food processing, can solve the problems of high viscosity, high water content, and long dispersion time of the wood frog oil polypeptide solution, and achieve shortened dissolution time and high dry matter content , the effect of improving solubility

Inactive Publication Date: 2014-07-23
JILIN UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] However, the enzymatically processed Rana oil polypeptide solution still has the problems of high viscosity, difficulty in concentration, and difficulty in processing, and because the raw material of frog oil itself is very precious, the purification of Rana oil polypeptide will cause a certain degree of waste of raw materials; However, the Rana oil polypeptide itself has a bitter and fishy smell, and is easily oxidized and inconvenient to preserve. The Rana oil polypeptide powder obtained by direct milling has a large number of hydrophobic groups, high water content, poor dispersibility, and easy to absorb moisture and agglomerate. The dispersion time in water is long; and there are few related studies on the microencapsulation of Rana oil peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] a. Preparation of wood frog oil enzymatic hydrolysis solution: take dry wood frog oil, add distilled water with a weight ratio of 1:50 to 200, and soak for 12 to 24 hours. Frog oil dry product weight 1-5% protease, heat preservation at 40-50°C for 2-8 hours, after deactivation, homogenize at 60-65°C, 10-20MPa, and obtain the Rana oil polypeptide core material solution;

[0026] b. Under stirring conditions, slowly add a small amount of deionized water dissolved (by weight of the core material solution) 1% to -10% concentrated whey protein powder, 1% to 10% Maltodextrin, 0.5% ~ 5% β-cyclodextrin wall material turbid solution, stir until completely dissolved;

[0027] c. Based on the weight of the core material solution, use a small amount of deionized water to dissolve 0.01% to 0.1% of monoglyceride, heat it in a water bath to 60-80°C, vibrate vigorously to form an emulsion, and add it to the mixed solution;

[0028] d. 5000r / min~10000r / min high-speed shear treatment fo...

Embodiment 1

[0031] Take the dry wood frog oil and add distilled water with a weight ratio of 1:100 to soak for 12 hours. After the tissue is mashed, add an appropriate amount of ginger juice to remove the fishy smell. Add protease with a weight equal to 3% of the weight of the dry wood frog oil. Incubate for 8 hours, homogenize at 60°C and 15MPa after deactivating the enzyme, and cool to 65°C; under stirring conditions, slowly add 5.5% concentrated whey protein dissolved in a small amount of deionized water according to the weight of the enzymatic hydrolyzate of Rana oil Powder, 3.5% maltodextrin, 0.9% β-cyclodextrin turbid liquid; according to the weight of Rana oil enzymatic hydrolyzate, dissolve 0.03% monoglyceride in deionized water at room temperature, and heat it in a water bath to 75°C Shake to become milky, add to the mixed solution; after stirring fully, 7000r / min high-speed shear treatment for 10min, homogenize at 5MPa, 65°C; feed rate 10L / h, inlet air temperature 140°C, outlet ...

Embodiment 2

[0033]Take the dry wood frog oil and add distilled water with a weight ratio of 1:120 to soak for 14 hours. After the tissue is mashed, add an appropriate amount of ginger juice to remove the fishy smell. Incubate for 8 hours, homogenize at 60°C and 15MPa after deactivating the enzyme, and cool to 68°C; under stirring conditions, slowly add 8.1% concentrated whey protein dissolved in a small amount of deionized water according to the weight of the enzymatic hydrolyzate of Rana oil Powder, 5.2% maltodextrin, 1.3% β-cyclodextrin turbid solution; according to the weight of Rana oil enzymatic hydrolyzate, dissolve 0.04% monoglyceride in deionized water at room temperature, and heat it in a water bath to 80°C Shake to become milky, add to the mixed solution; after stirring fully, 8000r / min high-speed shearing treatment for 8min, homogeneous at 0MPa, 68°C; at a feed rate of 11L / h, an inlet air temperature of 145°C, an outlet The wind temperature is 62°C, and the fixed bed temperatur...

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Abstract

The invention discloses a preparation method of forest frog oil polypeptide microencapsulation. The preparation method comprises the main steps of carrying out controllable enzymatic hydrolysis on forest frog oil to obtain a polypeptide core material solution, slowly adding a wall material under the mixing condition (wherein the wall material is prepared from the following components in percentage by weight: 1-10 percent of concentrated whey powder, 1-10 percent of maltodextrin and 0.5-5 percent of beta-cyclodextrine), adding 0.1-1 percent of monoglyceride, carrying out high-speed shearing and homogenizing treatment to obtain an emulsified feed liquid, and carrying out low-temperature spray drying treatment to obtain forest frog oil polypeptide microcapsule powder. With the adoption of the forest frog oil polypeptide microencapsulation, the content of a forest frog oil polypeptide core material is above 10 percent, the embedding rate is above 60 percent, the powder collection rate is above 53 percent, and the sensory property of the forest frog oil polypeptide microencapsulation powder is good without fishy smell; compared with core material spray drying powder without microencapsulation, the forest frog oil polypeptide microencapsulation powder has the advantages that the moisture content is lowered by above 30 percent, the solubility is increased by 3.5 percent, the hygroscopicity is lowered by 22 percent, and the dispersion time is shortened by above 30 percent. The preparation method has the characteristics that by virtue of the microencapsulation method, the fishy smell of a forest frog oil polypeptide product is removed effectively, the raw material waste is avoided, the operation is simplified and industrial production can be promoted.

Description

technical field [0001] The invention relates to a preparation method of Rana oil polypeptide microencapsulation, and relates to the fields of microcapsule technology and food processing technology. Background technique [0002] Rana oil is the dried fallopian tube of the female Rana, and it is a precious nourishing health product, which has the functions of nourishing the kidney and essence, moistening the lungs and nourishing yin. It is rich in protein, 18 kinds of amino acids, a variety of trace elements and vitamins, especially three sex hormones, namely estradiol, testosterone and progesterone. Rana oil enzymatic hydrolysis solution is to hydrolyze Rana oil through controllable enzyme engineering technology, decompose protein into polypeptides, improve the fluidity and solubility of Rana oil, reduce viscosity, and improve the processing of its industrial production Performance; due to the increase in the content of peptides and amino acids after enzymolysis, it is easie...

Claims

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Application Information

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IPC IPC(8): A23L1/29A23L33/00
CPCA23L33/00A23P10/30A23V2002/00A23V2250/54252A23V2300/26A23V2300/41A23V2300/10
Inventor 张铁华徐向秀叶海青姜楠
Owner JILIN UNIV
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