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A method for the determination of nitrofuran antibiotics in cosmetics

A technology of nitrofuran and determination method, which is applied to measurement devices, instruments, scientific instruments and other directions, can solve the problems of auditory hallucinations, hallucinations, irritability, protracted and difficult to heal, etc., achieves accurate determination method, high specificity and sensitivity, avoids The effect of false positive results

Active Publication Date: 2016-03-09
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Nitrofuran drugs are quickly absorbed after entering the human body, and their main toxic and side effects are as follows: (1) They have strong toxic effects on the human body, and long-term or high-dose exposure to nitrofuran drugs can produce toxic effects on the human body , of which nitrofurazone is the most toxic, and furazolidone is relatively less toxic; (2) carcinogenic, teratogenic and mutagenic side effects: furaltadone is a strong carcinogenic drug, and furazolidone is a moderate carcinogenic drug; (3) peripheral neuritis, manifested as hands and feet Numbness, which can cause muscle atrophy over time, often protracted and difficult to heal; (4) allergic reactions (including asthma, chest tightness, rash, drug fever, eosinophilia); (5) gastrointestinal reactions and toxic mental symptoms such as hallucinations Hearing, hallucinations, irritability, etc.; (6) Hemolytic anemia, jaundice, pulmonary complications, etc.

Method used

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  • A method for the determination of nitrofuran antibiotics in cosmetics
  • A method for the determination of nitrofuran antibiotics in cosmetics
  • A method for the determination of nitrofuran antibiotics in cosmetics

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Experimental program
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Effect test

Embodiment 1

[0036] 1. Instruments and reagents

[0037] Agilent1200 liquid chromatograph (with diode array detector) (Agilent, USA); ultraviolet-visible spectrophotometer (SHIMADZUUV-3600); electronic balance (AB204-S, METTLERTOLEDO, USA); ultrapure water device (Milli-Q, Millipore Company of the United States); high-speed refrigerated centrifuge (CR21G, Hitachi Company of Japan); MS2 vortex oscillator (IKA Company of Germany); nitrogen blowing instrument (N-EVP112, Organomation Associates Inc of the United States); fume hood (FC-150, Dalian Lai Botech Laboratory Equipment Co., Ltd.); microporous membrane (0.22 μm, organic system, Pall Company, USA); methanol and acetonitrile were chromatographically pure (Fisher Company); ammonium acetate was analytically pure (content ≥ 98.0%, Shantou, Guangdong Province City Xilong Chemical Co., Ltd.)

[0038] See Table 4 for detailed information on the four substances of nitrofurantoin, furazolidone, furaltadone and nitrofurazone, and see the structu...

Embodiment 2

[0079] Preparation of standard solution and investigation of its stability

[0080] During the preparation of the standard solution, according to the operating requirements in the literature, when the 1mg / mL storage solution was prepared, it was found that it was difficult to completely dissolve the nitrofurazone standard with a common single organic solvent (acetonitrile and methanol). In the experiment, acetonitrile and methanol were used. The mixed solution in the ratio between was used as the solvent to dissolve the substance, and it was found that when 20% methanol-acetonitrile was used, the compound could be completely dissolved.

[0081] Since the nitrofuran itself is unstable, it will be partially degraded and lost under the conditions of high temperature, air exposure and direct sunlight. Therefore, this experiment investigated the chromatogram changes of the four compounds under the conditions of direct sunlight for 1h, 2h and 3h. ,Such as Figure 4 As shown, the ch...

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Abstract

The invention discloses a method for measuring nitrofuran antibiotics in cosmetics. The method comprises the following steps: (1) pretreating a sample, namely adding the sample into a mixed solution of acetonitrile and methanol in a volume ratio of 70:30, uniformly mixing, performing ultrasonic extraction, centrifuging, airing supernatant till the supernatant is nearly dry, dissolving residues in a mixed solution of a 15mmol / L ammonium acetate solution and acetonitrile according to a volume ratio of 85:15, and sieving the solution through a micro-porous filter membrane with the size of 0.22 micron, thereby obtaining filtrate for later use; (2) performing high performance liquid chromatography separation on the filtrate, eluting a mobile phase by a gradient elution program, and quantifying according to an external standard method; and (3) confirming a detected positive sample by high performance liquid chromatography-mass spectrometry / mass spectrometry. According to the method, four nitrofuran antibiotics such as macrodantin, furazolidone, furaltadone and nitrofurazone are simultaneously detected. The method is accurate, reliable and high in sensitivity.

Description

technical field [0001] The invention relates to a method for determining antibiotics, in particular to a method for determining nitrofuran antibiotics in cosmetics. Background technique [0002] Nitrofuran drugs are a kind of broad-spectrum antibiotics, which are widely used in clinic because of their strong bactericidal ability, broad antibacterial spectrum, resistance to drug resistance, low price and good curative effect. These antibiotics are mainly used in the treatment of urinary system infection, intestinal bacterial infection, skin wound infection and as a food additive to prevent poultry intestinal infectious diseases. The common nitrofuran antibiotics mainly include four kinds: nitrofurantoin (Furadantin, NFT), furazolidone (Furazolidone, FZD), furaltadone (Furaltadone, FTD) and nitrofuran (Furacilin, NFZ), which are all artificially synthesized with 5 - Antibacterial drug with nitrofuran basic structure. It is understood that although nitrofuran drugs will not c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 马强孟宪双白桦陈云霞李晶瑞马会娟王元成
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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