Method for measuring nitrofuran antibiotics in cosmetics

Abstract

The invention discloses a method for measuring nitrofuran antibiotics in cosmetics. The method comprises the following steps: (1) pretreating a sample, namely adding the sample into a mixed solution of acetonitrile and methanol in a volume ratio of 70:30, uniformly mixing, performing ultrasonic extraction, centrifuging, airing supernatant till the supernatant is nearly dry, dissolving residues in a mixed solution of a 15mmol / L ammonium acetate solution and acetonitrile according to a volume ratio of 85:15, and sieving the solution through a micro-porous filter membrane with the size of 0.22 micron, thereby obtaining filtrate for later use; (2) performing high performance liquid chromatography separation on the filtrate, eluting a mobile phase by a gradient elution program, and quantifying according to an external standard method; and (3) confirming a detected positive sample by high performance liquid chromatography-mass spectrometry / mass spectrometry. According to the method, four nitrofuran antibiotics such as macrodantin, furazolidone, furaltadone and nitrofurazone are simultaneously detected. The method is accurate, reliable and high in sensitivity.

Description

technical field [0001] The invention relates to a method for determining antibiotics, in particular to a method for determining nitrofuran antibiotics in cosmetics. Background technique [0002] Nitrofuran drugs are a kind of broad-spectrum antibiotics, which are widely used in clinic because of their strong bactericidal ability, broad antibacterial spectrum, resistance to drug resistance, low price and good curative effect. These antibiotics are mainly used in the treatment of urinary system infection, intestinal bacterial infection, skin wound infection and as a food additive to prevent poultry intestinal infectious diseases. The common nitrofuran antibiotics mainly include four kinds: nitrofurantoin (Furadantin, NFT), furazolidone (Furazolidone, FZD), furaltadone (Furaltadone, FTD) and nitrofuran (Furacilin, NFZ), which are all artificially synthesized with 5 - Antibacterial drug with nitrofuran basic structure. It is understood that although nitrofuran drugs will not c...

AI Technical Summary

Problems solved by technology

[0003] Nitrofuran drugs are quickly absorbed after entering the human body, and their main toxic and side effects are as follows: (1) They have strong toxic effects on the human body, and long-term or high-dose exposure to nitrofuran drugs can produce toxic effects on the human body , of which nitrofurazone is the most toxic, and furazolidone is relatively less toxic; (2) carcinogenic, teratogenic and mutagenic side effects: furaltadone is a strong carcinogenic drug, and furazolidone is a moderate carcinogenic drug; (3) peripheral neuritis, manifested as hands and feet Numbness, which can cause muscle atrophy over time, often protracted and difficult to heal; (4) allergic reactions (including asthma, chest tightness, rash, drug fever, eosinophilia); (5) gastrointestinal reactions and toxic mental symptoms such as hallucinations Hearing, hallucinations, irritability, etc.; (6) Hemolytic anemia, jaundice, pulmonary complications, etc.

Images