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Tough tissue structure and 3D printing forming device and method thereof

A tissue structure, 3D printing technology, applied in the direction of ligaments, prostheses, muscles, etc., can solve the problem of incomplete recovery of damaged tissues, and achieve the effect of low immune rejection

Inactive Publication Date: 2014-07-16
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing elastic tissue repair methods are affected by material properties, biocompatibility and repair regeneration speed, and cannot fully restore the function of damaged tissue

Method used

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  • Tough tissue structure and 3D printing forming device and method thereof
  • Tough tissue structure and 3D printing forming device and method thereof
  • Tough tissue structure and 3D printing forming device and method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0054] The present invention uses the above-mentioned 3D printing forming equipment to prepare a tough tissue structure. The preparation method includes a direct printing forming method in vivo or an external printing forming method. The method includes the following steps:

[0055] 1) In vivo direct printing and forming:

[0056]a) A three-dimensional model of the tough tissue structure is designed by a computer, or a three-dimensional model of the tough tissue structure is obtained by scanning the lesion with a scanning imaging system, and the computer assigns a printing path; the signal obtained by scanning the lesion is scanned by the scanning imaging system Send to the control system 404 for processing to obtain the instruction signal, and send the instruction signal to the rapid prototyping system 402 and the transmission system 403; b) prepare the prepared polymer hydrogel and polymer fiber with a mass volume concentration of 0.1-20% The raw materials are respectively l...

Embodiment 1

[0060] Example 1: Using 3D printing technology to prepare a ligament tissue structure in vitro and implant it in vivo.

[0061] 1) Preparation of cells and hydrogel: extract human fibroblasts, subculture the fibroblasts for later use; dissolve gelatin powder in phosphate buffer to prepare a hydrogel with a mass volume fraction of 15%; mix the fibroblasts into the above hydrogel to obtain a cell concentration of 1 x 10 6 Each / mL cell-hydrogel material system; the cell-hydrogel material is loaded into a 3D printed screw extrusion nozzle;

[0062] 2) Preparation of fiber material: heat polycaprolactone to melt it, and put the melted polycaprolactone into the 3D printed electrospinning nozzle for standby;

[0063] 3) Model design: design a three-dimensional model of the ligament tissue structure, and distribute the printing paths of the fiber layer and the hydrogel layer by the computer;

[0064] 4) Forming process: the 3D printing equipment is controlled by a computer. First, t...

Embodiment 2

[0066] Example 2: Using 3D printing technology to prepare a ligament tissue structure in vitro and implant it in vivo.

[0067] 1) Preparation of cell suspension: extract human fibroblasts, subculture the fibroblasts for later use, prepare the cell suspension of the cells, and the cell concentration is 1×10 6 cells / mL, the cell suspension is loaded into the spray nozzle of the 3D printing device;

[0068] 2) Preparation of hydrogel: dissolving gelatin powder in phosphate buffer solution to prepare hydrogel with a mass volume fraction of 15%, and loading the hydrogel into the screw extrusion nozzle of the 3D printing device;

[0069] 3) Preparation of fiber material: dissolving polyurethane material in tetraethylene glycol solution to obtain a solution with a mass volume fraction of 10%, and putting the solution into the screw extrusion nozzle of the 3D printing equipment;

[0070] 4) Model design: the patient's ligament injury site is scanned by the scanning imaging system to...

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Abstract

The invention discloses a tough tissue structure and a 3D printing forming device and method of the tough tissue structure, and belongs to the field of composite materials, tissue engineering and medical apparatuses and instruments. The tough tissue structure is a three-dimensional structure, and comprises fiber layers and hydrogel layers, wherein the fiber layers and the hydrogel layers are arranged in an alternating mode in the space, fibers of the fiber layers are arranged in an ordered or disordered mode, and cells are contained or not contained in the hydrogel layers. The 3D printing forming device comprises a scanning imaging system, a rapid forming system, a transmission system and a control system. The tough tissue structure can simulate the composite state of cells, matrixes and fibers of tough tissue in a human body in the aspects of mechanics, morphology and biology, and can be used for direct repair and regeneration of the tough tissue at the parts such as achilles tendons, ligaments, urethras and gynecology pelvic floor supporting systems. According to the tough tissue structure and the 3D printing forming device and method of the tough tissue structure, direct combination formation of the fibers, the cells and hydrogel in vitro and vivo is achieved, and the purpose that the tough tissue with lesions can be directly printed, regenerated or replaced in vitro and vivo during clinical surgery can be achieved.

Description

technical field [0001] The invention belongs to the field of tissue engineering, composite materials and medical devices, and relates to a tough tissue structure and its 3D printing forming equipment and method. Background technique [0002] At present, the injury treatment and repair of tough tissues (Achilles tendon, fascia, ligament, urethra and gynecological pelvic floor support system, etc.) mainly rely on bionic material replacement, autograft or allograft, but the biocompatibility The sex is not high, the injury recovery is slow, and even faces the problems of immune rejection and virus infection, and the treatment and repair of adults are more difficult than that of children. Regenerative medicine and tissue engineering provide the possibility for the repair and reconstruction of human connective tissue, which involves biology, materials science, mechanics and other disciplines. [0003] Tough tissue, such as connective tissue, is one of the basic tissues of humans ...

Claims

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Application Information

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IPC IPC(8): A61F2/08A61L27/18A61L27/22A61L27/24A61L27/38A61L27/52
CPCA61F2/08A61F2210/0076A61F2240/002B33Y70/00
Inventor 王小红许雨帆
Owner TSINGHUA UNIV
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