Method for improving bacitracin valence through continuous flowing fed-batch fermentation

Abstract

The invention discloses a method for improving the bacitracin valence through continuous flowing fed-batch fermentation. The method comprises the following steps: a, performing slant culture: inoculating bacillus licheniformis into a slant culture medium, and culturing for 24 hours at 37 DEG C; b, preparing bacterium suspension: preparing the slant strain prepared in the step a into bacterium suspension by using sterile water; c, performing one-class seed culture: inoculating the bacterium suspension prepared in the step b into a one-class seed jog with a seed culture medium, and culturing for 16 hours under the conditions that the temperature is 37 DEG C, the rotation speed is 600rpm and the gas supply amount is 0.5vvm; d, performing fed-batch fermentation: inoculating the one-class seeds prepared in the step c into a fermentation tank with a fermentation culture medium according to a volume ratio of 10%, fermenting under the conditions that the temperature is 37 DEG C, the rotation speed is 600rpm and the gas supply amount is 1.0vvm, when the strain enters into an index growth period, starting supplementing materials at uniform speed, after the supplementation, controlling the pH value to be 7.0-7.5, and stopping fermentation after 40 hours; and e, detecting fermentation unit: detecting the fermentation unit of bacitracin, and comparing the fermentation unit of bacitracin with the fermentation unit of the bacitracin without fed-batch fermentation in production.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for increasing the potency of bacitracin by continuous feeding and fed-batch fermentation. Background technique [0002] Bacitracin is a polypeptide antibiotic, which is an unstable polypeptide composed of a variety of amino acids. It has a strong inhibitory effect on Gram-positive bacteria and some negative bacteria. It can inhibit the synthesis of bacterial cell walls, affect their protein synthesis and Cell membrane function, so as to achieve the purpose of eliminating pathogenic bacteria. Composed of multiple amino acids, bacitracin is widely used in livestock and poultry farming due to its high efficiency, no toxic side effects, no residue, no drug resistance and cross-resistance, and fast excretion. [0003] Bacillus licheniformis is used in the fermentation production of bacitracin, with corn starch and soybean meal as the main carbon and nitroge...

AI Technical Summary

Problems solved by technology

[0003] Bacillus licheniformis is used in the fermentation production of bacitracin, with corn starch and soybean meal as the main carbon and nitrogen sources. The cost is high. At present, the research on the production of bacitracin by microbial fermentation is mainly the optimization of the fermentation medium or the optimization of the fermentation conditions, and no good results have been achieved; some factories also use traditional mutation breeding to screen high-yield bacitracin. Strains, although the fermentation unit has been improved to a certain extent, but the vigor of the strains is constantly declining