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Cordyceps cicadae extract and application thereof in preparing nerve protecting and anti-aging medicaments

A technology for neuroprotection and Cicada japonica, applied in the field of medicine, can solve the problems that no Cicada japonica extract has been refined, and the active functional components are not very clear.

Active Publication Date: 2014-05-21
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the fact that cicadae and the anamorph of the famous and precious Chinese medicine Cordyceps sinensis belong to fungi (Cordyceps genus), and Paecilomyces cicadae has various pharmacological effects such as immunity, and has the advantages of low toxicity and easy cultivation, it is hopeful that it can be used as a substitute for Cordyceps sinensis. Its corresponding active functional components are not very clear, and most of the pharmacological experiments only stay on the basis of the original medicinal materials or crude extracts, and there is no report that the extract of Cicada japonica is further refined to the active site and used to prepare neuroprotective and anti-aging drugs

Method used

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  • Cordyceps cicadae extract and application thereof in preparing nerve protecting and anti-aging medicaments
  • Cordyceps cicadae extract and application thereof in preparing nerve protecting and anti-aging medicaments
  • Cordyceps cicadae extract and application thereof in preparing nerve protecting and anti-aging medicaments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The water-soluble part (JCH H2O )preparation:

[0017] Take 80 g of dried cicadae powder that has been crushed through the Pharmacopoeia No. 2 sieve, add 10 times of 80% ethanol for reflux extraction, and filter to recover ethanol. Cicada ethanol extract was dispersed with water, followed by fractional extraction with equal volumes of petroleum ether, ethyl acetate, and n-butanol, extracted 3 times in total, combined, concentrated under reduced pressure to recover the solvent, and respectively obtained petroleum ether fractions, ethyl acetate fractions, The n-butanol part, the water part, and the water part are concentrated, passed through AB-8 macroporous adsorption resin, washed with water until nearly colorless, and then eluted with 3BV80% (v / v) ethanol; collected 80% ethanol eluate, concentrated , freeze-dried to get JCH H2O 4.24g.

Embodiment 2

[0018] Example 2JCH H2O Protective effect on glutamate-induced senescence of PC12 cells

[0019] (1) Determination of cell viability by MTT method and LDH method:

[0020] Take PC12 cells in the logarithmic growth phase (provided by Jiangsu University School of Medicine), and use 2 × 10 4 Inoculate / well in 96-well culture plate, 200 μl / L. At 37°C, 5% CO 2 After culturing overnight under the condition, the cells were divided into control group, model group and drug treatment group. That is, the control group (complete medium without Glu); model group (complete medium + Glu); drug treatment group (complete medium + JCH H2O +Glu, the concentrations were 200μg / mL, 100μg / mL, and 50μg / mL, diluted with culture medium. )JCH H2O After pretreatment for 1 h, add Glu and incubate for 24 h. Set up 4 parallel wells in each group. After culturing for 24 hours, add 20 μl of 5g / L MTT to each well, continue culturing for 4 hours, then terminate the culture, carefully suck the supernatant...

Embodiment 3

[0037] Example 3JCH H2O In vitro antioxidant activity assay:

[0038] (1) Determination of DPPH free radical scavenging ability:

[0039] Add 1.0mL of 200μmol / DPPH ethanol solution to 1mL of Cicada flower extract solution with different concentrations, then add 2.0mL of 80% ethanol and mix evenly. After placing it in a dark place for 30min, measure the absorbance value A at 517nm with a UV spectrophotometer 样品 , and simultaneously measure the absorbance value A of the mixture of 1.0mL DPPH ethanol solution and 3.omL ethanol solution 空白 and the absorbance value A of the mixture of 3mL ethanol and 1.0mL sample 对照 , clearance rate formula: DPPH clearance rate=[A 空白 -(A 样品 -A 对照 ) / A 空白 ]×100%. The data show that JCH H2O Has the ability to remove DPPH·. The results are shown in Table 5

[0040] (2) Superoxide anion (O 2·- ) Determination of free radical scavenging ability:

[0041] Add 3ml Tris-HCl (PH8.2) to 1mL different concentrations of Cicada flower extract solutio...

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Abstract

The invention relates to a Cordyceps cicadae extract and application thereof in preparing nerve protecting and anti-aging medicaments. The extract is a Cordyceps cicadae ethanol extraction water soluble part and is prepared by the steps of pulverizing Cordyceps cicadae, sieving, performing reflux extraction with ethanol, dispersing ethanol extract in water, performing gradient extraction with petroleum ether, ethyl acetate and n-butanol in sequence, concentrating under reduced pressure to recover solvent, freeze-drying to obtain a petroleum ether part, an ethyl acetate parts, a n-butanol part and a water part, and concentrating the water part; separating with AB-8 macroporous adsorption resin, washing with water to nearly colorless, and eluting with 80% ethanol; collecting ethanol eluate, concentrating, and freeze-drying to obtain water soluble extract (JCHH2O). The extract provided by the invention can remarkably inhibit glutamic acid-induced aging injury of PC12 cells, and reduce intracellular oxygen radical level, and has in vitro antioxidant activity in experiments of scavenging DPPH. and superoxide anion (O<2.->) free radicals.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the preparation of parts with neuroprotective and anti-aging activities from Cicada japonica, a Chinese medicinal material, and its application in the preparation of neuroprotective and anti-aging drugs. Background technique [0002] With the aging population in our country, the incidence of neurodegenerative diseases is increasing day by day, and the research on aging and neurodegenerative diseases has become a neuroscience hotspot. The aging of the human body often leads to an accelerated reduction in the number of nerve cells, resulting in some neurodegenerative diseases represented by Alzheimer's Disease (AD). At present, the etiology of neurodegenerative diseases is unknown, and there is no effective treatment. Only a few drugs can be used to treat neurodegenerative diseases. [0003] Cicada is a dry complex formed by the fungus Cordyceps cicadae Shing of the f...

Claims

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Application Information

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IPC IPC(8): A61K36/068A61P25/00A61P39/06A61P25/28
Inventor 欧阳臻张魏琬麒赵明王吉标尚磊王璠汪愿
Owner JIANGSU UNIV
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