Preparation method of high-intensity anti-ultraviolet silk fibroin film

A silk fibroin and anti-ultraviolet technology, applied in the fields of protein chemistry, material science, and biochemistry, can solve the problem of silk fibroin film brittleness, decreased biocompatibility, softness, elongation and elasticity of silk fibroin film and other problems, to achieve the effect of increased anti-ultraviolet ability, simple preparation process, and improved anti-ultraviolet ability

Inactive Publication Date: 2014-05-14
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above methods can improve the mechanical properties of silk fibroin films to varying degrees, the introduction of foreign species will result in uneven degradation of the obtained silk fibroin films and a decrease in biocompatibility, which affects its application range. Harsh process conditions, easy to produce harmful chemical residues and environmental pollution
It seems that the existing production process is difficult to produce silk fibroin film with good mechanical properties
The main reason for the high brittleness and poor mechanical properties of the silk fibroin film is that the C-N bond length of the peptide bond -CO-NH- on the silk fibroin protein macromolecular peptide chain is 0.132nm, which is 0.147nm longer than the C-N sing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Take 500g of 1-butyl-3-methylimidazolium acetate ionic liquid, slowly add 88.2g of silk fibroin obtained by degumming domestic mulberry silk at 75°C, its water content is 8.5%, ash content is 0.5%, and stir for 3 hours. The silk fibroin is completely dissolved to obtain a silk fibroin solution with a mass concentration of 15 wt%. The solution was filtered twice with a 300-mesh filter cloth, and stood overnight to obtain a colorless and transparent silk fibroin film-forming stock solution. Spread the stock solution on a 4cm×10cm glass plate, then scrape it flat with a glass rod, and solidify it in absolute ethanol at 25°C to form a film. The obtained membrane was washed several times in deionized water until the membrane layer was transparent and colorless. Take out the silk fibroin film, place it overnight at room temperature, let it dry naturally (about 12 hours), and finally obtain a transparent silk fibroin film, the breaking strength of the silk fibroin film is 17M...

Embodiment 2

[0016] Take 500g of 1-ethyl-3-methylimidazolium acetate ionic liquid, slowly add 88.2g of silk fibroin obtained by degumming domestic mulberry silk at 75°C, its water content is 8.5%, ash content is 0.5%, and stir for 3 hours. The silk fibroin is completely dissolved to obtain a silk fibroin solution with a mass concentration of 15 wt%. The solution was filtered twice with a 300-mesh filter cloth, and stood overnight to obtain a colorless and transparent silk fibroin film-forming stock solution. Spread the stock solution on a 4cm×10cm glass plate, then scrape it flat with a glass rod, and solidify it in absolute ethanol at 25°C to form a film. The obtained membrane was washed several times in deionized water until the membrane layer was transparent and colorless. Take out the silk fibroin film, place it overnight at room temperature, let it dry naturally (about 12h), and finally obtain a transparent silk fibroin film, the breaking strength of the silk fibroin film is 16Mpa, t...

Embodiment 3

[0018] Take 500g of 1-butyl-3-methylimidazolium acetate ionic liquid, slowly add 68.2g of silk fibroin obtained by degumming domestic mulberry silk at 75°C, the water content is 8.5%, the ash content is 0.5%, and stir for 3 hours. The silk fibroin is completely dissolved to obtain a silk fibroin solution with a mass concentration of 12 wt%. The solution was filtered twice with a 300-mesh filter cloth, and stood overnight to obtain a colorless and transparent silk fibroin film-forming stock solution. Spread the stock solution on a 4cm×10cm glass plate, then scrape it flat with a glass rod, and solidify it in absolute ethanol at 25°C to form a film. The obtained membrane was washed several times in deionized water until the membrane layer was transparent and colorless. Take out the silk fibroin film, place it overnight at room temperature, let it dry naturally (about 12h), and finally obtain a transparent silk fibroin film, the breaking strength of the silk fibroin film is 16Mp...

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Abstract

The invention discloses a preparation method of high-intensity anti-ultraviolet silk fibroin film. The preparation method comprises the following steps: dissolving degummed mulberry silk in an imidazole carboxylate ion liquor to prepare a 10-15wt% silk fibroin solution, performing filter pressing and static defoaming, then naturally spreading the silk fibroin solution on a glass plate by adopting a tape casting method, soaking the glass plate in an absolute ethyl alcohol solution for condensation, washing with water at normal temperature, and naturally drying to obtain the silk fibroin film. The breaking strength of the silk fibroin film is larger than 15Mpa, the elongation at beak is larger than 20%, and the breaking strength of the film can be over 12Mpa after the film is irradiated under an ultraviolet lamp. The film is nontoxic and harmless, has a good biocompatibility and a characteristic of high intensity, and can be used as surgical restorative auxiliary materials such as an artificial skin material and a drug slow-release carrier. A preparation process is simple, the solution can be recycled, and the silk fibroin film is environment-friendly and has good application prospect.

Description

technical field [0001] The invention belongs to the fields of material science, biochemistry and protein chemistry, and in particular relates to a preparation method of a high-strength silk fibroin film. Specifically, degummed mulberry silk is used as raw material and imidazole carboxylate ionic liquid is used as solvent to prepare a suitable film-forming stock solution; after coagulation, washing, drying and other processes, a high-strength anti-ultraviolet silk fibroin film is prepared. Background technique [0002] Silk fibroin is the main component of natural silk, which accounts for 70-80% of the silk, and is obtained by degumming silk. Silk fibroin has good biocompatibility and degradability with the human body, and has a wide range of applications in the biological field. Among them, silk fibroin film is the earliest and most deeply studied silk fibroin material. It is obtained by drying silk fibroin solution. Its biggest disadvantage is its high brittleness and poor...

Claims

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Application Information

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IPC IPC(8): C08J5/18C08J3/09C08L89/00
Inventor 李露于世涛刘仕伟解从霞任厚朋
Owner QINGDAO UNIV OF SCI & TECH
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