Preparation method of surface imprinting adsorbent for identifying ciprofloxacin at high sensitivity

A technology of ciprofloxacin and surface imprinting, applied in chemical instruments and methods, and other chemical processes, can solve the problem of poor adsorption-desorption kinetics, deep embedding of active sites, mass transfer and charge transfer Kinetic rate is slow and other problems, to achieve significant separation effect, reduce non-specific adsorption, low price effect

Inactive Publication Date: 2014-05-14
JIANGSU UNIV
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Problems solved by technology

[0003] Surface molecular imprinting technology builds molecular recognition sites on the surface of matrix materials, which facilitates the removal and recombination of template molecules, and overcomes some serious defects in the bulk polymerization method, such as too deep embedding of active sites, The kinetic rate of mass transfer and charge transfer is slow, the kinetic performance of adsorption-desorption is not good, etc.

Method used

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  • Preparation method of surface imprinting adsorbent for identifying ciprofloxacin at high sensitivity
  • Preparation method of surface imprinting adsorbent for identifying ciprofloxacin at high sensitivity
  • Preparation method of surface imprinting adsorbent for identifying ciprofloxacin at high sensitivity

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Embodiment 1

[0031] (1) Preparation of ciprofloxacin surface imprinted polymers (MIPs)

[0032] Put 0.2 g of active dry yeast into a 100 ml round-bottomed flask, add 10 ml of water, mix until the yeast dissolves, then add 40 ml of acetonitrile, sonicate for 10 min, then add ciprofloxacin 0.0625 mmol, acrylamide 0.25 mmol, 1 mmol of ethylene glycol bis(methacrylate) ester, mix and stir, pass nitrogen for 15 min, then carefully add 0.0043 g of 2,2'-azobisisobutyronitrile, seal the bottle mouth, and put it in a water bath In the shaker, set the temperature at 50°C and rotate at 180 rpm, react for 2 hours, then increase the temperature to 60°C, and continue the reaction for 22 hours. Then centrifuge and dry under vacuum at 60°C for 12 h. The synthesized product was Soxhlet extracted with a mixture of 90 mL methanol and 10 mL acetic acid for 48 h to remove the template molecule ciprofloxacin. Finally, ciprofloxacin surface-imprinted polymer was obtained by vacuum drying at 50°C for 6 h.

[0...

Embodiment 2

[0042] (1) Preparation of ciprofloxacin surface imprinted polymers (MIPs)

[0043] Put 0.15 g of active dry yeast into a 100 ml round bottom flask, add 10.5 ml of water, mix until the yeast dissolves, then add 42 ml of acetonitrile, ultrasonicate for 10 min, then add ciprofloxacin 0.05 mmol, acrylamide 0.3 mmol, 1.5 mmol of ethylene glycol bis(methacrylate) ester, mix and stir, pass nitrogen for 15 min, then carefully add 0.0096 g of 2,2'-azobisisobutyronitrile, seal the bottle mouth, and put it in a water bath In the shaker, set the temperature at 50°C, rotate at 180 rpm, react for 2 h, and increase the temperature to 60 oC , continue to react for 22 h. Then centrifuge and dry under vacuum at 60°C for 12 h. The synthesized product was Soxhlet extracted with a mixture of 90 mL methanol and 10 mL acetic acid for 48 h to remove the template molecule ciprofloxacin. Finally, ciprofloxacin surface-imprinted polymer was obtained by vacuum drying at 50°C for 6 h.

[0044] The c...

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Abstract

The invention relates to a preparation method of a surface imprinting adsorbent for identifying ciprofloxacin at high sensitivity and belongs to the technical field of preparing environmental materials, and in particular relates to a method for preparing a surface imprinting adsorbent of ciprofloxacin with active dry yeast as the substrate, ciprofloxacin (CIP) as the template molecule, acrylamide (AM) as the functional monomer, ethylene glycol dimethyl acrylate (EGDMA) as the cross-linking agent and 2,2'-azodiisobutyronitrile (AIBN) as the initiator through a precipitation polymerization process. Static adsorption experiment is carried out to study the adsorption balance, dynamics and high-selectivity identification properties of the prepared imprinting adsorbent, and the result of the static adsorption experiment indicates that the surface imprinting adsorbent has fast adsorption dynamic property and excellent ciprofloxacin molecule identification property.

Description

technical field [0001] The invention relates to a surface imprint preparation method for highly selective recognition of ciprofloxacin, which belongs to the technical field of environmental material preparation. Background technique [0002] Molecular Imprinting Technique (MIT) refers to a technology that uses target molecules as template molecules to prepare Molecularly Imprinted Polymers (MIPs) with specific and selective recognition functions for the molecules. The reason why molecular imprinting technology has attracted the attention of many scientific workers is mainly because it has three characteristics: namely, predeterminability, specific recognition, and wide applicability. Predeterminability means that people can prepare molecularly imprinted polymers with different template molecules according to different needs to meet various requirements; specific recognition is because molecularly imprinted polymers are customized according to template molecules, which have u...

Claims

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Application Information

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IPC IPC(8): C08F220/56C08F222/14C08J9/26C08J9/28B01J20/26B01J20/30
Inventor 戴江栋王娟曹志晶孟敏佳李春香闫永胜
Owner JIANGSU UNIV
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