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Nano material for detecting dimethoate and preparation method thereof

A technology of nanomaterials and dimethoate, which is applied in the measurement of color/spectral characteristics, etc., to achieve the effects of high specific surface area, stable structure, and increased surface effect

Active Publication Date: 2014-05-07
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of nanometer material and preparation method thereof that detects dimethoate for the deficiencies of existing biological enzyme detection materials, have production cost low, can realize the rapid, selective detection of dimethoate, and detect The process does not require other reagents other than the sample, etc.

Method used

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  • Nano material for detecting dimethoate and preparation method thereof
  • Nano material for detecting dimethoate and preparation method thereof
  • Nano material for detecting dimethoate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The composition and weight percent of the nanometer material of the detection dimethoate provided by the present embodiment are:

[0037] Sodium silicate: 97.43%

[0038] TPPS 4 : 0.23%

[0039] Plant esterase: 2.34%

[0040] The specific method steps of its preparation method are as follows:

[0041] 1) Preparation of plant esterase enzyme solution

[0042] Using commercially available flour as raw material, according to the weight ratio of flour:distilled water as 1:6, add distilled water to the flour, stir for 10 minutes, then centrifuge at a centrifugal speed of 3000r / min for 10 minutes, collect the supernatant, and statically After placing it for 8 hours, the plant esterase enzyme liquid is prepared;

[0043] 2) Preparation of sol solution

[0044] According to the weight ratio of sodium silicate: distilled water is 1:4, distilled water is added to sodium silicate, and the pH of the system is adjusted to 5 with hydrochloric acid, and after ultrasonic mixing f...

Embodiment 2

[0052] The composition and weight percent of the nanometer material of the detection dimethoate provided by the present embodiment are:

[0053] Sodium silicate: 99.31%

[0054] TPPS 4 : 0.06%

[0055] Plant esterase: 0.63%

[0056] The specific method steps of its preparation method are as follows:

[0057] 1) Preparation of plant esterase enzyme solution

[0058] Using commercially available flour as raw material, according to the weight ratio of flour:distilled water as 1:4, add distilled water to the flour, stir for 60min, then centrifuge for 15min at a centrifugal speed of 6000r / min, collect the supernatant, and statically After leaving it for 24 hours, the plant esterase enzyme liquid is prepared;

[0059] 2) Preparation of sol solution

[0060] According to the weight ratio of sodium silicate: distilled water is 1:3.17, distilled water is added to sodium silicate, and the pH of the system is adjusted to 4 with hydrochloric acid, and after ultrasonic mixing for 40s...

Embodiment 3

[0068] The composition and weight percent of the nanometer material of the detection dimethoate provided by the present embodiment are:

[0069] Sodium silicate: 98.51%

[0070] TPPS 4 : 0.13%

[0071] Plant esterase: 1.36%

[0072] The specific method steps of its preparation method are as follows:

[0073] 1) Preparation of plant esterase enzyme solution

[0074] Using commercially available flour as raw material, according to the weight ratio of flour:distilled water as 1:5, add distilled water to the flour, stir for 30min, then centrifuge at a centrifugal speed of 4000r / min for 12min, collect the supernatant, and statically Leave it for 12 hours, and the plant esterase enzyme liquid is prepared;

[0075] 2) Preparation of sol solution

[0076] According to the weight ratio of sodium silicate: distilled water is 1:3.45, distilled water is added to sodium silicate, and the pH of the system is adjusted to 4 with hydrochloric acid, and after ultrasonic mixing for 30s, th...

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Abstract

The invention aims at providing a nano material for detecting dimethoate. The nano material comprises the following components by weight percent: 97.43 to 99.31 percent of sodium silicate, 0.06 to 0.23 percent of TPPS4 and 0.63 to 2.34 percent of plant-esterase. The plant-esterase is fixed by adopting a sol-gel technology, the fixation process is a physical embedding process, a gel network is gradually formed surrounding the enzyme, the structure completeness and isotropy and biological activity of surface microstructure of the enzyme can be maintained by virtue of the moderate reaction condition, amorphous structure of the gel and biological compatibility. Then the TPPS4 enters the gel network by virtue of the micropores on the surface of the gel to be reacted with the plant-esterase so as to form aggregation on the surface of the enzyme. In the gel network, the enzyme and the aggregation have less possibility of being subjected to the unfavorable influence of unfavorable environmental factors, but also the structure is more stable, the precipitate is unlikely to form, the enzyme and the aggregation are easy to store, the reaction activity can be well protected, and the detection reaction is favored.

Description

technical field [0001] The invention belongs to the technical field of materials for detecting organophosphorus pesticides and the preparation thereof, in particular to a nanometer material for detecting dimethoate and a preparation method thereof. Background technique [0002] Dimethoate is an organophosphorus systemic insecticide with contact and stomach poisoning, its chemical name is O, O-dimethyl-S-(N-methylcarbamoylmethyl) dimethoate Phosphorothioate. Dimethoate is an insecticide with a wide range of uses and a large yield. It has a good control effect on piercing-sucking mouthparts pests, such as aphids and whiteflies. However, dimethoate is volatile, and a large amount of lost pesticides volatilize into the air, water body or settle and gather in the soil to pollute agricultural, livestock and fishery by-products, and transfer to the human body through the enrichment of the food chain, causing harm to the human body. Studies have shown that dimethoate has potential...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 杨丽敏姜磊刘振李友训
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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