Storage and reactivation methods of anaerobic ammonia oxidation granule sludge

Abstract

The invention provides a storage method of anaerobic ammonia oxidation granule sludge. The storage method comprises the steps of collecting anaerobic ammonia oxidation granule sludge to be stored; firstly, washing the anaerobic ammonia oxidation granule sludge for 3-5 times by using an inorganic salt buffer solution before storage; selecting a clean organic plastic bottle with good air tightness as a storage container; firstly, adding clean water and dimethyl sulfoxide to the storage container, and putting the storage container into an environment at 0-4 DEG C for refrigerating for 3-6 hours, so as to transfer the cleaned sludge to the refrigerated sealed container; placing the sealed container in the environment at 0-4 DEG C to store after aerating for 20-30 minutes by argon. The invention also provides a reactivation method of stored anaerobic ammonia oxidation granule sludge. The reactivation method comprises the steps of taking out the storage container; firstly, carrying out a temperature adaptation test before activity recovery; cleaning the stored granule sludge for 6-10 times by using the inorganic salt buffer solution after temperature adaptation, and then transferring to an anaerobic reactor to be started to carry out activity recovery. By adopting the methods, the anaerobic ammonia oxidation granule sludge can be successfully stored, and the activity of the granule sludge is quick to recover, so that the subsequent use is convenient.

Description

Technical field [0001] The invention relates to a storage and reactivation method of anaerobic ammonia oxidation granular sludge. Background technique [0002] The anammox process has attracted much attention because of its high efficiency and energy saving. Anammox bacteria, as the core carrier of the anammox reaction, have been discovered in industrial wastewater treatment plants since the 1990s, and have further advanced theoretical research into practical applications. However, anammox bacteria have a long doubling time and slow growth, so it is difficult to apply to actual wastewater treatment. Enrichment, cultivation and effective preservation of anammox bacteria are effective measures to break through the bottleneck of application. [0003] Common methods of preservation of bacteria include liquid paraffin method, liquid nitrogen method, freezing method, freeze-drying method and gel embedding method. Although these technologies can effectively retain bacterial activity to...

AI Technical Summary

Problems solved by technology

Although these technologies can effectively retain bacterial activity to a certain extent, if they are applied to the preservation process of anaerobic ammonium oxidation granular sludge, there will inevitably be certain disadvantages, such as affecting the morphology of granular sludge and causing sludge structure. Variation, complex operation process and high technical requirements