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A kind of α-hed-cs-cd147 nanoparticle and its preparation method, application and preparation

A -hed-cs-cd147, CD147 technology, applied in the field of pharmaceutical preparations, can solve the problems of poor water solubility of α-hedera saponin, affecting application and research, and low bioavailability, so as to increase selectivity and improve relative bioavailability in vivo Utilization, the effect of enhancing the therapeutic effect

Active Publication Date: 2016-08-31
JIANGXI HERBFINE HI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor water solubility of α-hedera saponin, it can only be administered by intragastric administration in animal experiments, resulting in low bioavailability, and difficulty in intraperitoneal and intravenous administration, which affects its further application and research.

Method used

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  • A kind of α-hed-cs-cd147 nanoparticle and its preparation method, application and preparation
  • A kind of α-hed-cs-cd147 nanoparticle and its preparation method, application and preparation
  • A kind of α-hed-cs-cd147 nanoparticle and its preparation method, application and preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Preparation of Example 1α-Hed-CS-CD147 Nanoparticles

[0061] Accurately weighed 80mg F68 and 50mg CS, dissolved in 20mL of 0.2% acetic acid aqueous solution as the aqueous phase; accurately weighed 20mgα-Hed and 80mg phospholipids, dissolved in 4mL ethanol / acetone (1:1) mixed solvent as the organic phase, in Under the condition of magnetic stirring at 30°C, quickly inject the organic phase into the water phase, keep stirring for 5 minutes, then dilute to 70mL with distilled water, and cut it with a high-speed shearer at 20000rpm for 5min, and then homogenize it with a high-pressure homogenizer at 1000bar. After massaging for 5 minutes, filter through a 0.45 μm microporous membrane to obtain α-Hed-CS-NP (α-hedera-CS nanoparticles) colloidal solution.

[0062] The α-Hed-CS-NP colloidal solution prepared above was concentrated to 1 / 10 of the original volume, and the residual organic solvent was removed by ultracentrifugation. The precipitate was washed twice and then resu...

Embodiment 2

[0063] Preparation of Example 2α-Hed-CS-CD147 Nanoparticles

[0064] Accurately weighed 80mg F68 and 50mg CS, dissolved in 20mL of 0.2% acetic acid aqueous solution as the aqueous phase; accurately weighed 20mgα-Hed and 80mg phospholipids dissolved in 4mL ethanol as the organic phase, under the condition of magnetic stirring at 30°C, the organic Inject the phase into the water phase quickly, keep stirring for 10min, then dilute to 70mL with distilled water, and cut it with a high-speed shearer at 20000rpm for 5min, then homogenize it at 1000bar for 5min with a high-pressure homogenizer, and pass through a 0.45μm microporous filter Membrane filtration to obtain α-Hed-CS-NP (α-hedera-CS nanoparticles) colloidal solution.

[0065] The α-Hed-CS-NP colloidal solution prepared above was concentrated to 1 / 10 of the original volume, and the residual organic solvent was removed by ultracentrifugation. The precipitate was washed twice and then resuspended with 0.2% acetic acid solution....

Embodiment 3

[0066] Preparation of Example 3α-Hed-CS-CD147 Nanoparticles

[0067] Accurately weighed 80mg F68 and 50mg CS, dissolved in 20mL of 0.2% acetic acid aqueous solution as the aqueous phase; accurately weighed 20mgα-Hed and 80mg phospholipids dissolved in 2mL acetone as the organic phase, under the condition of magnetic stirring at 30°C, the organic Inject the phase into the water phase quickly, keep stirring for 5min, and then dilute to 70mL with distilled water, while shearing with a high-speed shearer at 20000rpm for 5min, then homogenize for 5min at 1000bar with a high-pressure homogenizer, and pass through a 0.45μm microporous filter Membrane filtration to obtain α-Hed-CS-NP (α-hedera-CS nanoparticles) colloidal solution.

[0068] The α-Hed-CS-NP colloidal solution prepared above was concentrated to 1 / 10 of the original volume, and the residual organic solvent was removed by ultracentrifugation. The precipitate was washed twice and then resuspended with 0.2% acetic acid solut...

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Abstract

The invention relates to the field of pharmaceutical preparations and in particular relates to alpha-Hed-CS-CD147 nanoparticles as well as a preparation method, an application and a preparation thereof. The alpha-Hed-CS-CD147 nanoparticles comprise alpha-Hed, CD147 monoclonal antibodies, CS (chitosan) and phospholipid. The nanoparticles provided by the invention are uniform in particle size distribution, can better retain the antibody activity, have passive and active targeting effects, improve the in-vivo bioavailability of drugs and have a better anti-liver-tumor effect.

Description

technical field [0001] The invention relates to the field of pharmaceutical preparations, in particular to α-Hed-CS-CD147 nanoparticle and its preparation method, application and preparation. Background technique [0002] Liver cancer refers to malignant tumors that occur in the liver, and its mortality rate ranks third among malignant tumors. According to the statistics of the World Health Organization, the incidence of primary liver cancer is on the rise all over the world, and nearly 50% of the new cases appear in China. Therefore, how to prevent and treat liver cancer has become a hot spot in modern medical research. [0003] In recent years, the research on the anti-tumor active components of traditional Chinese medicine has achieved fruitful results, and a variety of chemical components with anti-tumor effects have been successfully isolated from traditional Chinese medicine. α-hedera saponin (α-Hed) is the active ingredient extracted from the traditional Chinese med...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/48A61K47/36A61K47/24A61K9/00A61K31/704A61P35/00A61P1/16
Inventor 杨世林游本刚冯育林许琼明刘艳丽李笑然
Owner JIANGXI HERBFINE HI TECH
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