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Method for preparing epothilone molecularly imprinted polymer film by adopting mixed template

A technology of epothilone and molecular imprinting, applied in the directions of alkali metal compounds, chemical instruments and methods, membrane technology, etc., to achieve the effect of overcoming the need for sieving, easy control of reaction conditions, and shortening of elution time

Active Publication Date: 2014-04-16
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the preparation of epothilone molecularly imprinted polymer membrane and its application in the fermentation production and separation and extraction process have not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method for preparing an epothilone molecularly imprinted polymer film by using a mixed template comprises the following steps:

[0027] 1) Mix 1.0mmol of epothilone B and 1.0mmol of epothilone D as the template molecule, add 4mmol of functional monomer acrylic acid (AA) to form a host-guest complex, and then add 20mmol of cross-linking agent ethylene glycol dimethyl Acrylic acid ester (EGDMA), fully mixed to form a mixture, the mixture was dissolved in 40mL porogen acetonitrile, at an ultrasonic frequency of 60000Hz, ultrasonic treatment for 10min to make it evenly mixed, let stand at room temperature for 1h, and then added 20mg initiator azobisisobutyronitrile, mixed to obtain a mixed solution;

[0028] 2) Soak the cellulose acetate membrane in 30% N,N,N',N'-tetramethylethylenediamine aqueous solution for 0.5h, then take it out and dry it in a vacuum oven at 30°C for 4h, and set aside ;

[0029] 3) Put the cellulose acetate membrane treated in step 2) into the mi...

Embodiment 2

[0031] The method for preparing an epothilone molecularly imprinted polymer film by using a mixed template comprises the following steps:

[0032] 1) Mix 1.0mmol epothilone B and 1.5mmol epothilone D as template molecules, add 7.5mmol functional monomer methyl methacrylate (MA) to form a host-guest complex, and then add 25mmol cross-linking agent Methylenebisacrylamide, mix thoroughly to form a mixture; dissolve the mixture in 50mL of porogen chloroform, at an ultrasonic frequency of 50000Hz, ultrasonic treatment for 15min to make it evenly mixed, let it stand at room temperature for 2h, then add 30mg initiator azobisisobutyronitrile, mixed to obtain a mixed solution;

[0033] 2) Soak the polyethylene film in a 40% (v / v) N, N, N', N'-tetramethylethylenediamine aqueous solution for 1 hour, then take it out and dry it in a vacuum oven at 40°C for 3 hours ,spare;

[0034] 3) Then put the polyethylene film treated in step 2) into the mixed solution prepared in step 1) and soak f...

Embodiment 3

[0036] The method for preparing an epothilone molecularly imprinted polymer film by using a mixed template comprises the following steps:

[0037] 1) Mix 1.0mmol epothilone B and 2.0mmol epothilone D as template molecules, add 9mmol functional monomer styrene to form a host-guest complex, and then add 60mmol cross-linking agent trimethylpropanetrimethyl Acrylic ester (TRIM), mix well to form a mixture; dissolve the mixture in 66mL of porogen carbon tetrachloride, at an ultrasonic frequency of 30000Hz, sonicate for 20min to make it evenly mixed, and let it stand at room temperature for 1h. Then add 35mg initiator azobisisobutyronitrile, mix to obtain mixed solution;

[0038] 2) Soak the nylon membrane in 50% (v / v) N,N,N',N'-tetramethylethylenediamine aqueous solution for 1h, then take it out and dry it in a vacuum oven at 50°C for 2.5h ,spare;

[0039] 3) Put the nylon membrane treated in step 2) into the mixed solution prepared in step 1) and soak for 25 minutes, take it out...

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Abstract

The invention discloses a method for preparing an epothilone molecularly imprinted polymer film by adopting a mixed template and belongs to the technical field of molecular imprinting. The method comprises the following steps: 1, after mixing template molecule epothilone B and epothilone D, adding a functional monomer and a cross-linking agent, sufficiently and uniformly mixing to obtain a mixture, adding the mixture into a pore-foaming agent, uniformly mixing under the ultrasonic condition, standing at room temperature, then adding an initiator and uniformly mixing to obtain mixed liquid; 2, after soaking a matrix film in the mixed liquid, taking out the matrix film, placing the matrix film between two glass sheets, removing air bubbles, placing the glass sheets in a sealed bag, filling nitrogen, sealing up the bag, irradiating the bag by an ultraviolet lamp, separating the glass sheets to obtain the epothilone molecularly imprinted polymer film. The method is easy to operate; the reaction conditions are easy to control; the preparation cost is low; the film is easy to carry out readsorption and elution and has a high repeated utilization rate; not only are the defects of long polymerization time and requirement for screening of a rodlike molecularly imprinted polymer overcome, but also elution time is shortened.

Description

technical field [0001] The invention belongs to the technical field of molecular imprinting, and in particular relates to a method for preparing an epothilone molecular imprinting polymer film by using a mixed template. Background technique [0002] Epothilone is a class of macrolide compounds produced by Sorangium cellulosum, which has a variety of natural derivatives, has the same mechanism of action as paclitaxel in inhibiting tumor cell growth, and is resistant to multiple drugs Both tumor cells and paclitaxel-resistant tumor cells exhibit strong anticancer activity. With the deepening of research, epothilone will eventually play a huge role in the treatment of human cancer. [0003] At present, there have been some related studies on the fermentation production and separation and extraction of epothilone, but the existing fermentation production methods generally have problems such as unstable yield and low yield, and the separation and extraction steps are cumbersome a...

Claims

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Application Information

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IPC IPC(8): C08J9/28C08J7/18B01J20/26B01J20/28B01J20/30B01D71/78B01D69/10B01D67/00C07D491/044
Inventor 龚国利赵婷峰李慧
Owner SHAANXI UNIV OF SCI & TECH
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