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Recycling method of cortisone acetate biological catalysis cyclodextrin medium

A technology of cortisone acetate and biocatalysis, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of reducing application costs, simple process, and cost saving

Active Publication Date: 2014-04-02
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the recycling technology of cyclodextrin in the biocatalytic reaction of hydrophobic compounds has not been reported at home and abroad.

Method used

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  • Recycling method of cortisone acetate biological catalysis cyclodextrin medium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Compound C of cortisone acetate 1,2 dehydrogenation reaction

[0020] The microbial strain adopts Arthrobacter simplex TCCC11037, which can realize the C 1，2 The conversion of cortisone acetate to prednisone acetate by dehydrogenation.

[0021] Incline medium (g / L): glucose 10, yeast extract 10, agar 20, pH7.0

[0022] Seed medium (g / L): glucose 10, corn steep liquor 10, peptone 5, dipotassium hydrogen phosphate 2.5, pH 7.0

[0023] Fermentation medium (g / L): glucose 10, corn steep liquor 15, peptone 5, dipotassium hydrogen phosphate 2.5, pH 7.0

[0024] Arthrobacter simplex resting cell preparation:

[0025] The microorganisms were activated and cultured at 32°C, 160r / min, and 30 / 250mL loading for 24 hours, and the activated seeds were fermented and cultured at 30 / 250mL loading for 24 hours with 5% inoculum. The cells obtained from the fermentation culture were centrifuged at 5000 r / min, washed three times with PBS of pH 7.2, and resuspended in PBS buffer of pH 7....

Embodiment 2

[0031] Except the following content, other is the same as embodiment 1.

[0032] The cyclodextrin used in the biotransformation was 20mmol / L hydroxyethyl-β-cyclodextrin.

[0033] Cyclodextrin recycling process:

[0034] Centrifuge 50 mL of the conversion solution at 5000 r / min for 10 min to obtain the filtrate and filter cake, add 7 times the amount of chloroform to the filtrate, extract twice, and vacuum rotary evaporate the raffinate at 35 ° C to remove residual chloroform. The raffinate phase of dextrin was reused for the bioconversion of cortisone acetate. The results showed that the final conversion rate of CA was still over 94% after cyclodextrin was recycled for 5 times.

Embodiment 3

[0036] Except the following content, other is the same as embodiment 1.

[0037] The cyclodextrin used in the biotransformation was 50mmol / LL hydroxypropyl-β-cyclodextrin, and the substrate was 10g / L cortisone acetate.

[0038] Cyclodextrin recycling process:

[0039] Centrifuge 50 mL of the conversion solution at 5000 r / min for 10 min to obtain the filtrate and filter cake, add 3 times the amount of dichloromethane of the filtrate, extract twice, and vacuum rotary evaporate the raffinate at 30 ° C to remove residual dichloromethane. The raffinate phase of dextrin was reused for the bioconversion of cortisone acetate. The results showed that the final conversion rate of CA was still above 96% after cyclodextrin was recycled for 5 times.

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Abstract

The invention relates to a recycling method of a cortisone acetate biological catalysis cyclodextrin medium, and belongs to the technical field of biotransformation. The recycling method comprises the following steps of: processing a cyclodextrin-containing transfer solution by filtering and extracting with an organic solvent; removing the residual organic solvent from a cyclodextrin-containing extraction phase; adding to perform biological catalysis of a water repellent compound again, thus realizing the recycle of high-price cyclodextrin as well as environmentally-friendly and clean production of biological catalysis. The recycling method saves the production cost, and has a wide application prospect.

Description

Technical field: [0001] The invention belongs to the technical field of biocatalysis. Specifically relates to a recycling method of cyclodextrin medium in cortisone acetate biocatalysis. Background technique: [0002] Cyclodextrin is a general term for a series of cyclic oligosaccharides produced by cyclodextrin glucose residue transferase on starch, glycogen, maltooligosaccharides and other glucose polymers. Among them, α-, β-, γ-cyclodextrins are more studied and have important practical significance. Cyclodextrin has the characteristics of being hydrophilic outside the ring and hydrophobic inside the ring, and can provide a hydrophobic binding site to include such as inorganic, organic molecules and gas molecules, etc., and change the chemical and physical properties of the inclusion compound. On the basis of natural cyclodextrin, modify it such as esterification, etherification, alkylation, hydroxylation, etc. to obtain cyclodextrin derivatives, which can improve some ...

Claims

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Application Information

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IPC IPC(8): C12P33/02C12R1/06
CPCY02P20/584
Inventor 王敏申雁冰梁静婷赵清骆健美郑宇
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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