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Product capable for reducing serum cholesterol level by using whey protein peptide

A whey protein peptide and cholesterol technology, which is applied in the preparation, separation and purification of whey protein peptide, can solve the problem that the active peptide cannot resist the digestion of the gastrointestinal tract, and achieve the avoidance of health risks, high safety and good application The effect of prospects and market prospects

Active Publication Date: 2014-04-02
NORTHEAST AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0005] However, many active peptides in the body cannot resist the digestion of the gastrointestinal tract, and cannot reach the target site to exert physiological effects

Method used

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  • Product capable for reducing serum cholesterol level by using whey protein peptide
  • Product capable for reducing serum cholesterol level by using whey protein peptide
  • Product capable for reducing serum cholesterol level by using whey protein peptide

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Embodiment Construction

[0031] Ingredients: Whey Protein Concentrate WPC80; Alkaline Protease: 2×10 5 U / g.

[0032] 1. Take 25 g of whey protein concentrate raw material, dissolve it in 500 mL of distilled water, and heat it to 90 °C in a water bath for 10 min. Cool down to 55 °C, adjust the pH to 9.0 with 1M NaOH, add 1 g of alkaline protease, and keep adding 1M NaOH during the hydrolysis process to maintain the pH at 9.0, and the hydrolysis time is 8 h. After hydrolysis, heat up to 90°C for 10 minutes to inactivate enzyme activity, centrifuge at 4000×g for 15 minutes, take supernatant, obtain crude polypeptide liquid and freeze-dry to obtain hydrolyzed peptide powder.

[0033]2. Make the hydrolyzed peptide powder obtained in step 1 into a solution of 5 mg / mL, perform ultrafiltration with a membrane with a molecular weight cut-off of 10 kDa at a temperature of 30 °C and a pressure of 0.15 MPa, and collect the ultrafiltration permeate and cut-off solution, and their inhibition rate of cholesterol ...

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Abstract

The invention aims to deeply and systematically research active peptide capable of reducing serum cholesterol level by taking whey protein concentrate as a raw material, research a biological enzyme technology and a membrane fractional separation concentration and multiple chromatographic separation and purification technology, prepare whey protein hydrolytic peptide with small molecular weight and a function of reducing cholesterol, and provide theoretical basis and technical support for industrialized production. The invention adopts multiple chromatography to perform separation and purification, so the purity and the cholesterol micelle solubility inhibition ratio of the obtained component are higher; deionized water serves as eluent during gel filtration, so the deionized water is milder compared with the past salt eluent, a separation effect can be achieved well, salt ions are avoided, and health hidden danger is avoided. The obtained product has extremely high safety and no toxic or side effect, has a function of reducing serum cholesterol level, can easily serve as a functional factor to be added into various healthcare foods, can be applied to food and medicine industries and has a good market prospect.

Description

technical field [0001] The invention relates to a product and a preparation method for lowering serum cholesterol levels by utilizing whey protein peptides, and belongs to the field of biotechnology, and relates to a preparation method and separation and purification technology of whey protein peptides with the function of lowering serum cholesterol. Background technique [0002] In recent years, with the progress of human society and the development of science and technology, people's living standards and dietary nutrition levels have been greatly improved, but the hidden dangers of people's health have also been invisible, including heart and brain. Vascular disease. Hypertension, coronary heart disease, and cerebral thrombosis are common symptoms of cardiovascular and cerebrovascular diseases, and they are mainly caused by arteriosclerosis caused by hyperlipidemia. Because high cholesterol is extremely harmful to human health, and the cholesterol-lowering drugs synthesiz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/20C07K1/16A61P3/06
Inventor 李晓东马丽媛庄建鹏葛伟冷友斌蒋琛冯丽荣郑冬梅
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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