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Method for preparing breast milk fat substitute through lipase-catalyzed acidolysis of algae oil

A technology of breast milk fat and lipase, which is applied in the direction of fermentation to achieve the effect of improving resource utilization and extending the industrial chain

Active Publication Date: 2014-03-26
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Technical problem to be solved: the present invention aims at the existing HMFS used in infant formula and related problems of its preparation method, and uses oil-rich microalgae as raw material to provide a lipase-catalyzed transesterification of algal oil-sourced triglycerides prepared into The method of DHA-type HMFS with a fat composition close to that of breast milk and high nutritional value selectively enriches the content of unsaturated fatty acids such as DHA at the Sn-1 and 3 positions in triglycerides, and the prepared HMFS does not need to add additional DHA

Method used

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  • Method for preparing breast milk fat substitute through lipase-catalyzed acidolysis of algae oil
  • Method for preparing breast milk fat substitute through lipase-catalyzed acidolysis of algae oil
  • Method for preparing breast milk fat substitute through lipase-catalyzed acidolysis of algae oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This example illustrates the extraction of triglycerides from microalgae.

[0025] Mix the dried algae powder with the solvent ether, heat and extract for 0.5h under the assisted condition of ultrasound (200W), remove the residue by suction filtration, distill under reduced pressure at 45°C to obtain crude fat, dehydrate after centrifugation, and finally refine to obtain algae Triglycerides of oil.

[0026] After methyl esterification, it was detected by gas chromatography. Algal oil contains lauric acid (Lauric acid, C 14:0 ) and palmitic acid (C 16:0) saturated fatty acid (Saturated fatty acid, SFA), respectively 5.5% and 19.1%; in addition, unsaturated fatty acid (Unsaturated fatty acids, USFA) oleic acid (Oleic acid, C 18:1 ), linoleic acid (Linoleic acid, C 18:2 ), DPA (Docosapentenoic acid, C 22:5 ) and DHA (Docosahexaenoic Acid, C 22:6 ) content were 19.9%, 1.6%, 16.1% and 36.4%.

[0027] The solvent can also be ethanol, n-butanol, acetone, n-hexane, cyclo...

Embodiment 2

[0030] This example illustrates the process of lipase-catalyzed transesterification of algal oil (rich in triglycerides) and oleic acid.

[0031] Refined algae oil (98% triglyceride content) 9.5g and 2.82g oleic acid (1:1 molar ratio), add 0.37g (3% substrate weight) Sn-1, 3-position specific lipase Lipozyme RM IM , the reaction temperature is 90°C, and the reaction time is 10h.

[0032] After the reaction, take out 300 μL, add 50 mg porcine pancreatic lipase to hydrolyze for 5 min, extract with ether, take the organic layer for thin-layer chromatography, and scrape the bands of Sn-2 monoglyceride and triglyceride respectively. Add 2mL of n-hexane and 2mL of 0.5M KOH-methanol solution and react in a water-bath shaker at 65°C for 30min to carry out methyl esterification, and then perform gas chromatography detection, Sn-1, 2, 3 fatty acid content (Sn-2 FA content The FA at the sn-2 position accounts for the total content of the FA in the triglyceride) see Table 1.

[0033] Ta...

Embodiment 3

[0036] This example illustrates the process of lipase-catalyzed transesterification of algal oil (rich in triglycerides) and oleic acid.

[0037] Refined algae oil (98% triglyceride content) 9.5g and 14.1g oleic acid (1:5 molar), added 2.1g (9% substrate weight) Sn-1,3 position specific lipase Lipozyme RM IM, the reaction temperature is 65°C, and the reaction time is 3h.

[0038] After the reaction, take out 300 μL, add 50 mg porcine pancreatic lipase to hydrolyze for 5 min, extract with ether, take the organic layer for thin-layer chromatography, and scrape the bands of Sn-2 monoglyceride and triglyceride respectively. Add 2mL of n-hexane and 2mL of 0.5M KOH-methanol solution and react in a water-bath shaker at 65°C for 30min to carry out methylation, and then detect by gas chromatography.

[0039] Table 2. Sn-1, 2, 3 fatty acid content

[0040]

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Abstract

The invention discloses a method for preparing a breast milk fat substitute through lipase-catalyzed acidolysis of algae oil. According to the method, the triglyceride-rich algae oil and oleic acid are mixed and catalyzed by lipase to be subjected to acidolysis so as to synthesize the breast milk fat substitute suitable for direct usage by infants; the mixing mass ratio of the algae oil to the oleic acid is in a range of 1:1-1:9; the mass ratio of the lipase in a reaction system is in a range of 3%-12%; the reaction temperature is in a range of 25-90 DEG C; and the reaction time is in a range of 0.5 h-10 h. Most breast milk fat substitutes used in China at the present stage depend on imports, and the method is beneficial to domestic large-scale production and development, so that the import pressure is reduced. Compared with breast milk fat substitutes prepared from traditional raw materials, the method has the advantages that the algae oil which is safe in source and easy to obtain is used for preparing the breast milk fat substitute, the substitute is rich in polyunsaturated fatty acid such as DPA (docosapentaenoic acid) and DHA (docosahexaenoic acid), and the substitute is more beneficial to growth and development of the infants.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for preparing a breast milk fat substitute with high nutritional value by catalyzing the transesterification of triglyceride derived from algae oil by lipase. Background technique [0002] A good human milk fat substitute (HMFS) should function very similarly to natural human milk fat. As we all know, the intestinal tract of infants and young children is a nutritional transfer station for growth and development, and the digestion and absorption of nutrients required for production and development depend on healthy intestinal function. Adding HMFS to formula milk powder will change the molecular structure of fat, which can effectively reduce the possibility of calcium soap, thereby softening the stool, reducing the chance of intestinal obstruction or even constipation in infants; reducing the loss of nutrients, effectively improving infants' energ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
Inventor 王俊王旭东刘曦赵星宇吴福安
Owner JIANGSU UNIV OF SCI & TECH
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