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A method for compounding and synergizing Enterococcus faecalis and its application

A technology of Enterococcus faecalis and Enterococcus faecium, applied in the application field of microbial technology, can solve the problems of unstable product properties, low survival rate of live bacteria, unsatisfactory use effect, etc., and achieve obvious water quality stabilization effect and high bacterial survival rate , the effect of improving the water quality environment

Active Publication Date: 2015-12-30
湖北至正天辰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It solves the problems of low survival rate of live bacteria, unstable product properties and unsatisfactory use effect in the current Enterococcus faecalis preparations on the market

Method used

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  • A method for compounding and synergizing Enterococcus faecalis and its application
  • A method for compounding and synergizing Enterococcus faecalis and its application
  • A method for compounding and synergizing Enterococcus faecalis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A compounding and synergizing method of Enterococcus faecalis and its application, wherein the microbial compounding is prepared by the following raw materials in parts by weight:

[0032]

[0033] A method for compounding and synergizing Enterococcus faecalis and its application, the product preparation steps are:

[0034] a. Strains: Enterococcus faecalis, Enterococcus faecium, Streptococcus acidophilus, Saccharomyces cerevisiae.

[0035] b. Production of powdery composite microbial agent: mix Enterococcus faecalis, Enterococcus faecium, Streptococcus acidophilus, and Saccharomyces cerevisiae (the fineness of the bacterial powder is greater than 100 mesh) according to the above ratio to obtain a powdery composite microbial agent .

[0036] The yeast powder is purchased from Angel Yeast Co., Ltd., with a bacterial content of 20 billion / g;

[0037] The bacteria powders of Enterococcus faecalis, Enterococcus faecium and Streptococcus acidophilus were purchased from ...

Embodiment 6

[0038] Embodiment 6: a method for compounding and synergizing Enterococcus faecalis and its application, its product preparation steps are:

[0039] Weigh 4 to 5 parts by weight of powdery composite microbial agent, 5 to 6 parts by weight of live bacteria growth accelerator, the above materials are thoroughly mixed, dried at a low temperature of 40°C, cooled, and passed through a 40-mesh sieve. Bacteria packaging is made into a composite microbial agent, and the effective number of viable bacteria in the agent is greater than 10 billion / g.

[0040] The powdery composite microbial agent is Enterococcus faecalis, Enterococcus faecium, Streptococcus acidophilus, and Saccharomyces cerevisiae, according to 1-10 parts: 1-8 parts: 1-8 parts: 1-6 parts It is made by mixing the parts by weight ratio. The live bacteria reproduction accelerator includes lactose, fructooligosaccharide, chitosan, yeast powder, folic acid, citric acid, etc., according to 0.1-2.5 parts: 0.1-2.5 parts: 0.1-2.5...

Embodiment 7

[0041] Embodiment 7: A method for compounding and synergizing Enterococcus faecalis and its application, compared with other brands of Enterococcus faecalis preparations in the market, the steps are:

[0042] The organic matter decomposition ability, protease, amylase hydrolysis ability, growth rate and other performances of this Enterococcus faecalis preparation product and other enterococcus faecalis preparation products on the market were compared. The main results are as follows:

[0043] Table 1: Performance comparison table between the Enterococcus faecalis preparation of the present invention and the Enterococcus faecalis products on the market

[0044]

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Abstract

The invention relates to a method for compounding and synergizing Enterococcus faecalis and its application. The method for compounding and synergizing Enterococcus faecalis mainly includes compounding of microbial strains, compounding of living bacteria growth promoters, and the like. The microbial strains described in the invention are mainly composed of one or more mixtures of Enterococcus faecalis, Enterococcus faecium, Streptococcus acidophilus, and yeast; the living bacteria reproduction accelerator is mainly composed of xylitol, lactose, One or more of fructooligosaccharides, xylooligosaccharides, chitosan, yeast powder, folic acid and citric acid are mixed. The strain compounding synergistic method of the present invention realizes the synergistic effect among different microbial strains, accelerates the purification process of water body pollutants, and promotes the balance of logistics and energy flow in the breeding ecosystem. Using this product in the process of aquaculture can effectively balance the bacterial phase and algae phase of the water body, quickly decompose residual bait, feces, animal carcasses and other pollutants, improve the water quality of the aquaculture water body, increase the stability of the aquaculture water body, and prevent the aging of the aquaculture water body.

Description

technical field [0001] The invention belongs to the field of microbial technology application, and in particular relates to a compounding and synergizing method of Enterococcus faecalis, and also relates to the application of a compound microbial bacterial agent of Enterococcus faecalis in aquaculture. Background technique [0002] Enterococcus faecalis is also called streptococcus faecalis, its shape is streptococcus or spherical, the diameter of the bacteria is between 0.3 μm and 0.7 μm, without spores, the colony formed on the broth agar plate after dilution is round, milky white, surface The bumps are moist, shiny, with neat edges, most of them are arranged in pairs or short chains, and usually do not move. Enterococcus faecalis is a representative species of enterococci in group D streptococci. Usually, the so-called Enterococcus faecalis mainly includes Streptococcus faecalis, Streptococcus faecalis liquefaction subspecies, Streptococcus faecalis enzyme-producing subsp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/18C02F3/34C12R1/865C12R1/46C12R1/01
Inventor 荣克明张学振冯宪斌
Owner 湖北至正天辰生物科技有限公司
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