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Separation method for macadimia nut fine protein

A separation method and high-quality protein technology, which is applied in the field of separation of high-quality protein from macadamia nuts, can solve the problems of low extraction rate of macadamia nut protein, low protein purity, etc., achieve good dissolution effect, improve extraction efficiency, and improve the effect of purity

Inactive Publication Date: 2014-03-26
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problems of low extraction rate of macadamia nut protein and low protein purity, the present invention provides a method for separating high-quality protein from macadamia nuts, comprising the following steps:

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The macadamia nut kernels are crushed in a 2000r / min high-speed grinder for 20 seconds, and the crushed macadamia nuts are dried with hot air to a water content of 4%, then wrapped with filter cloth and put into the barrel of a hydraulic oil press, and the pressure is adjusted to 50MPa , pressed 3 times at 45-50°C to collect macadamia nut meal. Weigh 1 kg of macadamia nut meal, add 8 kg of pure water, stir evenly, adjust the pH value to 9.2 with 1mol / L NaOH solution, stir and extract in a water bath environment of 55°C for 60 minutes, and the stirring speed is 400r / min; Centrifuge at 6000r / min for 10min, add 1 mol / L hydrochloric acid evenly to the separated supernatant to pH 4.6, centrifuge at 7000r / min for 20min; add purified water to the precipitate until the protein concentration is 20%, use a 35MPa homogenizer After the homogenization treatment, spray drying is carried out to obtain the macadamia nut fruit protein, the spray drying inlet air temperature is 150°C, th...

Embodiment 2

[0024] The macadamia nut kernels are crushed in a 2000r / min high-speed grinder for 20 seconds, and the crushed macadamia nuts are dried with hot air to a water content of 4%, then wrapped with filter cloth and put into the barrel of a hydraulic oil press, and the pressure is adjusted to 50MPa , pressed 3 times at 45-50°C to collect macadamia nut meal. Weigh 1 kg of macadamia nut meal, add 8 kg of pure water, stir evenly, adjust the pH value to 9.2 with 1mol / L NaOH solution, stir and extract in a water bath environment of 55°C for 60 minutes, and the stirring speed is 400r / min; Centrifuge at 6000r / min for 10min, add 1 mol / L hydrochloric acid evenly to the separated supernatant until the pH value is 4.6, centrifuge at 7000r / min for 20min, add 6 times the mass of pure water at 45°C to the precipitate, and wash the mixture with 2500r / L After dispersing with a disperser for 10 minutes, centrifuge at 4000r / min for 10min to collect the precipitate, add 6 times the mass of pure water ...

Embodiment 3

[0026] Macadamia nut kernels are crushed in a 2000r / min high-speed grinder for 20 seconds, and the crushed macadamia nuts are dried by infrared rays to a water content of 4%, the infrared wavelength is 5 μm, the heat source temperature is 500 ° C, and the distance between the heat source and the nuts is 10 cm; Wrap the macadamia nuts with a filter cloth and put them into the barrel of a hydraulic oil press, adjust the pressure to 50MPa, press at 45-50°C for 3 times, and collect the macadamia nut meal. Weigh 1 kg of macadamia nut meal, add 8 kg of pure water, stir evenly, adjust the pH value to 9.2 with 1mol / L NaOH solution, stir and extract in a water bath environment of 55°C for 60 minutes, and the stirring speed is 400r / min; Centrifuge at 6000r / min for 10min, add 1 mol / L hydrochloric acid evenly to the separated supernatant until the pH value is 4.6, centrifuge at 7000r / min for 20min, add 6 times the mass of pure water at 45°C to the precipitate, and wash the mixture with 250...

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Abstract

The invention relates to a separation method for macadimia nut fine protein, which mainly comprises the following steps: (1) smashing macadimia nuts, and separating macadimia nut fruit oil via infrared treatment and the hydraulic cold pressing method; (2) mixing the obtained macadimia nut dreg and purified water, and processing the mixture through a colloid mill and a homogenizer, performing hardening and tempering, and then performing dynamic ultrahigh pressure processing; (3) adjusting the pH of fruit pulp to the isoelectric point, separating, repetitively washing precipitate with warm water, and then performing low-temperature spray drying to obtain the macadimia nut fine protein. The technology is continuous and compact, the production yield is high and the quality of the macadimia nut fine protein is good.

Description

technical field [0001] The invention relates to a method for separating fruit protein, in particular to a method for separating high-quality protein from macadamia nuts. Background technique [0002] Macadamia nuts belong to the genus Macadamia of the family Longanaceae, also known as macadamia nuts, macadamia nuts, and Queensland chestnuts. The edible part of the macadamia nut is the kernel, which is crispy, smooth, tender and delicious, with a unique buttery aroma. It is the best quality edible fruit in the world, and has the reputation of "Queen of Dried Fruits" and "King of Nuts in the World". Macadamia nuts are rich in nutrients, with an oil content of 78%, protein 9%, and 8 essential amino acids, as well as minerals and vitamins. Macadamia nut oil contains more than 80% monounsaturated fatty acids, mainly oleic acid and palmitoleic acid, so macadamia nuts are mainly used for frying oil in addition to being eaten as dried fruit. The fried macadamia nut meal protein is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/14
Inventor 钟业俊刘成梅刘伟徐梦涵杨晓会
Owner NANCHANG UNIV
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