Heat shock transcription factor gene AtHSFA6a, coding protein, and applications thereof

Abstract

The invention belongs to the plant molecular biology and transgenosis related technical fields, concretely relates to verification of high temperature resistant function of arabidopsis thaliana heat shock transcription factor gene AtHSFA6a, and provides applications of the gene in cultivation of high temperature resistant new varieties of plants. The model plant arabidopsis thaliana is employed as a material, the AtHSFA6a gene T-DNA is inserted into a homozygous mutant strain salk-045608, and the functions of the AtHSFA6a gene in the aspect of plant high temperature resistance are studied. Experiment results show that, the AtHSFA6a gene knockout mutant shows a sensitive phenotype under heat shock stress, and is significantly or highly significantly different from the wild type at aspects of physiological indices of root length, fresh weight of an overground part, conductivity, osmotic pressure, malondialdehyde content, SOD enzymatic activity and the like, which shows that the AtHSFA6a gene plays an important role in the signal transduction pathway of response to heat shock stress of plants, and predicts that overexpression of the gene can raise heat resistance of plants, which has large economic values in plant stress resistance molecular breeding.

Description

Technical field: [0001] The invention relates to the field of plant molecular biology, in particular to the verification of the new high temperature resistance function of Arabidopsis heat shock transcription factors (Heat Shock Transcription Factors, HSFs) family member AtHSFA6a, and the application of the gene in the cultivation of new high temperature resistant plant varieties. Background technique: [0002] The change of temperature has an important impact on the growth and development of plants. Appropriate temperature can ensure or promote the normal growth and development of plants, while unsuitable temperature conditions will inhibit the growth of plants and even cause plant death. Since the 20th century, with the rapid growth of world population and the continuous deepening of industrialization, the greenhouse effect has become more and more serious, and the gradual increase of global temperature has become an important limiting factor for the normal growth, developm...

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Problems solved by technology

However, there has never been any report on the effect of this gene on the growth and development of roots and shoots under heat stress conditions, as well as on the physiological and biochemical effects of plants. An in-depth analysis of the function of this gene from the perspective of physiological, biochemical and molecular genetics may provide people with a way to use molecular breeding. It is an effective way to cultivate new varieties of crops with enhanced high temperature resistance or other adversity capabilities

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