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Method for producing vancomycin hydrochloride by utilizing vancomycin fermentation broth

A technology of vancomycin hydrochloride and vancomycin, which is applied in the field of synthesis and antibiotic extraction, can solve the problems of prolonging the extraction time, low yield of finished products, and high production cost, and achieve the effects of reducing costs, simplifying the extraction process, and shortening the production cycle

Active Publication Date: 2014-03-19
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are invention patents and literature reports on the extraction and purification of vancomycin by macroporous adsorption resin adsorption and elution technology at home and abroad, but the main problem is that the fermentation broth needs to be filtered after pretreatment, and the purification and extraction The production process is complicated, which prolongs the extraction time; the yield of finished products is low, generally around 60%, resulting in high production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Vancomycin fermentation broth 10m 3 , the titer is 8.4g / L, the pretreatment tank is turned on to stir, the speed is controlled at 80r / min, and the temperature is controlled at 20-21°C. Add 1 OL of chemical penetrant. After stirring for 20 minutes, let stand for 100 minutes. Use 20% hydrochloric acid to first lower the pH of the fermentation broth to 5.5 and let it stand for 30 minutes, then lower the pH to 5 and let it stand for 30 minutes. Finally the pH dropped to 4.5. To control the pH adjustment speed, it is required that the pH drop does not exceed 0.3 every 10 minutes. After the pH adjustment was completed, 20 kg of adsorbent was added. The stirring speed was controlled at 50r / min. The adsorption time was controlled at 90min. 1 kg of sodium sulfide was added to the fermentation broth, the stirring speed was controlled at 50 r / min, and the stirring time was controlled at 60 min. After the pretreatment of the fermentation broth is completed, add drinking wate...

Embodiment 2

[0037] Fermentation broth 10m 3, the potency is 8.1g / L, the pretreatment tank is turned on to stir, the speed is controlled at 83r / min, and the temperature is controlled at 21-22°C. Add chemical penetrant 15L. After stirring for 20 minutes, let stand for 105 minutes. Use 20% hydrochloric acid to first lower the pH of the fermentation broth to 5.5 and let it stand for 30 minutes, then lower the pH to 5 and let it stand for 30 minutes. Finally the pH dropped to 4.5. To control the pH adjustment speed, it is required that the pH drop does not exceed 0.3 every 10 minutes. After the pH adjustment was completed, 25 kg of adsorbent was added. The stirring speed was controlled at 53r / min. The adsorption time was controlled at 92min. 1.2 kg of sodium sulfide was added to the fermentation broth, the stirring speed was controlled at 52 r / min, and the stirring time was controlled at 62 min. After the pretreatment of the fermented liquid is completed, add 1.2m of drinking water 3 ....

Embodiment 3

[0045] Fermentation broth 10m 3 , the titer is 8.3g / L, the pretreatment tank is turned on to stir, the speed is controlled at 85r / min, and the temperature is controlled at 22-23°C. Add chemical penetrant 20L. After stirring for 20 minutes, let stand for 110 minutes. Use 20% hydrochloric acid to first lower the pH of the fermentation broth to 5.5 and let it stand for 30 minutes, then lower the pH to 5 and let it stand for 30 minutes. Finally the pH dropped to 4.5. To control the pH adjustment speed, it is required that the pH drop does not exceed 0.3 every 10 minutes. After the pH adjustment was completed, 35 kg of adsorbent was added. The stirring speed was controlled at 55r / min. The adsorption time was controlled at 95min. 1.5 kg of sodium sulfide was added to the fermentation broth, the stirring speed was controlled at 55 r / min, and the stirring time was controlled at 65 min. After the pretreatment of the fermentation broth is completed, add drinking water 1.5m 3 . ...

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Abstract

A method for producing vancomycin hydrochloride by utilizing a vancomycin fermentation broth comprises pretreatment of the vancomycin fermentation broth, inorganic ceramic membrane filtration, ultrafiltration, nanofiltration, crystal water-washing, acid-base neutralization and vacuum freeze drying. The method helps to realize the stable efficient production of vancomycin hydrochloride. Also the method helps to improve the extraction yield, shorten the production period, reduce the cost and improve the yield of vancomycin hydrochloride.

Description

technical field [0001] The invention belongs to the technical field of antibiotic extraction and synthesis, and in particular relates to a method for producing vancomycin hydrochloride by using vancomycin fermentation liquid. Background technique [0002] Vancomycin is a glycopeptide antibiotic isolated from the fermentation broth of a strain called Amycolatopsis orientalis screened from Indonesian soil and has anti-Gram-positive bacteria. It is currently the drug of choice for the treatment of severe infectious diseases caused by MRSA clinically, and is therefore praised by international antibiotic experts as "the last line of defense for humans against stubborn drug-resistant strains" and "the ace antibiotic". [0003] At present, there are invention patents and literature reports on the extraction and purification of vancomycin by macroporous adsorption resin adsorption and elution technology at home and abroad, but the main problem is that the fermentation broth needs t...

Claims

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Application Information

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IPC IPC(8): C07K9/00C07K1/30
Inventor 任勇
Owner 宁夏泰瑞制药股份有限公司
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