Factory-like production method of silkworm cordyceps

Abstract

The invention provides a factory-like production method of silkworm cordyceps. The method is characterized by comprising the steps as follows: separating fruiting bodies of cordyceps militaris, sterilizing and washing the fruiting bodies, putting the fruiting bodies on a slant culture medium, and placing the slant culture medium in an incubator for culture; placing a PDA (potato dextrose agar) culture medium in a shake flask, placing white mycelia in the shake flask for shake culture, inoculating the white mycelia on a rice culture medium, after the white mycelia grow over the rice culture medium, taking out the mycelia, adding a glucose liquid, mashing the mycelia, and obtaining a nutrition fungus suspension as an inoculation fluid; stabbing and injecting pleopod parts of silkworm larvae with a medical injector sucked with the inoculation liquid to obtain the inoculated silkworm larvae, obliquely placing the silkworm larvae on the sterilized rice culture medium, performing light-shielding variable-temperature culture on the silkworm larvae firstly, and performing light culture after white hyphae grow over the larvae; and collecting the silkworm cordyceps. The factory-like production method is simple in technology, the mass production can be realized, and the silkworm cordyceps with shapes similar to natural cordyceps sinensis and medium values basically identical with the cordyceps sinensis can be produced.

Description

technical field [0001] The invention is related to indoor artificial cultivation of Cordyceps sinensis, and is an industrialized production process of Cordyceps sinensis by using silkworm larvae to produce Cordyceps fungus indoors. The product produced by this process is the conjugation of insect body (silkworm larvae) and grass body (fruiting body), which is similar in shape to naturally growing Cordyceps sinensis, and its main components and medicinal value are basically the same. Background technique [0002] The Chinese patent with the publication number CN103262751A discloses a method for cultivating Cordyceps silkworms by infecting silkworm larvae with the Cordyceps militaris strain, which includes the following steps: (1) selection of silkworms: selecting silkworms of the fifth instar 3rd to 5th days as hosts; 2) Strain preparation: inoculate the strain of Cordyceps militaris in the liquid culture medium, and after shaking culture, filter the sterile gauze to obtain t...

AI Technical Summary

Problems solved by technology

The weak point of this method is, 1) put silkworm into the silkworm pretreatment time of 20~50min under ultraviolet sterilization too long; Bombyx mori outflow body fluid; 3) Put the inoculated silkworm into the nutrient soil and place it in the dark at 5°C-12°C for cultivation, which will make the silkworm body flow out more body fluid and only the skin of the silkworm body will remain

The disadvantage of this patent is that after the physical and chemical biological treatment of Cordyceps sinensis, the strain inoculated into the silkworm body is difficult to grow in the silkworm body, the success rate is extremely low, and mass production cannot be formed.

[0004] The disadvantage of the above-mentioned patents is that the key points of the growth of the Cordyceps fungus have not been really investigated and corresponding measures have not been taken, so the Cordyceps fungus cannot be produced in batches